Search results

1

Recrutation and settlement of young flounder (Platichthys flesus) in the western part of the Gulf of Gdansk (Baltic)

100%

Sapota M. R., Kaminska U.

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1998

|

vol. 27

|

issue 3

31-42

EN

On a basis of whole year monthly sampling, the abundance of the flounder in inshore shallow waters of the western part of the Gulf of Gdansk was established. Flounders were observed almost all year. Only in winter, when sea ice forming proc-esses occurred, they disappeared from the investigated zone. The highest abundance of the flounder was noticed in Hel vicinity. No flounders were observed in inshore waters of the Puck Lagoon. Young flounders occurred in the highest number in July (average for all investigated area amounted to 2.81 individuals 100/ m^2). In that month, this-year-born flounders settle in the inshore shallow water zone. The highest biomass was noticed in August (average 4.98 g/100 m^2) when older bigger flounders more frequently inhabited inshore zone. Body proportions of young flounders during their growth in inshore shallow waters of the Gulf of Gdansk are stable. All measured distances were increasing linearly with the growth of flounder length.

2

Turner syndrome mosaicism: an unusual case with a de novo large dicentric marker chromosome: mos 45,X/46,X, ter rea(X;X)(p22.3;p22.3)

100%

Nucaro A. L., Melis P., Casini M. R., Rossino R., Cau M., Melis M. A., Loche S.

Journal of Applied Genetics

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2008

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vol. 49

|

issue 3

301-303

EN

X/X translocations are quite rare in humans. The effect of this anomaly on the phenotype is variable and depends on the amount of deleted material and whether the chromosomes are joined by their long or short arms. We report an unusual case of Turner syndrome mosaicism in a 16-year-old girl, who was referred to our Institute for primary amenorrhoea associated with short stature. Endocrine evaluation revealed hypergonadotropic hypogonadism, which required a study of the karyotype. Cytogenetic analysis, performed on peripheral blood leucocytes, showed a mos 45,X/46,X,ter rea (X;X)(p22.3;p22.3) de novo karyotype. The prevalent cell line was 45,X (90% cells). A second cell line (10% cells) showed a very large marker chromosome, similar to a large metacentric chromosome. FISH (fluorescent in situ hybridisation) and molecular analysis revealed that the marker chromosome was dicentric and totally derived from the paternal X chromosome.

3

Infections factors isolated from fish caught in the Gulf of Gdansk and the Vistula lagoon in 1991-1994

100%

Grawinski E., Dabrowski J., Potajallo U.

|

1998

|

vol. 27

|

issue 4

59-75

EN

The data presents bacteriological virusological investigations results carried out in 1991-1994 in the Gulf of Gdansk and the Vistula Lagoon. The infectious factors study included an identification of bacteria species and orders that are pathogenic to fish and man and also a test on the presence of viruses constituting a potential hazard to man's health. The investigations were a complementary study to the ones carried out in 1981.

4

Growth of the young flounder (Platichthys flesus) in inshore shallow waters of the Gulf of Gdansk

100%

Sapota M. R., Mudrak S.

|

1998

|

vol. 27

|

issue 3

19-29

EN

The age and rate of growth of the flounder from inshore shallow waters of the Gulf of Gdansk (from shore to 1-m depth) have been investigated. Mainly the flounder from 0 and 1-year age group exists in this zone. Flounders from 0-year age group enter shallow waters of the Gulf of Gdansk in June or July. They stay in this area till the end of the year. Flounders from 1-year age group are represented in the investigated zone all year. Bigger individuals from this group migrate into deeper waters.

5

B chromosomes in two fish species genus Rhamdia (Siluriformes, Pimelodidae)

100%

Fenocchio A. S., Bertolo L. A. C., Takahashi C. S., Camacho J. P. M.

Folia Biologica

|

2000

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vol. 48

|

issue 3-4

105-109

EN

Specimens belonging to two fish species genus Rhamdia were cytogenetically analysed from seven localities in Brazil and Argentina. In addition to the 58 chromosomes of the basic karyotype, one to five metacentric B chromosomes were observed carrying conspicuous heterochromatic blocks on the distal regions of both chromosome arms. These B chromosomes are mitotically stable and, in the two best-sampled populations in R. hilarii (Lobo and 29 reservoirs), they showed frequency distributions fitting a binomial distribution, though Bs were more frequent in the latter. The presence of B chromosomes with the same appearance in R. quelen suggests an ancient origin for these B chromosomes, presumably prior to speciation from a common ancestor.

6

Additional chromosome in a child as a result of a balanced reciprocal translocation t(12;18)(p13;q12) in his mother's karyotype

100%

Lassota M., Przelozna B., Plodzien M., Bugno M., Wnuk M., Kotylak Z., Slota E.

|

EN

In this case report we present a child with an additional chromosome in the karyotype. The karyotypes of the boy and his parents were analyzed by use of a conventional banding technique (GTG) and fluorescence in situ hybridization (FISH). Probes painting whole chromosomes 12 and 18 were used in FISH. Cytogenetic examination of the parents revealed that his mother was carrying balanced reciprocal translocation between chromosomes 12 and 18. Her karyotype was described as 46,XX,t(12;18)(p13;q12). Father's karyotype was normal, described as 46,XY. The boy's karyotype was defined as 47,XY,+der(18)t(12;18)(p13;q12). The additional chromosome appeared probably due to 3:1 meiotic disjunction of the maternal balanced translocation, known as tertiary trisomy. The mother displayed a normal phenotype and delivered earlier a healthy child. However, the boy with the unbalanced karyotype shows multiple congenital abnormalities.

7

Seasonal and daily distribution of commercial and non-commercial fish in shallow inshore waters of the Gulf of Gdansk

100%

Lizinska A.

Oceanological Studies

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2002

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vol. 31

|

issue 1-2

31-42

EN

Seasonal and daily changes in the fish community and variations between commercial and non-commercial fish abundance were monitored in the inshore waters of the western part of the Gulf of Gdask (the Sopot territory). Samples were collected from December 1998 to December 1999, though monthly samplings, and supplemented by 24-hour investigations taken every three months, using a two-meter wide trawl. Among the 16 species collected, 5 were commercial and 11 were non-commercial. Juvenile forms of herring and flounder dominated the commercial fish; the highest catches were in March (89 ind. 100 m-2). Among the non-commercial fish, two species of Gobiidae (sand and common goby), and three-spine stickleback were most frequently caught. During the 24hour observations, commercial fish were noticed mostly at night. The investigated area is an important zone for both juvenile commercial fish and some ecologically important uncomercial species.

8

Total mercury content in fish from the Gulf of Gdansk

100%

Falandysz J., Piotrowska M., Wlodarczyk J., Chwir A., Marcinowicz^A., Marcinowicz A.

|

issue 67/10

59-64

EN

Concentratin of total mercury was determined in muscle tissue of cod Gadus morhua, herring Clupea harengus, eel Anguilla anguilla, calpout Zoarces viviparus, perch Perca fluvitalis, pikeperch Stizostedion lucioperca, sand eel Hyperoplus lanceolatus, round goby Negobius melanostomus, brown trout Salmo trutta, trout Salmo gairdneri, flounder Platychtis flesus, turbot Psetta mazima, smelt Osmerus eperlanus and sculpin Cottus scorpius caught in the Gulf of Gdansk in 1986-1993.The measurements of mercury concentrations were performed by the method of cold vapour atomic absorption spectrmetry (CV-AAS), after wet digestion of samples with concentrated nitric acid.The highest mean concentration of mercury was found in eelpout, the lowest one in pikeperch.The possitive linear correlations has been found between the body length and mercury concentration in perch, eelpout, sand launce and flounder.

9

Karyotypic characterization and NOR analysis by different banding techniques of Rhamdia quelen from the first plateau of the Iguacu River (Brazil)

100%

Fenocchio A. S., Swarca A. C., Cestari M. M., Dias A. L.

Folia Biologica

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2003

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vol. 51

|

issue 3-4

219-222

EN

A population of R. quelen from the first plateau of the Iguacu River (Parana State, Brazil) was analyzed cytogenetically. A diploid set of 58 chromosomes was constituted by 32 M, 16 SM, 6 ST and 4 A (FN=116). In one individual a 2n=59 karyotype was determined due to the presence of one additional metacentric heterochromatic chromosome. NORs, detected by AgNO3 and CMA3 staining as well as fluorescence in situ hybridization with an 18S rDNA probe, are located at a terminal position on the short arms of a ST chromosome pair. C-banding marks the NORs and other weak bands distributed on telomeric regions of some chromosomes. These results give evidence that Rhamdia constitute, in terms of karyotypic macrostructure, a conserved group and support the idea that several synonymous species may be included in this genus.

10

Influence of environmental factors on myxosporean infestation of Black Sea fish in coastal waters

100%

Yurakhno V. M.

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vol. 26

|

issue 1

75-85

EN

The myxosporean fauna of the Black Sea is predominantly marine with a few brackish and fresh-water species. Seasonal changes in water temperature influence the life-cycle of Black Sea myxosporeans primarily through the biology of the host. This is because the mass formation of spores in Myxosporea takes place when hosts form the largest shoals. The high saturation of the water in Tendrovsky Bay with hydrogen sulphide accounts for the mass mortality of fish, but and at the same time for the fact that many fish species were found to be free of parasites. Myxosporeans occur practically at any depth where their hosts can live.

11

Isolation and chromosomal distribution of a novel Ty1-copia-like sequence from Secale, which enables identification of wheat?Secale africanum introgression lines

100%

Jia J., Yang Z., Li G., Liu C., Lei M., Zhang T., Zhou J., Ren Z.

Journal of Applied Genetics

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2009

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vol. 50

|

issue 1

25-28

EN

A repetitive sequence of 411 bp, named pSaO5411, was identified in the Secale africanum genome (Ra) by random amplified polymorphic DNA (RAPD) analysis of wheat and wheat?S. africanum amphiploids. GenBank BLAST search revealed that the sequence of pSaO5411 was highly homologous to a part of a Ty1-copia retrotransposon. Fluorescence in situ hybridization (FISH) analyses indicated that pSaO5411 was significantly hybridized to S. africanum chromosomes of a wheat?S. africanum amphiploid, and it was dispersed along the Secale chromosome arms except the terminal regions. Basing on the sequence of pSaO5411, a pair of sequence-characterized amplified region (SCAR) primers were designed, and the resultant SCAR marker was able to target both cultivated rye and the wild Secale species, which also enabled to identify effectively the S. africanum chromatin introduced into the wheat genome.

12

Carotenoid content in Lota lota (L.) individuals in various biological activity periods

100%

Czeczuga B., Czeczuga-Semeniuk E.

Folia Biologica

|

1999

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vol. 47

|

issue 3-4

67-72

EN

The authors investigated the carotenoid content in the particular parts of Lota lota in summer, autumn, and winter, i.e. when burbots exhibit the lowest and highest activity. By means of columnar and thin-layer chromatography, the following carotenoids were found to be presenct: a-carotene, b-carotene, e-carotene, b-cryptoxanthin, neothxanthin, lutein, 3?-epi- lutein, zeaxanthin, tunaxanthin, antheraxanthin, lutein epoxide, echinenone, 3?-hydroxy- echinenone, idoxanthin, canthaxanthin, a-doradexanthin, b-doradexanthin, astaxanthin, diatoxanthin, parasiloxanthin, monadoxanthin, 7,8-dihydroparasiloxanthin, mutatoxanthin and rhodoxanthin. In the Lota lota individuals examined, the content of carotenoids was found to differ in winter and summer. The total carotenoid content ranged from 0.067 (gonads of males) of to 6.095 ?g g-1 wet weight (fins of males from December).

13

Chromosome banding studies by replication and restriction enzyme treatment in vendace (Coregonus albula) (Salmonidae, Salmoniformes)

88%

Jankun M., Ocalewicz K., Mochol M.

Folia Biologica

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2004

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vol. 52

|

issue 1-2

47-51

EN

Chromosome banding studies were performed in vendace, Coregonus albula. Original data on distribution of early and late replication regions, restriction sites (AluI, DdeI, HinfI and HaeIII) on chromosomes in this coregonid fish have been used to analyse karyotype heterochromatin differentiation. Heterochromatic bands (C-positive and not digested by restriction enzymes) have been identified as late replicating regions. Extra bands produced by the applied methods have permitted the identification of several homologous pairs. The centromeres were differentially digested by the restriction enzymes. The studied population seems to be homogenic regarding karyotype characteristics.

14

Possible ancient origin of heterochromatic JNK sequences in chromosomes 2R of Secale vavilovii Grossh

88%

Achrem M., Rogalska S. M., Kalinka A.

Journal of Applied Genetics

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2010

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vol. 51

|

issue 1

1-8

EN

Employing FISH analysis as well as BLAST and CUSTAL W (1.82) programs, we investigated types of DNA nucleotide sequences building an additional heterochromatic band in 2R chromosomes of 3 lines of Secale vavilovii Grossh. The probes used in FISH analysis were designed based on the reverse transcriptase sequence of Ty1-copia and Ty3-gypsy retrotransposons and the 5S rRNA gene sequence. No hybridization signals from the reverse transcriptase probes were observed in the chromosome region where the additional band occurs. On the other hand, signals were observed after hybridization with the 5S rDNA probe, clearly suggesting the presence of that type of sequences in the analyzed heterochromatin band. Using BLAST and CUSTAL W programs, we revealed high similarity of the JNK1 sequence to the 5S rRNA gene from Hordeum chilense (HCH1016, HCH1018, 88%) and to a fragment of the 5S rRNA sequence of H. marinum (HMAR003, 97%). In addition, the same fragment of JNK1 was shown to be very similar to the part of the Angela retrotransposon (92%) as well as to the SNAC 426K20-1 transposon (89%) belonging to CACTA family, both from Triticum monococcum, and to Zingeria biebersteiniana pericentromeric sequences (78%). The similarity of JNK1 to those sequences may be accidental or the JNK1 may represent an ancient mobile genetic element that caught the 5S rRNA sequence. During the evolution those sequences might have been accumulated in the particular region on the 2R chromosome. Our results suggest that the additional heterochromatin band in chromosomes 2R of S. vavilovii is a collection of defective genes and/or mobile genetic elements.

15

Tools of the trade: diagnostics and research in domestic animal cytogenetics

88%

Iannuzzi L., Berardino_Di D.

Journal of Applied Genetics

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2008

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vol. 49

|

issue 4

357-366

EN

This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase chromosomes for mapping purposes. Each section includes historical information, basic principles for the given technique, its primary use in veterinary cytogenetics, and major limitations. Supplementary material (protocols and chemicals used) are available on our website. Even though these techniques mainly refer to the Bovidae, they can be easily extended and adapted to members of other taxa.

16

Acoustic research on Baltic fish behaviour in relation to variable environmental factors

88%

Orlowski A.

|

issue 2

19-32

EN

The paper describes new methods and approaches to treating acoustic, biological and environmental data collected during surveys. Examples of their applications are given. The aim of all the methods presented is to correlate acoustic, biological and environmental data with the task of estimating and mathematically describing (modelling) interactions between environment, fish, and its relevant acoustic response. The methods are intended for marine ecologists and fisheries acous-ticians.

17

Spatial (depth dependent) and temporal distribution of fish in sandy eulittoral of the tip of the Hel Peninsula (the Gulf of Gdansk - Baltic)

75%

Sapota M. R.

Oceanological Studies

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2001

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vol. 30

|

issue 3-4

77-89

EN

Presence of fish from 10 species was confirmed in shallow close to shore waters on the tip of the Hel Peninsula, in period from early spring to late autumn. Zone from shore to 5 meter depth was investigated. The highest number and biomass were noticed in summer on 3 meter depth. As general biodiversity taking into account number increases with increasing depth, whereas biodiversity taking into account biomass is not so depth dependent. Flounder is the absolute dominant at all depths in investigated region. Common goby and three spined stickleback are two other significant species. Comparison of data from all investigated depths shows that sampling in the most close to shore zone (1 meter depth) let well describe fish community of near shore shallow waters in investigated area.

18

Effects of light and darkness on cell deaths in damaged retinal ganglion cells of the carp retina

75%

Dezawa M., Mo X., Oshitari T., Takano M., Meyer-Rochow V. B., Sawada H., Eguchi E.

Acta Neurobiologiae Experimentalis

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2001

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vol. 61

|

issue 2

85-91

EN

Effects of light and darkness on the apoptosis of retinal ganglion cells (RGCs) in young carp were measured by TUNEL method after transection of the optic nerve. Following the operation, the fish were kept under one of four regimens; constant darkness (DD), constant light (LL), 12 hr light and 12 hr dark (LD) and 3 hr of flickering light followed by 21 hr in the dark (FL). On day 3, the highest ratio of apoptotic RGCs was seen under conditions of DD, followed by LL, LD, and FL. On day 6, the percentages of apoptotic RGCs were lower under every experimental condition than what they had been earlier on day 3, but the same ranking order was maintained. Immunohistochemically it could be shown that phosphorylated ERKs were more intensively localized in FL rather than DD retinas. The results suggest that illumination regimens, and in particular cyclic diurnal light/dark changes, have an influence on the degree of apoptosis of damaged RGCs, and that inhibition of apoptosis is correlated with the higher expression of phosphorylated ERKs.

19

Multicolour FISH in an analysis of chromosome aberrations induced by N-nitroso-N-methylurea and maleic hydrazide in barley cells

75%

Juchimiuk J., Hering B., Maluszynska J.

Journal of Applied Genetics

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2007

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vol. 48

|

issue 2

99-106

EN

The present study is a rare example of a detailed characterization of chromosomal aberrations by identification of individual chromosomes (or chromosome arms) involved in their formation in plant cells by using fluorescent in situ hybridization (FISH). In addition, the first application of more than 2 DNA probes in FISH experiments in order to analyse chromosomal aberrations in plant cells is presented. Simultaneous FISH with 5S and 25S rDNA and, after reprobing of preparations, telomeric and centromeric DNA sequences as probes, were used to compare the cytogenetic effects of 2 chemical mutagens: N-nitroso-N-methylurea (MNU) and maleic hydrazide (MH) on root tip meristem cells of Hordeum vulgare (2n = 14). The micronucleus (MN) test combined with FISH allowed the quantitative analysis of the involvement of specific chromosome fragments in micronuclei formation and thus enabled the possible origin of mutagen-induced micronuclei to be explained. Terminal deletions were most frequently caused by MH and MNU. The analysis of the frequency of micronuclei with signals of the investigated DNA probes showed differences between the frequency of MH- and MNU-induced micronuclei with specific signals. The micronuclei with 2 signals, telomeric DNA and rDNA (5S and/or 25S rDNA), were the most frequently observed in the case of both mutagens, but with a higher frequency after treatment with MH (46%) than MNU (37%). Also, 10% of MH-induced micronuclei were characterized by the presence of only telomere DNA sequences, whereas there were almost 3-fold more in the case of MNU-induced micronuclei (28%). Additionally, by using FISH with the same probes, an attempt was made to identify the origin of chromosome fragments in mitotic anaphase.

20

Efficiency of municipal wastewater treatment with membrane bioreactor

75%

Domańska M., Boral A., Hamal K., Kuśnierz M., Łomotowski J., Płaza-Ożóg P.

Journal of Water and Land Development

|

2019

|

issue 41

Refine search results

Recrutation and settlement of young flounder (Platichthys flesus) in the western part of the Gulf of Gdansk (Baltic)

Turner syndrome mosaicism: an unusual case with a de novo large dicentric marker chromosome: mos 45,X/46,X, ter rea(X;X)(p22.3;p22.3)

Infections factors isolated from fish caught in the Gulf of Gdansk and the Vistula lagoon in 1991-1994

Growth of the young flounder (Platichthys flesus) in inshore shallow waters of the Gulf of Gdansk

B chromosomes in two fish species genus Rhamdia (Siluriformes, Pimelodidae)

Additional chromosome in a child as a result of a balanced reciprocal translocation t(12;18)(p13;q12) in his mother's karyotype

Seasonal and daily distribution of commercial and non-commercial fish in shallow inshore waters of the Gulf of Gdansk

Total mercury content in fish from the Gulf of Gdansk

Karyotypic characterization and NOR analysis by different banding techniques of Rhamdia quelen from the first plateau of the Iguacu River (Brazil)

Influence of environmental factors on myxosporean infestation of Black Sea fish in coastal waters

Isolation and chromosomal distribution of a novel Ty1-copia-like sequence from Secale, which enables identification of wheat?Secale africanum introgression lines

Carotenoid content in Lota lota (L.) individuals in various biological activity periods

Chromosome banding studies by replication and restriction enzyme treatment in vendace (Coregonus albula) (Salmonidae, Salmoniformes)

Possible ancient origin of heterochromatic JNK sequences in chromosomes 2R of Secale vavilovii Grossh

Tools of the trade: diagnostics and research in domestic animal cytogenetics

Acoustic research on Baltic fish behaviour in relation to variable environmental factors

Spatial (depth dependent) and temporal distribution of fish in sandy eulittoral of the tip of the Hel Peninsula (the Gulf of Gdansk - Baltic)

Effects of light and darkness on cell deaths in damaged retinal ganglion cells of the carp retina

Multicolour FISH in an analysis of chromosome aberrations induced by N-nitroso-N-methylurea and maleic hydrazide in barley cells

Efficiency of municipal wastewater treatment with membrane bioreactor