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1

Foot and mouth disease vaccines and perspectives of their development

100%
Paprocka G., Kesy A.
Biotechnologia
|
1993
|
issue 2
97-104
EN
Progress in techniques of foot and mouth disease vaccine production from conventionally prepared vaccine to synthetic preparates was presented.
2

Efficiency of detecting RNA of FMDV using PCR method according to the starters used for cDNA synthesis

100%
Paprocka G.
|
|
issue 3
114-120
EN
Reverse- transcription polymerase chain reaction has been applied to the detection of FMD viral in samples. Total RNA was extracted with quanidinum thiocyanate-phenol-chloroform method and reversely transcribed using specific or random primers. cDNA was used as a template for the amplification by RT-PCR of the 424 bp of the VP1 coding sequence. Random primers p(dN)6 gave better results.
3

Application of PCR for the detection of FMD viral RNA

80%
Paprocka J., Kesy A., Fitzner A., Niedbalski W., Plucienniczak G.
|
|
issue 4
55-61
EN
The polymerase chain reaction (PCR) method has been applied to the detection of FMD viral RNA in samples taken during the clinical stage of FMD from calf. Total RNA was extracted with guanidinum thiocyanate-phenol-chloroform method and reversety transcribed using AMV-reverse transcriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of the VP1 coding sequence.The amplified fragment of cDNA was cloned in the phagemid pBS(+). The first DNA strand was sequenced and consistently an amino acid sequence was established. Comparison between VP1 amino acid sequence of FMDV types C and earlier described type O was performed.
4

Foot and mouth disease (FMD) - actual diagnostic methods

61%
Paprocka G., Kesy A., Niedbalski W., Fitzner A.
Biotechnologia
|
1997
|
issue 1
131-137
EN
This paper describes the present FMD epizootic situation and virus isolation and identification methods, including new molecular biology diagnostic techniques.
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