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1998
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vol. 27
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issue 1
69-78
EN
As a part of the study on the composition and development of microbial mats, it was attempted to develop new more effec-tive methods for the isolation and purification of cyanobacteria. The difficulty often encountered in the isolation and obtaining of axenic cultures is commonly known and well reflected by the great variety of purification methods used. Although numerous techniques have been described, most of them either failed or were not suitable for certain types of cultures. Differential gel electrophoresis and Percoll gradient centrifugation, applied by us, appear to be more effective than some other methods in reducing the number of heterotrophic bacterial 'contaminants' present in freshly isolated cultures of cyanobacteria from marine environment.
EN
The plankton material was sampled in August 1997 from the Gulf of Gdansk. The Cyanobacterium Nodularia spumigena strain ( NSG 0897 ) was isolated from the samples. The effect of salinity on the growth of Nodularia spumigena was studied in the laboratory. Salinity had a significant effect on the number of cells, optical density, concentration of chlorophyll a , dry mass and growth rate. The Nodularia spumigena strain grew well in salinities 4-16 PSU. A salinity of 8 PSU is the best for the growth of this strain.
EN
Microorganisms modify their biochemical composition in response to environmental factors, including nutrient availability, light, temperature and salinity. This research was carried out in order to establish ways of modifying the biochemical composition of marine cyanobacteria through the optimization of environmental parameters e.g. light, temperature and salinity. The effect of salinity stress in combination with variable temperature and light intensity was studied on Synechocystis sp., strain Ol 86, isolated from the North Sea. In nature, all microorganisms are exposed to different factors. Hence, we considered all the essential factors together in different groupings. Experiments were conducted at three different temperatures, two different light intensities and four different salinities. The influence of these factors on the growth and physiology of cyanobacteria from a microbial mat of the North Sea is reported.
EN
The impact of some organotin compounds (10-200 mg dm^3) on the planktonic cyanobacterium Synechocystis aquatilis was studied. The following order of toxicity of organotins tested to the cyanobacterium culture was found: DBTCl>TPTAclTBTCl? TPTCl>TMTCl. Chlorophyll a content in the culture seemed to be more susceptible to organotins than cyanobacterial growth. The inhibition of both parameters increased significantly with the increase of compound doses, time of exposure and decrease of initial culture density. After 96 h exposure, the chlorophyll a content in the cyanobacte-rium cultures of the initial density 56 mg dry wt dm^3 was reduced for 200 mg dm^3 of DBTCl, TBTCl and TPTCl by 70%, 50% and 20% respectively, while in the cultures of the lowest initial density (10 mg dry wt dm-3) by 90%, 75% and 50%, as compared to the organotin free controls.
EN
Metal sorption capacity of Chlorella kessleri and two strains of cyanobacteria (Aphanocapsa sp., Anabaena flos-aquae) was studied. It was found that among studied organisms, dead cells of C. kessleri were the most effective sorbents of Pb, Cu, Cd and Zn. They displayed the highest cation-exchange (Cd2+/H+) capacity and bound much more Cd and Zn at pH 7 than at pH 4. The optimum pH for sorption of Pb and Cu was 6. At pH 6, dead cells of C.kessleri could bind maximally about 37 mg Cd, 38 mg Zn, 21 mg Cu and 70 mg Pb per g of dry weight. Generally, algal dead cells (0.3 g dry wt dm^3) removed 50-70% of the metal ions from 0.01 mM solutions. A surplus of calcium and magnesium caused a slight decrease of Pb, Cu, Cd and Zn sorption.
EN
HP-HPLC (reversed-phase high-performance liquid chromatography) was used to analyse chlorophyll and carotenoid pigments in cyanobacteria and algae from the Baltic Sea, belonging to different taxonomic groups. The following species were used: Cyclotella meneghiniana - diatom, Oocystis submarina - green alga and Phormidium amphibium - cyanobacterium. Investigations on a favourable method of chlorophyll and carotenoid pigment separation have been carried out. This method allowed to separate the following pigments: lutein and zeaxanthin; diadinoxanthin and fucoxanthin; chlorophyll c1 and c2, into sharp peaks. It could be presumed that the method would be useful and universal in the identification of pigments in other algal and cyanobacteria species and natural phytoplankton assemblages.
EN
This report presents results of the studies conducted in 2002 of eukaryotic algae and cyanobacteria found in the River Dunajec in the area of the newly built dam reservoirs in Czorsztyn and Sromowce. The studies demonstrated that diatoms were the dominant group in both the river (at 74 %) and the dam reservoirs (at 32 %), and that among the diatoms collected, eutraphenthic [van Dam et al. 1994] species usually prevailed. The diatom group, characterized by a broad ecological scale (tolerant), predominated slightly only at Station 3 located downstream from the dam reservoirs. This variation, which was probably connected with improved water quality in this part of the river, was the result of the sedimentation of pollutants in the dam reservoirs. Equally unexpected was the mass development of Didymosphenia geminata in this area. It occupied not only spots in the riverbed of the River Dunajec, but it also spread to the riversides and even to the river arms close to the confluence of the streams flowing from the Pieniny Mountains. The quantitatively dominant species in this area was Cladophora glomerata, which is an excellent habitat for other algae, particularly diatoms. The ultrastructure of its cell and cell wall is of great significance since it reflects the environmental conditions in which it lives. The ultrastructure of the Cladophora glomerata that occurred at different stations did not differ significantly, nor did it indicate the existence of any considerable hazard resulting from environmental pollution.
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1998
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vol. 27
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issue 1
61-68
EN
The paper presents results of studies on the influence of fresh and aged for 6-12 months aqueous solutions of nitrilotriacetic acid (NTA) on the growth of phytoplankton cells. Axenic strain of unicellular algae Scenedesmus microspina (B2-76) and cyanobacteria Chroococcus minor (A-101) (Gleocapsa minor) were used in the experiments as test organisms. These or-ganisms are common in waters of the Gulf of Gdansk and are important components in the food chain. Concentration of chlorophyll a in test cultures served as a measure of the growth of phytoplankton cell studied. NTA, when added to the culture of Scenedesmus or Chroococcus in the concentration range from 1 to 50 mmol/dm3 exerted a strong growth promoting effect on the cells. NTA solutions aged for 9-12 months stimulated the growth of cyano-bacteria Chroococcus minor to a much larger extent than green algae Scenedesmus microspina. These results indicate that the biological activity of NTA, after long-term incubation as an aqueous solution, undergoes a considerable modification. The effects biological of the end products of such a modification depend on the time of adding of NTA, its initial, concentration as well as on the sensitivity of phytoplankton strains. It is concluded therefore that, the presence of NTA in estuarine and coastal waters may significantly affect the metabolism and growth of phytoplanktonic cells, especially cyanobacteria.
EN
The structure, dynamics and chlorophyll a content of lithoreophilous communities of Cyanobacteria and algae in two periodically drying sectors of the stream: in its upper (alpine ), and middle part (forest) zones were investigated. In both sectors the diversity of species was low. After snow melt, in the upper part, the stones were successively colonized by Cyanobacteria (with Chamaesiphon polonicus (Rostafinski.) Hansgirg prevailing), and green alga Klebsormidium rivulare (Kutz.) Starmach, finally obtaining stable and abundant communities. In the middle part of the stream, a spring explosion of Klebsormidium rivulare and species from the Chlorococcales group was observed. They developed temporary, since they disappeared after the June spate. Later this area was colonised mainly by Homoeothrix janthina (Bornet et Flahault) Starmach, Hydrurus foetidus (Villars) Trevisan, and diatoms, coming from a neighbouring non drying part of the stream. The chlorophyll a content in both sectors was low (2 - 55.8 mg m ?2) .
EN
The effect of the amino acids l-cysteine (CYS-SH), l-aspartic acid (ASP-OH) and l-glutamic acid (GLU-OH), and of polycarboxylic acids (PCAs) on the biological activity of co-balt in cultures of Scenedesmus armatus (Chodat) and Synechocystis aquatilis (Sauvageau) was investigated. The influence of metal ions was determined by measurements of chlorophyll a content in the cultures and rates of carbon fixation by the phytoplankton cells. The addition of cobalt (2.5*10-6 M) to the cultures caused inhibition of growth, monitored by the chlorophyll a concentration and the photosynthetic rate of both green algae and cyano-bacteria. It was found that these acids significantly increased the chlorophyll a content in both algal species when treated with cobalt. Polycarboxylates, CYS-SH and ASP-OH also increased the rate of C-14 incorporation into algal cells in both cultures, whereas GLU-OH was effective in cultures of Synechocystis only.The results indicate that organic compounds, polycarboxylates and amino acids modify the biological activity of the cobalt. The environmental consequences of these processes are considered.
EN
Cyanobacteria (blue ? green algae) are one of the largest group of Gram ? negative, photosynthetic prokaryotes, which are morphologically diverse and highly widespread in salt and sweet water and also in terrestrial habitats. They play a significant role as primary producers in many ecosystems. Some species of Cyanobacteria are capable to grow extensively and formate toxic water blooms which can be dangerous for animal and human health. Certain species of them produce a wide variety of bioactive compounds which can have potential biotechnological cosmetical and pharmaceutical applications. Cyanobacteria have recently been identified as one of the most promising group of organisms from which large number of novel, biochemically active natural compounds can be isolated.
EN
Two species of blue-green algae have been studied under laboratory conditions in several combinations of salinity (0, 8, 16, 24 PSU), light (20, 70, 120, 170 muE m^-2 s^-1) and temperature (20, 25, 30, 35 ?C). Temperature within the range of 30-35 ?C and light intensity of 170 mE m^-2 s^-1 were the optimum to achieve a maximum growth effect in the two species examined. Salinity as an environmental factor showed that these two tested species are fresh water in origin with some tolerance to increased salinity concentration. The best growth rate for Anabaena flos-aquae was stated in 0 PSU medium for all temperature and light conditions. For Nostoc sp. div. medium with 8 PSU was the optimum one.
EN
The genus Arthrospira includes filamentous cyanobacteria with multicellular cylindrical trichomes arranged in an open helix. The biomass of Arthrospira is composed of protein (50-70%), carbohydrates, lipids and minerals. It also contains carotenoids, phycocyanin and vitamins. Arthrospira species are found in tropical and semitropical regions. Arthrospira is industrially cultivated because of its high nutritional values as well as of its therapeutic properties. Commercial production of Arthrospira is carried out in open ponds and closed bioreactors. The biomass is sold by the incorrect name ?Spirulina' as a result of confusion in nomenclature regarding the genus Arthrospira.
EN
Nutritional properties of Arthrospira have been known for hundred years. It was consumed by the Aztecs and it is still an important food source for the Kanembu tribe in Chad. The biomass of Arthrospira is the source of eight essential and twelve non-essential amino acids, as well as lipids, carbohydrates, minerals, vitamins and carotenoids. It has immunomodulative, antioxidant, antiviral and anticancerogenic properties. Arthrospira is also used for heavy metal and inorganic nutrients removal from wastewater. Arthrospira PCC 8005 strain was chosen to be a part of MELISSA system (Micro Ecological Life Support System Alternative), where it will be used to produce oxygen and as food for astronauts.
EN
Different light conditions at the open and shaded sites caused by marginal vegetation affected the structure of Cyanobacteria and algae communities, but had no effect on chlorophyll a content. In the open area Hydrurus foetidus (Villars) Trevisan, Homoeothrix janthina (Bornet et Flahault) Starmach, and diatoms (mainly Achnanthes minutissima Kutz. together with A. biasolettiana Grun. and species from the Gomphonema genus ) were the most numerous. In shadow the abundance of Hydrurus foetidus drastically decreased, whereas the diatom biomass index, Achnanthes minutissima and A. biasolettiana showed a tendency to reduce their number. On the contrary, the abundance of green algae and Cocconeis placentula Ehr. var. euglypta Ehr. increased. Chlorophyll a contents of both sites obtaining the highest values in summer - autumn and the lowest in the winter - spring seasons.
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