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Neurons derived from PC12 cells have the potential to develop synapses with primary neurons from rat cortex

100%

Zhou T., Xu B., Que H., Lin Q., Lv S., Liu S.

Acta Neurobiologiae Experimentalis

2006

vol. 66

issue 2

105-112

Neuron transplantation is considered to be a promising therapeutic method to replace functions lost due to central nervous system (CNS) damage. However, little is known about the extent to which implanted neuron-like cells can develop into mature neurons and acquire essential properties, and especially form synapses with host neurons. In this investigation we seeded PC12 cells labeled with GFP into primary cultured neurons isolated from rat cerebral cortex to build a co-culture system, and then induced the PC12 cells to differentiate into neuron-like cells with NGF. Seven days later, we observed the relationship between the PC12-derived neurons and primary neurons using FM1-43 imaging and immunoelectron microscopy, and found that GFP-labeled neurons could form typical synapses with host primary neurons. These observations showed that immigrant neurons differentiated from PC12 cells could develop into mature neurons and could form intercellular contacts with host neurons. Both the immigrant and host neurons could construct neuronal networks in vitro.

Characterization and plasticity of the double synapse spines in the barrel cortex of the mouse

88%

Jasinska M., Siucinska E., Glazewski S., Pyza E., Kossut M.

Acta Neurobiologiae Experimentalis

2006

vol. 66

issue 2

99-104

The somatosensory barrel cortex of rodents and its afferent pathway from the facial vibrissae is a very useful model for studying neuronal plasticity. Dendritic spines are the most labile elements of synaptic circuitry and the most likely substrate of experience ? dependent alterations in neuronal circuits in cerebral cortex. We characterized morphologically and numerically a specific population of spines, i.e. double synapse spines, which have two different inputs ? one excitatory and the other inhibitory, in the B2 barrel of mouse somatosensory cortex. We also described changes in morphology of double synapse spines induced by classical conditioning in which stimulation of vibrissae was paired with a tail shock. The analysis was carried out by means of serial EM micrograph reconstruction. We showed that double spines account for about 10% of all analyzed spines. The morphology of a typical double synapse spine is similar to the morphology of single synapse spine and both consist of two parts ? a large head and a narrow, long neck. Excitatory synapses are preferentially located on the head of double synapse spines and inhibitory synapses are usually located on the neck of these spines. The length of the double synapse spine neck decreases and the cross-section area of the spine neck increases significantly as a result of sensory conditioning.

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2 Acta Neurobiologiae Experimentalis

2 2006

1 Glazewski S.

1 Jasinska M.

1 Kossut M.

1 Lin Q.

1 Liu S.

1 Lv S.

1 Pyza E.

1 Que H.

1 Siucinska E.

1 Xu B.

1 Zhou T.

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Neurons derived from PC12 cells have the potential to develop synapses with primary neurons from rat cortex

Characterization and plasticity of the double synapse spines in the barrel cortex of the mouse