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1

Genotyping of bovine beta-lactoglobulin (LGB) by PCR-SSCP technique

100%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
1997
|
vol. 38
|
issue 4
471-476
EN
A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.
2

Bovine kappa-casein (CASK) gene - molecular nature and application in dairy cattle breeding

88%
Kaminski S.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 2
176-196
EN
The bovine kappa-casein (CASK) gene is considered a potential marker for quantitative trait loci (QTL) in dairy cattle.A large amount of research has been performed to explore the nature and variation of the CASK gene and its possible applications in cattle breeding.The purpose of this review is to sum up the knowledge of all known aspects of the CASK gene; molecular structure and function, poltmorphism and allele frequency, methods of genotyping and possibilities of the use of CASK polymorphism in dairy cattle breeding.
3

Simultaneous identification of ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) genotypes with the multiplex PCR-RFLP method in Polish Large White and Polish Landrace pigs

75%
Kaminska S., Rusc A., Wojtasik K.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
331-335
EN
A method allowing simultaneous genotyping of two loci: ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) is presented. In multiplex PCR amplification, two amplicons were simultaneously produced: a 272 bp fragment of RYR1 gene and a 185 bp fragment of ESR gene and were then subjected to ?one-tube? restriction enzyme digestion with Hin6 I and Ava I, respectively. A total of 122 Polish Large White and Polish Landrace pigs were genotyped by this method, demonstrating its reliability, convenience and lower costs. This method may be useful in the wide-scale genotyping of both loci in pig breeding programmes.
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