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1

Long-term culture preantral and early antral ovarian follicles in mammals- perspectives and recent development

100%
Katska-Ksiazkiewicz L.
|
2003
|
issue 1
129-137
EN
The ovary of mammal contains thousands of oocytes enclosed in the preantral and early antral follicles. Since more than 99% of ovarian oocytes undergo atresia, it would be of great practical benefit if these follicles could be rescued by a long-term in vitro culture leading to provide an additional source of gametes and allowing for more efficient improvement of the reproductive potential of female. Until now, research on the isolated preantral follicles are the best developed in rodents. In mouse, investigations have led to follicle growth and antrum formation, ovulation in vitro and even to a few live kids after in vitro maturation and fertilization of the oocytes recovered from in vitro cultured preantral and even primordial follicles. Recently, some progress in the development of methods that allow obtaining competent oocytes and, after IVF, even blastocysts was shown in farm animals. However, only follicles with selected size can be utilized for these purposes. The successful results are encouraging for further development of methods for culture of preantral follicles in farm animals. In the article, techniques of follicle recovery, in vitro culture systems, methods for evaluation of oocyte growth, quality and competence for maturity and IVF including our impact in development of presented methods are discussed.
2

Efficiency of direct somatic embryogenesis in Arabidopsis thaliana (L.) Heynh. under various in vitro culture conditions

88%
Gaj M. D., Czubin M.
Biotechnologia
|
2004
|
issue 1
221-235
EN
In Arabidopsis thaliana, in vitro culture of immature zygotic embryos on medium supplemented with 2,4-D results in formation of somatic embryos via direct embrogenesis (DSE). The analysis of the nature of signals/stimuli involved in determination of embryogenic response in cultured explants can reveal genetic and physiological mechanisms involved in plant embryogenesis. The key factors for DSE induction in A. thaliana are the developmental stage of the explant and the presence of 2,4-D in induction medium. The study was undertaken to analyze DSE efficiency under modified tissue culture conditions. The studied factors included: pH of induction medium, temperature during embryogenesis induction, polyamines and their precursors, genotype and origin of the explants (seed-grown or in vitro-regenerated donor plants). The significant increase of the DSE efficiency was indicated on media with higher pH (7.0-8.0) and in culture of the explants obtained from plants regenerated via secondary embryogenesis. Moreover, embryogenic potential of the regenerant-derived explants was also observed on medium lacking of 2, 4?D. Spermidine and precursors of polyamines (ornithine and arginine) included in induction medium as well as any of the tested temperatures (5,28,32C) did not stimulate the DSE efficiency in comparison to standard conditions. All 16 tested ecotypes displayed the ability for the DSE under standard culture conditions. DSE efficiency varied between the studied ecotypes, however, most of the genotypes (75%) showed high (60-100%) frequency of explants producing somatic embryos.
3

Obtainng of hybrids within the family Cucubitaceae by in vitro culture of immature embryos.III.Characteristics of hybrids of Cucurbita maxima x C.ficifolia and C.maxima x C.foetidissima

88%
Plader W., Rakoczy-Trojanowska M.
Genetica Polonica
|
1994
|
vol. 35
|
issue 1-2
11-22
4

The Influence of amifostine used alone or in combination with 2-chlorodeoxyadenosine on normal and chronic myelogenous leukemia granulocyte-macrophage progenitor cells in vitro

88%
Korycka A., Robak T.
Archivum Immunologiae et Therapiae Experimentalis
|
2000
|
vol. 48
|
issue 4
293-299
EN
We evaluated the influence of amifostine used alone or in combination with 2-chlorodeoxyadenosine (2-CdA) on the colony growth of normal and chronic myeloid leukemia (CML) granulocyte-macrophage progenitor cells (CFU-GM) in semisolid culture in vitro. Amifostine at a concentration of 1 mg/ml was either added directly to the culture medium of normal and CML CFU-GM, or mononuclear cells (MNCs) were first preincubated with amifostine at the same concentration, washed in Iscove's modified Dulbecco minimum essential medium (IDMEM) and then added to the culture medium. Amifostine used alone inhibited the growth of CML CFU-GM colonies to a higher degree than those of normal CFU-GM, but the differences were not statistically significant. Amifostine preincubated with MNCs and used together with the highest concentration of 2-CdA significantly inhibited the colony growth of CML CFU-GM as compared to 2-CdA alone (p<0.05). In contrast, the colony growth inhibition of normal CFU-GM was not significantly lower compared to 2-CdA used alone. Our studies suggest that 2-CdA used together with amifostine is more toxic to leukemic CFU-GM than to their normal counterparts.
5

Regeneration of Arabidopsis thaliana (L.) Heynh. plants via direct somatic embryogenesis

75%
Gaj M. D.
Biotechnologia
|
2001
|
issue 2
90-98
EN
In Arabidopsis biotechnology plants are regenerated in vitro via shoot organogenesis induced in callus derived from different somatic tissues. An alternative way of in vitro plant regeneration via somatic embryogenesis has not been applied in Arabidopsis so far. Recently, it was found that development of Arabidopsis somatic embryos can be induced in the culture of immature zygotic embryos and that the callus phase is not nocessary for the initiation of embryogenesis. The aim of the presented research was to determinate the in vitro culture conditions enabling high efficiency of somatic embryo induction and their conversion into plants. The influence of induction medium composition including liquid or agar medium, type and concentration of auxin, carbohydrates and ammonium sources as well as duration of auxin treatment of explants on DSE efficiency were evaluated. Advantages of described regeneration system via DSE are as follows: short time needed to induce somatic embryos (10-15 days), high efficiency of the process (up to 90% explants responded), numerous embryos produced per explant (on average 17) and high percentage of embryo conversion into fertile plants (70-80%).
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