Search results

1

Homocysteine level in urine of autistic and healthy children

100%

Kałużna-Czaplińska J., Michalska M., Rynkowski J.

Acta Biochimica Polonica

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2011

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vol. 58

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issue 1

31-34

EN

Homocysteine is an amino acid which plays several important roles in human physiology and is an important biomarker for possible deficiencies of various vitamins (vitamin B6 and B12, folic acid). In this work GC-MS method was used to determine the levels of homocysteine in the urine of autistic and healthy children. The levels of homocysteine in urine samples from 34 autistic and 21 healthy children were 2.36 ± 1.24 and 0.76 ± 0.31 (mmol∙mol-1 creatinine), respectively. The higher level of homocysteine in autistic children may indicate deficiencies of folic acid and vitamins B6 and B12 in nutrition of these children. The results of this work were taken into consideration in the nutrition of autistic children treated in the Navicula Centre of Diagnosis and Therapy of Autism in Łódź (Poland).

2

Characterization of Catechins from Smilax domingensis Willd. in Cuba

88%

Soledispa P. A., González J., Cuéllar A., Pérez J., Monan M.

World News of Natural Sciences

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2019

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vol. 23

297-305

EN

Gas Chromatography coupled with mass spectrometry was applied to study the fragmentation of selected flavonoids from the ethanolic extract of Smilax domingensis Willd., Smilacaceae, known as zarzaparrilla, after Soxhlet extraction during 20 hours. Compounds belonging to one of the major subgroups found in common plants, i.e. flavanols were studied. Following solvent extraction and derivatization using BSTFA, possibly two different metabolites from the same chemical group were characterized in one analytical run: (+)-catechin and (-)-epicatechin. For the very first time, qualitative data on these analytes in the drug were determined after detailed validation of a sensitive, cheap and reliable GC-MS method.

3

TG/DTG/DTA, FTIR and GC/MS Studies of Oil Sand for Artistic and Precision Foundry with the Emission of Gases Assessment

75%

Żymankowska-Kumon S., Grabowska B., Bobrowski A., Drożyński D., Kaczmarska K.

Archives of Foundry Engineering

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2017

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vol. 17

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issue 4

4

13C NMR and mass spectrometry of soil organic matter

75%

Ivanova G., Randall E.

Open Chemistry

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2003

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vol. 1

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issue 1

10-27

EN

Liquid state, high resolution 13C NMR spectroscopy and mass spectrometry were used to study the composition and structure of soil organic matter (SOM) using soil extracts from two long-term experiments at the Rothamsted Experimental Station. Both one- and two-dimensional NMR techniques were applied. 13C NMR sub-spectra of the CHn (n=0...3) groups, obtained by the Distortionless Enhancement by Polarisation Transfer (DEPT) technique, were used for the elucidation of the qualitative and quantitative composition of humic and fulvic acids in the soils. The chemical structure of SOM was further analysed at the molecular level through Fast Atom Bombardment Mass Spectrometry (FABMS) and Gas Chromatography-Mass Spectrometry (GC/MS). Humic and fulvic extract results were not only compared to each other, but also to the solid state 13C NMR results for the complete soil sample.

5

Analysis of the Compounds from the BTEX Group, Emitted During Thermal Decomposition of Alkyd Resin

75%

Kubecki M., Dańko R., Żymankowska-Kumon S., Grabowska B., Bobrowski A., Holtzer M.

Archives of Foundry Engineering

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2012

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issue 3

6

Oznaczanie niesteroidowych leków przeciwzapalnych w surowicy ludzkiej techniką GC/MS: optymalizacja procedury ekstrakcyjnej leków

63%

Kurkiewicz S., Dzierżęga-Lęcznar A., Stępień K.

Annales Academiae Medicae Silesiensis

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2011

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vol. 65

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issue 3

28-33

EN

Determination of non-steroidal anti-infl ammatory drugs (NSAIDs) in human and animal physiological fl uids requires the use of appropriate pro-cedures permitting rapid and effi cient recovery of the drugs from biological matrices. The aim of this study was to compare various methods of NSAIDs extraction from human serum that is essential for GC/MS analysis of the drugs. Commercially available biochemistry control human serum supplemented with ibuprofen, paracetamol, fl urbiprofen, nabumeton, naproxen, ketoprofen, mefenamic acid and diclofenac sodium salt was used. Solid phase extraction, HybridSPE- -PPT technology and liquid-liquid extraction were applied for NSAIDs isolation from the serum. GC/MS analysis of the drugs was preceded by their derivatization to trimethylsilyl esters with BSTFA. The highest extraction effi ciency and the best separation of the drugs from endogenous components of the serum were achieved using the solid phase extraction on octadecyl-bonded silica.

PL

Oznaczanie niesteroidowych leków przeciwzapalnych (NLPZ) w płynach fizjologicznych ludzi i zwierząt wymaga zastosowania szybkich i wydajnych procedur izolacji tych leków z biologicznej matrycy. Celem pracy było porównanie różnych metod ekstrakcji NLPZ z surowicy ludzkiej, jako etapu poprzedzającego analizę tych leków techniką GC/MS. W badaniach wykorzystano dostępną handlowo surowicę stosowaną jako materiał kontrolny w oznaczeniach biochemicznych, do której dodano ibuprofen, paracetamol, flurbiprofen, nabumeton, naproksen, ketoprofen, kwas mefenamowy oraz sól sodową diklofenaku. W celu wyizolowania NLPZ z surowicy zastosowano ekstrakcję do fazy stałej, technikę HybridSPE- -PPT oraz ekstrakcję ciecz-ciecz. Leki poddano derywatyzacji do pochodnych trimetylosililowych za pomocą BSTFA, a następnie analizowano techniką GC/MS. Najwyższą wydajność ekstrakcji oraz najlepszy stopień oddzielenia NLPZ od endogennych składników surowicy uzyskano metodą ekstrakcji do fazy stałej w postaci żelu krzemionkowego z aktywnymi grupami oktadecylowymi.

7

Influence of the Hardener on the Emission of Harmful Substances from Moulding Sands with Furan Resin in the Pyrolysis Process

63%

Holtzer M., Dańko R., Żymankowska-Kumon S., Bobrowski A., Kmita A.

Archives of Foundry Engineering

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2016

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issue 1

8

Application of the pyrolytic methylation to GC/MS profi ling of short-chain fatty acids in selected pharmaceutical preparations

63%

Chodurek E., Kurkiewicz S., Dzierżęga-Lęcznar A., Stępień K., Dzierżewicz Z.

Annales Academiae Medicae Silesiensis

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2011

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vol. 65

|

issue 3

16-20

EN

Lactic Acid Bacteria (LAB) belong to the normal fl ora of human alimentary tract and vaginal epithelium. The main products of LAB metabolism are short chain fatty acids (SCFA), which are known to have signifi cant infl uence on human health. The aim of this study was to examine the suitability of direct pyrolytic methylation in GC/MS profi ling of SCFA in the selected pharmaceutical preparations of LAB. For method optimization, standard SCFA samples consisting of acetic, propionic, butyric and lactic acids were pyrolyzed under various temperature conditions in the presence of methanolic solution of tetramethylammonium hydroxide (TMAH) as a derivatizing reagent. It was demonstrated that pyrolytic derivatization of standard SCFA to methyl esters was the most effi cient with the use 10% TMAH and the pyrolytic fi laments with Curie temperature of 480°C, when the pyrolysis cell was kept at 150°C. It was shown that the method is suitable for GC/MS qualitative analysis of SCFA profi le in the pharmaceutical preparations that contain LAB in lyophilized form.

PL

Bakterie kwasu mlekowego (Lactic Acid Bacteria – LAB) należą do naturalnej flory bakteryjnej zasiedlającej przewód pokarmowy oraz nabłonek pochwy człowieka. Głównym produktem metabolizmu LAB są krótkołańcuchowe kwasy tłuszczowe (short-chain fatty acids – SCFA) wywierające istotny wpływ na zdrowie człowieka. W pracy poddano ocenie przydatność techniki bezpośredniej metylacji pirolitycznej w oznaczaniu profi lu SCFA w wybranych preparatach farmaceutycznych LAB techniką GC/MS. W celu optymalizacji metody, wzorcowe próbki SCFA zawierające kwas octowy, propionowy, masłowy i mlekowy poddano pirolizie w obecności metanolowego roztworu wodorotlenku tetrametyloamoniowego (TMAH) w różnych warunkach temperaturowych. Stwierdzono, że derywatyzacja analizowanych kwasów tłuszczowych do estrów metylowych zachodziła z największą wydajnością pod wpływem 10% TMAH, gdy zastosowano druty pirolityczne o temperaturze Curie 480°C, a komorę pirolizera utrzymywano w temperaturze 150°C. Wykazano, że zoptymalizowana metoda jest przydatna w prowadzonej techniką GC/MS analizie jakościowej profi lu SCFA w wybranych preparatach farmaceutycznych zawierających LAB w formie liofi lizatu.

Refine search results

Homocysteine level in urine of autistic and healthy children

Characterization of Catechins from Smilax domingensis Willd. in Cuba

TG/DTG/DTA, FTIR and GC/MS Studies of Oil Sand for Artistic and Precision Foundry with the Emission of Gases Assessment

13C NMR and mass spectrometry of soil organic matter

Analysis of the Compounds from the BTEX Group, Emitted During Thermal Decomposition of Alkyd Resin

Oznaczanie niesteroidowych leków przeciwzapalnych w surowicy ludzkiej techniką GC/MS: optymalizacja procedury ekstrakcyjnej leków

Influence of the Hardener on the Emission of Harmful Substances from Moulding Sands with Furan Resin in the Pyrolysis Process

Application of the pyrolytic methylation to GC/MS profi ling of short-chain fatty acids in selected pharmaceutical preparations