Search results

1

Karyotypes and C-banding patterns of some Phaneropterinae katydids (Orthoptera, Tettigonioidea) with special attention to a post-reductional division of the neo-X and the neo-Y sex chromosomes in Isophya hemiptera

100%

Warchalowska-Sliwa E., Bugrov A. G.

Folia Biologica

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1998

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vol. 46

47-54

EN

Karyotypes and C-banding patterns of four species belonging to three genera of the subfamily Phaneropterinae were studied. The basic karyotype of Isophya kalishevskii, Polysarcus zacharovi, and Poecilimon ukrainicus consists of 2n=31(XO) in the male. The chromosome number of Isophya hemiptera is 2n%=28+neo-X+neo-Y as a result of mutual tandem translocation between the originally acrocentric X-chromosome and acrocentric medium size autosome. Analysis of the meiotic behaviour of the neo-X and neo-Y demonstrated a post-reductional division of these chromosomes.

2

Cytogenetic markers for X chromosome in a karyotype of rainbow trout from Rutki strain (Poland)

100%

Ocalewicz K.

Folia Biologica

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2002

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vol. 50

|

issue 1-2

13-16

EN

Cytogenetic or molecular identification of sex chromosomes could help in breeding studies in producing monosex fish stocks, estimating success of androgenesis, gynogenesis, etc. Among fish species sex chromosomes are recognizable in only a few cases. Some populations of rainbow trout Oncorhynchus mykiss show morphologically differentiated sex chromosomes. A strain from Rutki, Poland, showed a heteromorphic pair of subtelocentric chromosome: presumably of the XY type in the male and XX in the female. Restriction endonuclease and DAPI banding resulted in a characteristic banding pattern enabling identification of the X chromosome.

3

Chromosome study of peled (Coregonus peled, Salmoniformes)

100%

Kirtiklis L., Jankun M.

|

EN

Chromosomes of Coregonus peled were examined by Giemsa, CMA3, Ag-NOR and C-banding. The karyotype of peled had a diploid number 2n=76, arm number NF=96 and consisted of twenty meta-submeta chromosomes and 56 subtelo-acrocentric chromosomes. C-positive blocks of heterochromatin were observed on the telomeric regions of meta- and submetacentric chromosomes. Pairs no. 1 and 11 had short arms, completely heterochromatic. TheNORwas observed at one acrocentric pair, no. 11. Arm length polymorphism was observed on the NOR-bearing pair.

4

Significance of chromosomal markers in the diagnosis of mantle cell lymphoma (MCL)

100%

Woroniecka R., Pienkowska-Grela B., Grygalewicz B., Rygier J., Witkowska A., Rymkiewicz G., Jarosinska-Romejko J.

Journal of Applied Genetics

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2002

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vol. 43

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issue 4

545-553

EN

According to the REAL/WHO classification, the diagnosis of mantle cell lymphoma (MCL) should be based on clinical, histopathological, immunological and cytogenetic or molecular data. This study is based on 13 cases, which were initially diagnosed as MCL with the use of conventional cytogenetic method and fluorescent in situ hybridization (FISH). MCL is associated with a specific cytogenetic aberration t(11;14)(q13;q32). The chromosomal analyses confirmed the MCL diagnosis in four cases. A neartetraploid cell line and two copies of t(11;14) were observed in three cases. These results correspond with a blastoid variant of MCL, accompanied by aggressive course and poor prognosis. The presence of karyotype with t(11;14) as the sole anomaly predicts an intermediate clinical outcome. Six patients had normal karyotypes, which is characteristic for the typical form of MCL, associated with a better prognosis. In this study we show that detection of chromosomal abnormalities is useful in diagnosis of MCL and has some prognostic significance.

5

Cytogenetic characterization of the zebra mussel Dreissena polymorpha (Pallas) from Miedwie Lake, Poland

100%

Boron A., Woznicki P., Skuza L., Zielinski R.

|

EN

Cytogenetic characterization of D. polymorpha was carried out using banding techniques such as C-banding, fluorochrome CMA3 and silver nitrate treatment. The diploid chromosome number of both investigated D. polymorpha forms (typical and albinotic) was the same 2n = 32 (NF = 56). The karyotype consisted of 5 pairs of metacentric, 7 pairs of submetacentric and four pairs of subtelo-acrocentric chromosomes. Ag-NORs were located in the telomeric position on the largest subtelo-acrocentric chromosome pair. C banding patterns indicate many sites of constitutive heterochromatin mainly located in the telomeric regions and interstitially in some chromosomes. CMA3-sites were observed in almost all chromosomes; apart from the Ag-NORs sites, they were located terminally on the chromosome arms and interstitially on three chromosome pairs. Sixteen chromosomes could be counted at the diakinesis stage of meiosis. No differences in banding chromosome patterns were found neither between both analyzed forms of D. polymorpha nor between males and females.

6

Cytogenetic analysis of a Mediterranean Gobiid Fish Gobius paganellus L., 1758 from Turkey

100%

Ergene G. S., Cavas T.

Folia Biologica

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2002

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vol. 50

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issue 1-2

5-7

EN

In this study, cytogenetic analysis of Gobius paganellus, a Mediterranean Gobiid fish from the southern coast of Turkey, was carried out by modified air-dried technique. Phytohemaglutinine pretreatment was performed before the conventional air-dried technique to increase the mitotic index. Chromosomes were obtained using gill tissue. As a result, it was determined that the diploid chromosome number of Gobius paganellus is 2n=44 (NF=45), comprising one large metacentric and 43 acrocentric chromosomes.

7

Karyotypic characterization and NOR analysis by different banding techniques of Rhamdia quelen from the first plateau of the Iguacu River (Brazil)

100%

Fenocchio A. S., Swarca A. C., Cestari M. M., Dias A. L.

Folia Biologica

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2003

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vol. 51

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issue 3-4

219-222

EN

A population of R. quelen from the first plateau of the Iguacu River (Parana State, Brazil) was analyzed cytogenetically. A diploid set of 58 chromosomes was constituted by 32 M, 16 SM, 6 ST and 4 A (FN=116). In one individual a 2n=59 karyotype was determined due to the presence of one additional metacentric heterochromatic chromosome. NORs, detected by AgNO3 and CMA3 staining as well as fluorescence in situ hybridization with an 18S rDNA probe, are located at a terminal position on the short arms of a ST chromosome pair. C-banding marks the NORs and other weak bands distributed on telomeric regions of some chromosomes. These results give evidence that Rhamdia constitute, in terms of karyotypic macrostructure, a conserved group and support the idea that several synonymous species may be included in this genus.

8

Infertility and marker chromosomes: Application of molecular cytogenetic techniques in a case of inv dup(15)

100%

Vulcani-Freitas T. M., Gil-da-Silva-Lopes V. L., Varella-Garcia M., Maciel-Guerra A. T.

Journal of Applied Genetics

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2006

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vol. 47

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issue 1

89-91

EN

We report on a phenotypically normal man with infertility, whose 47,XY,+mar karyotype was studied by spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) using a chromosome-15-specific probe (LSI SNRPN). By these techniques, the marker chromosome was identified as a small inv dup (15). Possible causes for male infertility in this case are discussed.

9

C-Banded karyotype of Myocastor coypus (Molina, 1782) from Turkey (Mammalia: Rodentia)

88%

Iliker A., Arslan A., Pamuko?lu N., Albayrak I.

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2009

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vol. 57

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issue 1-2

33-36

EN

The present study reports the C-band patterns of chromosomes of Myocastor coypus from Turkey. The karyotype of M. coypus is comprised of (2n) 42 chromosomes, the number of chromosomal arms (FN) was 83 and the number of autosomal arms (FNa) was 80. The X chromosome was a medium-sized metacentric and the Y chromosome was acrocentric and the smallest in the set. Two metacentric chromosomes have secondary constrictions. Most autosomes in this species were centromeric C-positive and some autosomes had telomeric C-bands. The X chromosome has centromeric heterochromatin, while the Y chromosome appeared to be entirely heterochromatic.

10

Chromosome banding studies by replication and restriction enzyme treatment in vendace (Coregonus albula) (Salmonidae, Salmoniformes)

88%

Jankun M., Ocalewicz K., Mochol M.

Folia Biologica

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2004

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vol. 52

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issue 1-2

47-51

EN

Chromosome banding studies were performed in vendace, Coregonus albula. Original data on distribution of early and late replication regions, restriction sites (AluI, DdeI, HinfI and HaeIII) on chromosomes in this coregonid fish have been used to analyse karyotype heterochromatin differentiation. Heterochromatic bands (C-positive and not digested by restriction enzymes) have been identified as late replicating regions. Extra bands produced by the applied methods have permitted the identification of several homologous pairs. The centromeres were differentially digested by the restriction enzymes. The studied population seems to be homogenic regarding karyotype characteristics.

11

Chromosome C-banding patterns in isolated population of the grasshopper Podisma pedestris (L.) (Orthoptera, Acrididae) from the Altai Mts

88%

Bugrov A. G., Warchalowska-Sliwa E.

Folia Biologica

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2002

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vol. 50

|

issue 1-2

101-102

EN

The C-banding patterns in the embryo chromosomes of the grasshopper Podisma pedestris (L.) from the Altai Mts are reported. The additional second C-heterochromatic arms in at least five pairs of autosomes and in the X-chromosome were revealed. The paracentromeric, interstitial, and telomeric C-bands were observed. The studied population of P. pedestris shows some differences in the distribution and amount of the heterochromatin in comparison with European populations.

12

Cytognetic analysis of beef cattle

88%

Rejduch B., Slota E., Switonski M.

Genetica Polonica

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1994

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vol. 35

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issue 4

323-332

EN

A karyotpe analysis of 565 pedigree beef cattle was carried out as part of cytogenetic studies conducted at the Department of Immuno- and Cytogenetics of the National Research Institute of Animal Production.Using CBG-, GTG- and RBA- binding techniques, 16 cases of the 1;29 Robertsonian translocation were diagnosed in the Charolais breed (two bulls) and hybrids from the Blonde d' Aquitane bulls (4 bulls and 10 heifers).A homozygous form of translocation was identifiedin one heifer.A testicular biopsy and meiotic chromosome analysis were carried out in a bull-carrier of the 1;29 translocation .The presence of a trivalent, characteristic of a centric fusion, was observed.The population under study also showed XX/XY leucocytic chimerism in two young bulls.

13

Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding

88%

Wang H.-Y., Liu D.-C., Yan Z.-H., Wei Y.-M., Zheng Y.-L.

Journal of Applied Genetics

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2005

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vol. 46

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issue 4

365-369

EN

Cytological and agronomic characteristics of a F2 population from Triticum aestivum L. ? T. durum Desf. hybrids were analyzed plant by plant. Means of morphologic traits in the F2 population were similar to those of the low-value parent. On average, F2 hybrids had 36.54 chromosomes per plant, indicating that each gamete lost 2.73 chromosomes at meiosis of the F1 generation. More than half of plants had 36?39 chromosomes, so male gametes with 19?21 chromosomes seemed to be superior to the others. The distribution frequency of chromosomes in this study differed from that in a previous report, where a different tetraploid wheat was used. This shows that a different breeding strategy may need to be taken when exploiting a different tetraploid wheat. According to our results, some plants with 42 chromosomes, having all the wheat A, B and D chromosomes, would appear in the F3 population, which provides a chance to obtain stable bread wheat lines from the self-pollinated progenies. Alternatively, the desirable individuals of the F2 population were backcrossed to bread wheat, which is very useful and efficient for the improvement of bread wheat by exploiting desirable genes in durum wheat.

14

Potential genotoxicity of Fusarium mycotoxins in Vicia and Pisum cytogenetic tests

88%

Packa D.

Journal of Applied Genetics

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1998

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vol. 39

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issue 2

171-192

EN

Several fusarial toxins (DAS, DON, FUS-X and MON) were assayed for their genotoxic activity with Vicia faba var. minor and Pisum sativum used as eukaryotic, whole-organism, test systems. Four concentrations: 1, 5, 10 and 20 ?g mL-1 were applied for 24 hours, and three fixing times: 24, 48 and 72 hours after the beginning of the treatment. Nuclei and chromosomes in mitotic cells were stained by the Feulgen method. Generally, fusarial toxins in plant cells produced three types of effects: prevented proliferating cells from entering mitosis, caused mitotic alterations and were cytotoxic. Mitotic alterations included C-mitoses, a higher incidence of metaphases/anaphases, excessive condensation of chromosomes, multipolar spindles, disturbed anatelophases and micronuclei. Both type and range of each effect were related to plant species, toxin concentration and duration of the recovery period. The results obtained from our plant bioassays were compared with those reported from other assay systems.

15

Identification of three different chromosomal additions by chromosome painting using fluorescence in situ hybridization (FISH) technique

88%

Bocian E., Stanczak H., Obersztyn E., Mazurczak T.

Journal of Applied Genetics

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1996

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vol. 37

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issue 2

197-204

EN

Fluorescence in situ hybridization (FISH) is a very useful method for assessing chromosome rearrangements.When neither banding pattern nor clinical symptoms are sufficient to determine the origin of additoinal chromosomal fragments, FISH with multiple chromosome-specific libraries allows to solve this diagnostic problem rapidly.Three chromosomal additions, 7q+, 13p+ and 22 q+, found in routine cytogenetic studies performed in children with phenotypic abnormalities were analysed using FISH.This technique documented the origin of extra matrial to be derived from chromosome 16[der(7)t(7;16)(q36.3;p13.110], 18[der(13)t(13;18)(p12;q12.2)] and 22[dup(22)(q11.2q13.1)], repectively.In two cases the abnormality arose de novo, while in the third case the product of translocation t(13;18) was ,matrnal by origin.It was present in 30% of mother's lymphocytes, and in 70% of them a balanced Robertsonian translocation t(13q;15q) was found.In the present cases the chromosome analysis with both traditional banding and chromosome painting techniques, allowed to establish final clinical diagnosis.

16

Cytogenetics, embryology and fertility of red clovere (Trifolium pratense L.) with short flowers and anthocyanin spots on the leaves

88%

Kazimierski T., Kazimierska E. M.

Journal of Applied Genetics

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1995

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vol. 36

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issue 4

353-362

EN

Anthocyanin spot on the leaves of red clover is inherited indepently of light spot and uniform leaf colour.The gene determining anthocyanin spot is probably localized on the other chromosomes than the gene determining light spot on the leaves.A short flower tube is also determined by a single recessive gene of a pleiotropic action.It stimultaneously causes reduction of pistil style, thickening of the endithecium layer in anthers - entails their indehiscence, pollen grain-agglutination and often deformation, no division of part of pollen grains into a vegetative and generative cells as well as germination of a single grains in pollen sacks.The changes in the structure of endothecium and pollen grains cause almost complete male and female infertility of plants with short flower tubes.

17

Tools of the trade: diagnostics and research in domestic animal cytogenetics

88%

Iannuzzi L., Berardino_Di D.

Journal of Applied Genetics

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2008

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vol. 49

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issue 4

357-366

EN

This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase chromosomes for mapping purposes. Each section includes historical information, basic principles for the given technique, its primary use in veterinary cytogenetics, and major limitations. Supplementary material (protocols and chemicals used) are available on our website. Even though these techniques mainly refer to the Bovidae, they can be easily extended and adapted to members of other taxa.

18

Acquired pericentric inversion of chromosome 9 in acute myeloid leukemia

88%

Udayakumar A. M., Pathare A. V., Dennison D., Raeburn J. A.

Journal of Applied Genetics

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2009

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vol. 50

|

issue 1

73-76

EN

Pericentric inversion of chromosome 9 involving the qh region is relatively common as a constitutional genetic aberration without any apparent phenotypic consequences. However, it has not been established as an acquired abnormality in cancer. Among the three patients reported so far in the literature with acquired inv(9), only one had acute myeloid leukemia (AML). Here we describe an unique case where both chromosomes 9 presented with an acquired pericentric inversion with breakpoints at 9p13 and 9q12 respectively, in a AML patient with aberrant CD7 and CD9 positivity. Additionally, one der(9) also showed short arm deletion at 9p21 to the centromeric region and including the p16 gene. The constitutional karyotype was normal. This is probably the first report describing an acquired inv(9) involving both chromosomes 9 in AML. The possible significance of this inversion is discussed.

19

The behaviour of a multiple sex chromosome system in Dundocoris flavilineatus (Heteroptera: Aradidae: Carventinae) that originated by autosome-sex chromosome fusion

75%

Jacobs D. H.

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vol. 51

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issue 1-2

23-32

EN

The nominate subspecies of Dundocoris flavilineatus Jacobs occurs in indigenous evergreen forests over a wide area in KwaZulu-Natal and the Eastern Cape Province of South Africa. It has a chromosome number of 2n = 28XY, which is the ancestral number for the genus. D. flavilineatus ndabeniensis, which comprises an isolated sibling population at Ndabeni forest in northern KwaZulu-Natal, possesses a multiple sex chromosome system, presumably a X1X2Y system and has a chromosome number of 2n = 27X1X2Y. The system probably originated when an autosome and the Y-chromosome of the 28XY karyotype fused. In contrast to the situation previously described in the XY1Y2 system of D. nodulicarinus the autosomal and original Y-chromosome parts of the neo-Y chromosome seem to have a reciprocal influence on each other in terms of structure and staining intensity during prophase I. The autosomal part of the neo-Y adopts a granulate, heteropycnotic, linear structure while the original Y part is less globular than usual in structure. The neo-X chromosome (= X2) behaves like, and stays isopycnotic with the autosomes. It is connected to the neo-Y by terminal association ? probably a terminal chiasma. The sex chromosome system is post-reductional and a sex chromosome trivalent is present in all metaphase II cells. The origin and behaviour of the neo-X1X2Y sex chromosome system in D. flavilineatus ndabeniensis are described, discussed, illustrated with photomicrographs and compared to the XY1Y2 system in D. nodulicarinus. Idiograms of the karyotypes of the two subspecies of D. flavilineatus are also presented.

20

B chromosomes, translocation between B and autosomes, and C-heterochromatin polymorphism of the grasshopper Podisma sapporensis Shir. (Orthoptera, Acrididae) in Hokkaido, northern Japan

63%

Warchalowska-Sliwa E., Bugrov A., Tatsuta H., Akimoto S.

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EN

Seven categories of B chromosomes found in the brachypterus grasshopper Podisma sapporensis from Hokkaido populations differ in structure, size, and C-band content. The interchange between B and one autosome from M3 and sporadically M7 was observed in most of the populations examined. Such an interaction between standard and non-standard chromosomal set provides an insight into the integration of supernumerary chromosome. In addition, C-heterochromatin polymorphism was also identified in male karyotypes in some populations. These facts indicate P. sapporensis is a highly polymorphic species from the cytogenetic point of view.

Refine search results

Karyotypes and C-banding patterns of some Phaneropterinae katydids (Orthoptera, Tettigonioidea) with special attention to a post-reductional division of the neo-X and the neo-Y sex chromosomes in Isophya hemiptera

Cytogenetic markers for X chromosome in a karyotype of rainbow trout from Rutki strain (Poland)

Chromosome study of peled (Coregonus peled, Salmoniformes)

Significance of chromosomal markers in the diagnosis of mantle cell lymphoma (MCL)

Cytogenetic characterization of the zebra mussel Dreissena polymorpha (Pallas) from Miedwie Lake, Poland

Cytogenetic analysis of a Mediterranean Gobiid Fish Gobius paganellus L., 1758 from Turkey

Karyotypic characterization and NOR analysis by different banding techniques of Rhamdia quelen from the first plateau of the Iguacu River (Brazil)

Infertility and marker chromosomes: Application of molecular cytogenetic techniques in a case of inv dup(15)

C-Banded karyotype of Myocastor coypus (Molina, 1782) from Turkey (Mammalia: Rodentia)

Chromosome banding studies by replication and restriction enzyme treatment in vendace (Coregonus albula) (Salmonidae, Salmoniformes)

Chromosome C-banding patterns in isolated population of the grasshopper Podisma pedestris (L.) (Orthoptera, Acrididae) from the Altai Mts

Cytognetic analysis of beef cattle

Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding

Potential genotoxicity of Fusarium mycotoxins in Vicia and Pisum cytogenetic tests

Identification of three different chromosomal additions by chromosome painting using fluorescence in situ hybridization (FISH) technique

Cytogenetics, embryology and fertility of red clovere (Trifolium pratense L.) with short flowers and anthocyanin spots on the leaves

Tools of the trade: diagnostics and research in domestic animal cytogenetics

Acquired pericentric inversion of chromosome 9 in acute myeloid leukemia

The behaviour of a multiple sex chromosome system in Dundocoris flavilineatus (Heteroptera: Aradidae: Carventinae) that originated by autosome-sex chromosome fusion

B chromosomes, translocation between B and autosomes, and C-heterochromatin polymorphism of the grasshopper Podisma sapporensis Shir. (Orthoptera, Acrididae) in Hokkaido, northern Japan