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1

Molecular identification of new varieties of Nierembergia linariaefolia (Graham), a native Argentinean ornamental plant

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Escandon A. S., Zelener N., Perez_ d. l. T. M., Soto S.
Journal of Applied Genetics
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2007
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vol. 48
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issue 2
115-123
EN
Six Nierembergia linariaefolia clones were selected for ornamental traits during a native germplasm development program. For fingerprinting diagnosis, 13 anchored inter-simple sequence repeat (ISSR) primers and 6 amplified fragment length polymorphism (AFLP) primer-enzyme combinations were used. Both markers revealed high levels of polymorphism, enabling genetic discrimination of the accessions analyzed by using 443 informative ISSRs and 541 AFLP markers. Both molecular techniques are suitable for monitoring genetic diversity in Nierembergia linariaefolia and, under our experimental conditions, they showed correlation coefficients of 0.629 for similarity matrices and of 0.649 in the cophenetic matrices. These results suggest that ISSRs are a good choice for DNA analysis in N. linariaefolia when simple manipulation and a low budget are required.
2

Electrophoretic seed albumin patterns in the Vicia sativa L. aggregate

100%
Przybylska J., Zimniak-Przybylska Z., Krajewski P.
Journal of Applied Genetics
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2000
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vol. 41
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issue 3
139-149
EN
Electrophoretic analysis of seed albumins covered 201 accessions representing six subspecies of Vicia sativa L.: nigra, amphicarpa, incisa, sativa, macrocarpa and cordata. At least five individuals of each accession were examined separately. All the taxa under study showed variation with respect to albumin banding patterns, which was due to differences between accessions and individual variation within accessions. The number of albumin bands distinguished in particular taxa varied from 13 to 19. The statistical analysis of the electrophoretic data consisted in hierarchical grouping by the UPGMA method based on EUCLIDEAN distances. In the case of subsp. nigra and subsp. sativa, accessions originating from North Africa tended to form a group showing some distinctness from the remaining accessions. As to relationships among the studied taxa, subsp. nigra and subsp. cordata were shown to be the most closely related and their affinities to other members of the V. sativa aggregate were decreasing in the following order: subsp. macrocarpa, subsp. sativa, subsp. amphicarpa, subsp. incisa. The obtained results are discussed with reference to taxonomic relationships among the members of the V. sativa aggregate.
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