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Antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Sea buckthorn (Hippophae rhamnoides L.)

100%
Kruczek M., Świderski A., Mech-Nowak A., Król K.
Acta Biochimica Polonica
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2012
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vol. 59
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issue 1
135-137
EN
Comparative analysis of antioxidant capacity was performed using FRAP and DPPH methods on extracts containing carotenoids acquired from fruits of Sea buckthorn. The examination included nine varieties of Sea buckthorn growing in the comparative cultivation. Conducted analysis allowed to compare the antioxidant capacity with carotenoids content measured with spectrophotometric and HPLC methods. Three of the examined cultivars indicating high antioxidant activity in both, FRAP and DPPH methods, also revealed highest ('Aromatnaya') and high ('Botanicheskaya', 'Arumnyj') total carotenoids content in HPLC analysis.
2
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THE CONTENT OF SELENIUM, POLYPHENOLS AND ANTIOXIDATIVE ACTIVITY IN SELECTED MEDICAL PLANTS FROM POLAND AND WESTERN UKRAINE

100%
Sotek Z., Białecka B., Pilarczyk B., Kruzhel B., Drozd R., Tomza-Marciniak A., Pilarczyk R., Lysak H., Bąkowska M., Vovk S.
Acta Poloniae Pharmaceutica - Drug Research
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2018
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vol. 75
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issue 5
1107-1116
EN
The three aims of the study were to: 1) evaluate the content of selenium and polyphenols, and to assess the reductive potential (FRAP test) and ability to deactivate ABTS•+ cation radicals in C. officinalis, H. perforatum, M. x piperita and S. marianum from Poland and Ukraine; 2) define the diversification in concentrations of selenium and polyphenols and in levels of ABTS activity and FRAP reductive potential within the examined species in relation to their location; and 3) evaluate the inter-dependencies between selenium, total polyphenol concentration, ABTS and FRAP activity, in the examined plants. We observed differences in the content of total polyphenols and antioxidative activity between the herb species and the location. Most selenium was found in M. x piperita and S. marianum from western Ukraine, with Polish H. perforatum from Pomerania and M. x piperita from Podlasie representing the highest TP content and antioxidative potential. A clear and significant relationship between the content of Se and antioxidative activity (ABTS and FRAP), and also between total polyphenols and antioxidative activity was noted only in H. perforatum. In C. officinalis a significant relationship was noted between Se and both total polyphenols and antioxidative potential. In M. x piperita a significant dependency was found between total polyphenols and antioxidative potential. The results of our research indicate a different role of selenium as an antioxidant in H. perforatum and C. officinalis, as well as polyphenols in H. perforatum and M. x piperita in dependence on the harvesting regions.
3
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Total Phenolic, Flavonoid Contents and Antioxidant Activities of Honey and Propolis Collected from the Region of Laghouat (South of Algeria)

86%
Bakchiche B., Habati M., Benmebarek A., Gherib A.
World News of Natural Sciences
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2017
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vol. 11
91-97
EN
The aim of this study was to determine total phenolic and flavonoid contents and to evaluate the antioxidant activities of two honeys and one propolis sample, collected from the region of Laghouat (South of Algeria). Total phenolic content were determined by using Folin-Ciocalteu reagent as gallic acid equivalent, while flavonoids content was assessed by means of the AlCl3 method as Rutin equivalent. Antioxidant activities of the honeys and propolis were examined by two different methods, namely, scavenging of free radical 2,2-diphenyl-1-picrylhydrazyl and reducing power. The antioxidant activities were compared with standard antioxidants such as Ascorbic acid, BHT and Trolox. The highest level of phenolic was 2385 mg Gallic acid per 100g sample, the highest level of flavonoid was 379 mg Rutin per 100g sample, while the highest protein content was 1177 mg per 100g sample, DPPH (0.026 mg/ml) and TEAC (0.0015) were detected especially in the propolis sample, indicating that it holds good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH and TEAC, indicating that in addition to total phenolic content, flavonoid and protein concentrations are good indicators of the antioxidant potential of propolis and honey.
4
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Approaches to imaging unfolded secretory protein stress in living cells

72%
Lajoie P., Fazio E. N., Snapp E. L.
Endoplasmic Reticulum Stress in Diseases
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2014
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vol. 1
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issue 1
EN
The endoplasmic reticulum (ER) is the point of entry of proteins into the secretory pathway. Nascent peptides interact with the ER quality control machinery that ensures correct folding of the nascent proteins. Failure to properly fold proteins can lead to loss of protein function and cytotoxic aggregation of misfolded proteins that can lead to cell death. To cope with increases in the ER unfolded secretory protein burden, cells have evolved the Unfolded Protein Response (UPR). The UPR is the primary signaling pathway that monitors the state of the ER folding environment. When the unfolded protein burden overwhelms the capacity of the ER quality control machinery, a state termed ER stress, sensor proteins detect accumulation of misfolded peptides and trigger the UPR transcriptional response. The UPR, which is conserved from yeast to mammals, consists of an ensemble of complex signaling pathways that aims at adapting the ER to the new misfolded protein load. To determine how different factors impact the ER folding environment, various tools and assays have been developed. In this review, we discuss recent advances in live cell imaging reporters and model systems that enable researchers to monitor changes in the unfolded secretory protein burden and activation of the UPR and its associated signaling pathways.
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