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1

Cytogenetic characterization of Geophagus brasiliensis and two species of Gymnogeophagus (Cichlidae: Geophaginae) from Guaiba Lake, RS, Brazil

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Bettin_ P. L., Giuliano-Caetano L., Dias A. L.
Folia Biologica
|
2010
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vol. 58
|
issue 1-2
29-34
EN
The karyotypes of three species of fish of the Cichlidae family from the Forqueta river and several locations in Gua?ba lake/RS (Brazil) were analyzed. All species presented 2n=48, while Gymnogeophagus gymnogenys showed two karyotypic formulae: 4m+44st-a with FN=52 and 6m+42st-a with FN=54. Gymnogeophagus labiatus presented 4m+4sm+40st-a and FN=56 and Geophagus brasiliensis 4sm+44st-a and FN=52. Simple NORs were found in all species with the exception of a population of G. gymnogenys from Saco da Alemoa/Barra do Ribeiro. CMA3 staining revealed NOR sites, while DAPI staining was negative and heterochromatin was limited to pericentromeric regions and associated to NORs, except in G. labiatus. The data show a conserved pattern in Geophagus brasiliensis and karyotype variation in the species of Gymnogeophagus.
2

Diversity of diploid androgenic Brussels sprout plants of R0 and R1 generations

100%
Kaminski P., Dyki B., Krzyzanowska D., Gorecka K.
Journal of Applied Genetics
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2005
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vol. 46
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issue 1
25-33
EN
Androgenic Brussels sprout plants were produced by the use of anther culture from the donor cultivar 'Philemon F1'. A total of 96 plants obtained from 20 androgenic R0 genotypes assigned as diploids were evaluated both in the generative and vegetative stage, in respect of their morphological characters: mean plant height; leaf size, colour and waxiness; leaf blade shape, blistering and attitude; number of sprouts; as well as their self-incompatibility and fertility. Androgenic R0 plants derived from each of the 20 embryos were highly diversified and differed from the donor in one or more morphological traits in the vegetative stage. Evaluated populations also varied in fertility and self-incompatibility. Six androgenic genotypes that set a sufficient amount of seeds of the R1 generation and 'Philemon F1' were evaluated in the field in respect of plant height, total and marketable yield per plot, shape of stem with sprouts, shape and density of sprouts, and spacing between sprouts. Only four diploid R0 and R1 populations may have some value for further breeding, as they are characterised by good vigour, high or medium ability for sprout generation, and sufficient fertility.
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