Search results

1

Characteristics and results of treatment of patients treated surgically with colorectal cancer in old and senile age

100%

Banaszkiewicz Z., Zwoliński T., Tojek K., Jarmocik P., Jawień A.

Polish Journal of Surgery

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2018

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vol. 90(1)

1-6

EN

Colorectal cancer (CC) in Poland is the type of cancer with the highest dynamics of disease growth and is epidemiologically related to age. The analysis involved 353 patients operated on due to CC in senile and old age and compared with younger patients. It was found that people at this age are more often diagnosed with CC They were more often women, the patients did not differ in the stage of cancer, while they were significantly more often qualified for surgery due to urgent indications. In patients with colonic cancer, the resectability and radicality of the procedures in comparison with patients with rectal cancer was significantly higher, while there were more complications and deaths in the 30-day follow-up in this group. The overall survival in senile and old age was significantly worse. In the first year of follow-up after surgical treatment of patients in this group, complications and deaths were more frequently observed. However, in patients who survived 12 months after the operation, the overall survival rate did not significantly differ.

2

Association of EGF and p53 gene polymorphisms and colorectal cancer risk in the Slovak population

80%

Mahmood S., Sivoňová M., Matáková T., Dobrota D., Wsólová L., Dzian A., Mištuna D.

Open Medicine

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2014

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vol. 9

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issue 3

405-416

EN

During the transformation process single nucleotide polymorphisms (SNPs) of key genes, such as p53 Arg72Pro or EGF A61G, may mediate various cellular processes. These variants may be associated with colorectal cancer risk (CRC), but conflicting findings have been reported. The purpose of this study was to determine the association of the SNPs in 5′ UTR of EGF A61G and p53 Arg72Pro and CRC in the Slovak population. The present case-control study was carried out in 173 confirmed CRC patients and 303 healthy subjects. Genotyping was performed by PCR-RFLP methods. Significant association was observed between age and CRC risk (p=0.001). Lower CRC risk was seen in younger patients carrying genotype p53 Arg72Pro (0.14; 95% CI 0.02–0.99, p=0.049). Gender-stratified analysis showed a significant inverse association of the polymorphism EGF G61G with CRC risk (0.48; 95% CI 0.2–0.9, p=0.04) only in male patients. Tumour site genotype distribution revealed that female patients with localized colon cancer were significantly associated with p53 Pro72Pro genotype (4.0; 95% CI 1.27–12.7, p=0.04) whereas the cancer of rectosigmoid junction was associated with the EGF G61G genotype (4.5; 95% CI 1.2–16.97, p=0.02). Combination of p53 Arg72Pro or EGF A61G polymorphisms were not associated with CRC risk by using logistic regression.

3

Sentinel lymph node in colorectal cancer - 5 years follow up

80%

Zielinski J., Jaworski R., Kabata P., Rzepko R., Kruszewski W., Jaskiewicz J.

Open Medicine

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2011

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vol. 6

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issue 3

271-278

EN

To assess the impact of micrometastases in sentinel and non-sentinel lymph nodes on long-term survival rates of patients treated for colorectal cancer (CRC). Data of 57 patients diagnosed with CRC and treated in the Department of Surgical Oncology in Gdansk in the years 2002–2006 were retrospectively analyzed. Clinico-histopathological data were analyzed using chi-square tests. The effect on long-time survival rates was analyzed using Kaplan-Meier survival probability estimates. Identification of the SLN was performed using the blue dye staining method. All regional lymph nodes were subject to standard histopathological examination. Additionally in 32(56.14%) patients whose nodes were found negative for metastases on standard staining further immunohistochemical analyses were performed. In the analyzed group SLNB was performed in 42(73.7%) patients with colon cancer and in 15(26.3%) with rectal cancer. Identification of the SLN was possible in 45(78.9%) patients. The sensitivity of SLNB was 33%. False negatives were found in 66%. SLNB is a feasible method in CRC patients. We presume that lack of micrometastases in the SLN and non-SLN cannot be regarded as a prognostic factor.

4

Incidence of colorectal cancer in Lower Silesia (Poland) between 1984 and 2003 - trends and perspectives

70%

Bębenek M., Pudełko M., Błaszczyk J.

Open Medicine

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2008

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vol. 3

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issue 1

83-87

EN

The purpose of the present study was to demonstrate the epidemiological characteristics of colorectal cancers (CRCs) diagnosed in Lower Silesia between 1984 and 2003. Data from the Lower Silesian Cancer Registry on the incidence of CRC in the Lower Silesian province were subjected to analysis. The age adjusted incidence of CRC in both genders increased markedly. A higher relative increase of incidence was recorded for colon cancer. The age-specific incidence of either colon or rectal cancer increased markedly with age in both genders. No constant time-trend toward the earlier diagnosis of CRC was noted in the period studied. Although the number of CRC surgeries performed at the Regional Comprehensive Cancer Center in Wroclaw has increased over time, most of the cases that were diagnosed were treated outside that reference center. If an intensive increase in new case numbers, demonstrated in both the locations by 2003, is not moderated, the incidence of colorectal malignancies in Lower Silesia would become one of the highest in Europe.

5

Altered membrane amino acids composition in human colorectal cancer tissue

70%

Szachowicz-Petelska B., Sulkowski S., Figaszewski Z.

Open Chemistry

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2012

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vol. 10

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issue 4

1245-1252

EN

Cancer transformation is characterized by changes in cell metabolism, which can alter the structure and function of cell membrane components, including integral membrane proteins. Qualitative and quantitative estimations of integral membrane protein are necessary for studies aimed at understanding their modifications under pathological conditions. Herein, we used a high-performance liquid chromatography (HPLC)-based approach that involved selective hydrolysis of isolated tissue cell membrane proteins to peptides, resolution by chromatography and determination of the amino acid content (phenylalanine (Phe), tyrosine (Tyr), cysteine (Cys) and lysine (Lys)) in individual peptides. The results demonstrate decrease in peptide levels and their amino acids content in integral membrane proteins in human colorectal cancer tissue. Therefore, cancer transformation causes a decrease in the levels of integral membrane proteins, which may in turn lead to an increase in the levels of other charged molecules on the cell surface, such as phospholipids. It might lead to the reconstruction and functional rearrangement of the cell membrane, for example: the permeability, electric properties, fluidity etc. [...]

6

ANTITUMOR EFFECTS OF VANILLIN BASED CHALCONE ANALOGUES IN VITRO

70%

Luković J., Mitrović M., Popović S., Milosavljević Z., Stanojević-Pirković M., Anđelković M., Zelen I., Šorak M., Muškinja J., Ratković Z., Nikolić I.

Acta Poloniae Pharmaceutica - Drug Research

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2020

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vol. 77

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issue 1

57-67

EN

Chalcones, as a large group of organic compounds, are widely implemented in various types of anti-cancer therapeutics. These plant metabolites are present in fruits, vegetables, spices, and have anti-tumor, anti-inflammation, immunomodulation, antibacterial and anti-oxidative activities, as well as many other pharmacological and biological effects. The aim of the present study was to investigate cytotoxic effects, type of cell death and mechanism of action of the newly synthesized vanillin based chalcone analogues, (CH1) and (CH2) on human colon cancer HCT-116 and noncancerous (control) MRC-5 cell lines. In order to compare effects of vanillin based chalcone analogues on investigated cell lines, as reference substances cisplatin (cisPt) and dehydrozingerone (DHZ) were used. Investigation of antitumor effect of chalcone analogues on HCT-116 cells was carried out by three methods MTT assay, flow cytometry and immunofluorescence analysis. The result of our investigation indicated that newly synthesized vanillin based chalcone analogues expressed powerful antitumor effect on cancer cells (HCT-116 cell line), while their effect on healthy cells (MRC-5 cell line) was not statistically significant. Vanillin based chalcone analogues caused overexpression and activation of mitochondrial Bax protein and caspase-3 in HCT-116 cells, indicating that their mechanism of antitumor action was mediated through activation of inner apoptotic pathway. These results indicate possible usefulness of CH1 and CH2 in antitumor therapy whether through its direct cytotoxic effect or as adjuvant therapy. Our results indicate possible usefulness of CH1 and CH2 vanillin based chalcone analogues in antitumor therapy.

7

Zastosowanie probiotyków w profilaktyce oraz leczeniu raka żołądka i raka jelita grubego

51%

Kaźmierczak- Siedlecka K. L., Makarewicz W.

Farmacja Polska

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2020

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vol. 76

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issue 2

118-124

EN

The composition of gut microbiota depends on many factors, such as age, life style (eating habits and the level of physical activity), pharmacological treatment (antibiotics, side effects of anti-cancer therapy) as well as surgical procedures. The gut microbiota is involved in carcinogenesis process. Furthermore, gut dysbiosis is described as qualitative and quantitative alterations in gut microbiota and it is observed in cancers. For instance, in patients with colorectal cancer the increased amount of Fusobacterium nucleatum, Bacteroides fragilis, Enterococcus faecalis, Streptococcus bovis as well as Peptostreptococcus anaerobius is noted. It was confirmed that amount of several specific bacteria, such as Lactobacillus, Escherichia-Shigella, Nitrospirae, Burkholderia fungorum and Lachnospiraceae, is increased in patients with gastric cancer. However, the major carcinogen involved in gastric carcinogenesis is Helicobacter pylori; it causes mucosa inflammation, mucosa atrophy, and as a consequence development of gastric cancer. Nowadays, there are several therapeutic methods, which may be used to alter the composition and the activity of gut microbiota. They include administration of probiotic strains, prebiotics, and synbiotics. Probiotics can be used to prevent the development of gastric and colorectal cancer, which was shown in many in vivo and in vitro studies. According to the most recent trials, probiotics reduce the incidence of diarrhoea associated with enteral nutrition. Probiotic strains may also be used as a supportive therapy in treatment of Helicobacter pylori infection. Notwithstanding, they can play a supportive role in standard eradication treatment due to reduction of adverse events of antibiotics. Probiotics decrease the incidence of infections in postooperative period, the frequency of abdominal pain, and radiation-induced diarrhoea. To sum up, probiotics may be used to prevent the development of cancer and they may significantly improve the efficiency of standard anti-cancer therapy.

PL

Mikrobiota przewodu pokarmowego jest modyfikowana przez wiele czynników, w tym styl życia, leczenie farmakologiczne oraz zabiegi chirurgiczne. Dysbioza jelitowa, czyli zaburzenia w składzie i aktywności mikrobioty, może wystąpić w przebiegu chorób nowotworowych. U pacjentów z rakiem jelita grubego obserwuje się zwiększone ilości bakterii – Fusobacterium nucleatum, Bacteroides fragilis, Enterococcus faecalis, Streptococcus bovis oraz Peptostreptococcus anaerobius. Największym karcynogenem raka żołądka jest Helicobacter pylori. Bakteria ta powoduje zapalenie błony śluzowej żołądka prowadząc do jej atrofii, a następnie do rozwinięcia nowotworu. Obecnie znanych jest kilka metod terapeutycznych modyfikujących mikrobiotę przewodu pokarmowego, w tym podaż szczepów probiotycznych, prebiotyków oraz synbiotyków. Probiotyki mogą być stosowane w profilaktyce oraz leczeniu raka żołądka i jelita grubego, co zostało potwierdzone w badaniach in vivo oraz in vitro. Według najnowszych doniesień, probiotyki są skuteczne w zmniejszeniu częstości występowania biegunki będącej skutkiem ubocznym żywienia enteralnego. Dotychczasowe badania potwierdzają także, że szczepy probiotyczne nie mogą być stosowane jako jedyny czynnik eradykacyjny Helicobacter pylori, ale stanowią terapię uzupełniającą podczas standardowego leczenia oraz redukują działania niepożądane antybiotykoterapii. Z kolei u chorych z rakiem jelita grubego probiotyki zmniejszają ryzyko rozwinięcia infekcji pooperacyjnych, bólów brzucha i biegunki związanej z radioterapią. Podsumowując, należy podkreślić, że probiotyki mają zastosowanie na etapie profilaktyki chorób nowotworowych, a także mogą znacząco poprawić wyniki standardowego leczenia przeciwnowotworowego.

Refine search results

Characteristics and results of treatment of patients treated surgically with colorectal cancer in old and senile age

Association of EGF and p53 gene polymorphisms and colorectal cancer risk in the Slovak population

Sentinel lymph node in colorectal cancer - 5 years follow up

Incidence of colorectal cancer in Lower Silesia (Poland) between 1984 and 2003 - trends and perspectives

Altered membrane amino acids composition in human colorectal cancer tissue

ANTITUMOR EFFECTS OF VANILLIN BASED CHALCONE ANALOGUES IN VITRO

Zastosowanie probiotyków w profilaktyce oraz leczeniu raka żołądka i raka jelita grubego