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1

Transfer of genes from Dasypyrum villosum (Haynaldia villosa L.) to Secale cereale L.

100%
Apolinarska B., Gruszecka D.
Biotechnologia
|
2001
|
issue 2
63-65
EN
The subject of the study was fertile diploid hybrids of rye, cv. Amilo (2n = RR = 14) with Dasypyrum villosum (Crimea, Ukraine) (2n = VV = 14) ? wild species belonging to Triticeae. translocation. Plants which morphologicaly resembled rye and which showed the highest The analysed strains were obtained using in vitro cultures with subsequent backcrossing of hybrid plants with pollen of ?Amilo? or D. villosum in the case of heterozygotic fertility and total protein content in kernels were selected during several generations. The small differences in the occurrence of heterochromatine bands in rye chromosomes were revealed by the differential staining technique at the stage of mitosis, between hybrid plants and ?Amilo? cv. Cells with mixoploid chromosome number 2n = 13, 2n = 14 and 2n = 15 were present in some plants. Neither substitution nor evident chromosome translocation were found in generations B3/F3 and B4/F2 under investigation. Therefore, the technique of nucleic acids hybridization in situ will be applied to detect fragments of D. villosum genotype introgressed into rye.
2

CRISPR elements in the Thermococcales: evidence for associated horizontal gene transfer in Pyrococcus furiosus

100%
Portillo M. C., Gonzalez J. M.
Journal of Applied Genetics
|
2009
|
vol. 50
|
issue 4
421-430
EN
The presence and distribution of CRISPR (clustered regularly interspaced short palindrome repeat) elements in the archaeal order Thermococcales were analyzed. Four complete genome sequences from the species Pyrococcus abyssi, P. furiosus, P. horikoshii, and Thermococcus kodakaraensis were studied. A fragment of the genome of P. furiosus was flanked by CRISPR elements upstream and by a single element downstream. The composition of the gene sequences contained in this genome fragment (positions 699013 to 855319) showed significant differences from the other genes in the P. furiosus genome. Differences were observed in the GC content at the third codon positions and the frequency of codon usage between the genes located in the analyzed fragment and the other genes in the P. furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea.
3

Gene therapy of cancer

88%
Gorny A., Wiznerowicz M., Mackiewicz A.
|
EN
Progress in genetic engineering has to lead to the development of efficient methods of transfer and expression of genes in eucariotic cells.This technology has been employed to kill cancer cells.Five major strategies of cancer gene therapy have been proposed and are currently verified in clinical trials.They include: (1) genetic cellular vaccines, (2) siuicide genes, (3) multidrug resistance genes, (4) HLA genes tansfer into cancer cells, and (5) repair of defective suppresor genes and/or removal of activated oncogene.
4

Gene transfer from Aegilops ventricosa to Triticum aestivum

75%
Tyrka M., Stefanowska G., Brzezinski W.
Biotechnologia
|
2001
|
issue 2
57-62
EN
RAPD (Randomly Amplified Polymorphic DNA) and glutenin SDS-PAGE analyses were performed on wheat hybrid strains derived from multiple crosses of hybrids between Ae. ventricosa and T. durum with hexaploid wheat. We found Aegilops specific bands (G03580 and T02990) in two hybrid strains (VGPB and VGPAA) using RAPD method. The presence of Ae. ventricosa specific fraction of glutenin was shown in one wheat hybrid strain (VGPP).
5

Microspore culture of winter oilseed rape (Brassica napus L.) in conjunction with other in vitro technologies

75%
Cegielska-Taras T., Szala L., Bartkowiak-Broda I.
|
EN
Microspore culture in conjuction with other technologies such as selection, mutagenesis and transformation has been used for the production of novel genotypes of Brassica napus L. for crop improvement. The example of in vitro selection of microspore - derived embryos includes: a) ploidy level, b) seed oil composition (for example: high level of erucic acid), c) genotypes with restorer gene for CMS-ogura system (by means of isozyme marker PGI-2 ), d) herbicide resistant forms. Efficiency of microspore mutagnesis has been tested by the treatment of freshly isolated microspores with UV and MNU. Direct delivery of foreign gene to the microspores (microprojectile bombardment) combined with the use of Agrobacterium tumefaciens to microspore derived embryos seems to be a promising way of oilseed rape transformation.
6

The kidney and the bladder of transgenic animals as bioreactors?

63%
Zbikowska H. M.
Biotechnologia
|
2004
|
issue 1
20-31
EN
Mammary gland-specific expression of human genes and secretion of human proteins into the milk of transgenic farm animals provides an important tool for manufacturing of many valuable pharmaceuticals. More recently, attention has focused on urine-based expression systems as a much more cost-effective technology. Successful application of this technology, however, requires the definition of several crucial regulatory elements that direct production of the protein into the urinary tract. To date, the 5? flanking region of either the uroplakin II (UPII) or uromodulin (THP) genes were used to drive the expression of heterologous proteins in the bladder or kidney epithelium of the transgenic mice, respectively. Herein, the progress and current limitations in this field are presented. Other currently known urine-specific protein genes are also described.
7

Flow of genetic material

44%
Dixon B.
Biotechnologia
|
1993
|
issue 4
116-
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