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1

Application of UV detection in HPLC in the total antioxidant potential assay

100%

Wantusiak P., Głód B.

Open Chemistry

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2012

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vol. 10

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issue 6

1786-1790

EN

HPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products. [...]

2

Modification of the deoxyribose test to detect strong iron binding

72%

Sadowska-Bartosz I., Galiniak S., Bartosz G.

Acta Biochimica Polonica

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2017

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vol. 64

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issue 1

195-198

EN

Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe2+ complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe2+ in the Fenton reaction, on the basis of examination of concentration dependence of deoxyribose degradation on Fe2+ concentration, at a constant concentration of a chelating agent.

3

Generation of ·OH initiated by interaction of Fe2+ and Cu+ with dioxygen; comparison with the Fenton chemistry.

72%

Urbański N. K., Beręsewicz A.

Acta Biochimica Polonica

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2000

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vol. 47

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issue 4

951-962

EN

Iron and copper toxicity has been presumed to involve the formation of hydroxyl radical (·OH) from H2O2 in the Fenton reaction. The aim of this study was to verify that Fe2+-O2 and Cu+-O2 chemistry is capable of generating ·OH in the quasi physiological environment of Krebs-Henseleit buffer (KH), and to compare the ability of the Fe2+-O2 system and of the Fenton system (Fe2+ + H2O2) to produce ·OH. The addition of Fe2+ and Cu+ (0-20 μM) to KH resulted in a concentration-dependent increase in ·OH formation, as measured by the salicylate method. While Fe3+ and Cu2+ (0-20 μM) did not result in ·OH formation, these ions mediated significant ·OH production in the presence of a number of reducing agents. The ·OH yield from the reaction mediated by Fe2+ was increased by exogenous Fe3+ and Cu2+ and was prevented by the deoxygenation of the buffer and reduced by superoxide dismutase, catalase, and desferrioxamine. Addition of 1 μM, 5 μM or 10 μM Fe2+ to a range of H2O2 concentrations (the Fenton system) resulted in a H2O2-concentration-dependent rise in ·OH formation. For each Fe2+ concentration tested, the ·OH yield doubled when the ratio [H2O2]:[Fe2+] was raised from zero to one. In conclusion: (i) Fe2+-O2 and Cu+-O2 chemistry is capable of promoting ·OH generation in the environment of oxygenated KH, in the absence of pre-existing superoxide and/or H2O2, and possibly through a mechanism initiated by the metal autoxidation; (ii) The process is enhanced by contaminating Fe3+ and Cu2+; (iii) In the presence of reducing agents also Fe3+ and Cu2+ promote the ·OH formation; (iv) Depending on the actual [H2O2]:[Fe2+] ratio, the efficiency of the Fe2+-O2 chemistry to generate ·OH is greater than or, at best, equal to that of the Fe2+-driven Fenton reaction.

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2 Acta Biochimica Polonica

1 Open Chemistry

1 2017

1 2012

1 2000

1 Bartosz G.

1 Beręsewicz A.

1 Galiniak S.

1 Głód B.

1 Sadowska-Bartosz I.

1 Urbański N. K.

1 Wantusiak P.

Application of UV detection in HPLC in the total antioxidant potential assay

Modification of the deoxyribose test to detect strong iron binding

Generation of ·OH initiated by interaction of Fe2+ and Cu+ with dioxygen; comparison with the Fenton chemistry.