Dextransucrase catalyses the synthesis of a-D-glucopyranosyl homopolymers and oligomers from sucrose as a substrate. This paper is a review of reports on the mechanism of dextran and oligosaccharides synthesis. The presence of a low molecular acceptor dextran (3-20 kDa) or low molecular acceptor sugars in reaction mixtures enables obtaining of dextran with molecular weight in a range from 30 to 80 kDa or oligosaccharides with low d.p., which have gained increased nutritional interest (prebiotics). In these reactions, the hydrolytic enzyme, i.e. dextranase, may be involved together with dextransucrase instead of acceptors.
Dextran is a bacterial polysaccharide composed almost exlusively of the monomeric unit a-1,6-glucopyranose linked mainly by a-1,6-bonds. Enzymatic hydrolysis of dextran is carried out with the enzyme dextranase. This paper is a review of world-wide literature concerning properties and production of dextran and dextranase. The important part of the article is the discussion about their significant role and real or potential applications in industry and medicine.
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