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EN
Alloantibodies detecting antigenic determinats called BgC1 and AmiF1 were obtained in cattle.The analysis of determinants' inheritance was done on 1754 offsprings using 72 sires and 1740 dams.The molecule-carriers of both traits were characterized using serological and physico-chemical methods.It was found that identified determinants are markers of beta-globulin and alpha-microglobulin proteins, and of autosomal, dominant genes BgC1 and AmiF1 from independent loci which control them.
EN
Since the first reports of successful plant transformation appeared, there have been steady improvements of the transformation methods. Nowadays, transgenic plants without the incorporation of selection genes for antibiotic or herbicide resistance would be the only ones acceptable to the public, so elimination of these genes from transgenic crops prior to their field release and commercialization seems inevitable. Several strategies have been developed to generate marker-free transgenic plants, including: positive selection, alternative marker genes, co-transformation, site-specific recombination, transpozon-mediated approaches and intrachromosomal homologous recombination. The efficiency of these methods is various as comparing to the traditional marker assisted selection - higher in case of alternative procedures and lower in others.
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