Results found: 3

Number of results on page

Search results

Search:
in the keywords: FLUORESCENCE IN SITU HYBRIDIZATION

Sort By:

Limit search:

Supernumerary marker chromosomes characterized by fluorescence in situ hybridization (FISH)

100%

Bocian E., Stankiewicz P., Stanczak H., Obersztyn E., Korniszewski L., Mazurczak T.

Journal of Applied Genetics

1996

vol. 37

issue 3

313-324

Until recently marker chromosomes have presented a difficult diagnostic problem for cytogeneticists as well as for clinicians.Introducing of FISH to cytogenetic analysis has enabled identification of their origin giving possibility to outline specific phenotypic effect of defined marker chromosomes.Nine marker chromosomes were analysed with FISH using centromeric probes, chromosome-specific libraries and unique DNA sequences probes for PWS/AS critical region.The origin from acrocentric chromosomes was established in 6 cases.One marker was a product of maternal 11;22 translocation and two others were pericentromeric regions of chromosome 2 and 4.Among 6 markers, derived from acrocentric chromosomes, 2 consisted of pericentromeric part of chromosome 15, one was identified as mar (21) and in 3 other cases the origin could not be differentiated between chromomsomes including the risk for chromomsomal nondisjunction and trisomy 21 as well as the risk uniparental disomy (UPD) are discussesd.

Cytogenetic markers of Brassica napus L. chromosomes

100%

Hasterok R., Maluszynska J.

Journal of Applied Genetics

2000

vol. 41

issue 1

1-9

Brassica napus has relatively small and poorly differentiated chromosomes. The total length, arm ratio and localisation of 18S-5.8S-26S rRNA genes formed the basis for the preparation of the ideogram of metaphase chromosomes. The morphometric features of the B. napus chromosomes allow for their classification into three morphological groups, but it is difficult to distinguish particular chromosome pairs within the groups. rRNA genes are present in 12 chromosomes of the diploid complement and are located in three chromosomal positions: secondary constrictions, terminal and pericentromeric regions. All rDNA loci at the secondary constriction are active. The signals of in situ hybridisation with rDNA co-localise with CMA positive bands in most of the loci. It was found that rRNA genes are good markers for some B. napus chromosomes, but still more cytogenetic markers are needed for the identification of all chromosome pairs.

Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium/Festuca complex

75%

Humphreys M. W., Thomas H. M., King I. P., Morgan W. G., Meredith M. R., Harper J. A., Humphreys M. O., Bettany M. O., Dalton S. J., James A. R.

issue 3

273-284

Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for 'dissecting' quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.

Refine search results

3 Journal of Applied Genetics

1 2000

1 1997

1 1996

1 Bettany M. O.

1 Bocian E.

1 Dalton S. J.

1 Harper J. A.

1 Hasterok R.

1 Humphreys M. O.

1 Humphreys M. W.

1 James A. R.

1 King I. P.

1 Korniszewski L.

1 Maluszynska J.

1 Mazurczak T.

1 Meredith M. R.

1 Morgan W. G.

1 Obersztyn E.

1 Stanczak H.

1 Stankiewicz P.

1 Thomas H. M.

Search results

Supernumerary marker chromosomes characterized by fluorescence in situ hybridization (FISH)

Cytogenetic markers of Brassica napus L. chromosomes

Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium/Festuca complex