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1

A simplified AFLP method for fingerprinting of common wheat (Triticum aestivum L.) cultivars

100%
Tyrka M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 2
131-143
EN
The simplified AFLP method was developed and evaluated for identification and genetic diversity studies of wheat cultivars. Selective primers exploited in AFLP assay based on a single cutting enzyme PstI (PstIAFLP) generated total of 111 robust fragments, including 67 (60%) monomorphic and 12 (11%) cultivar-specific markers. Average similarity between 15 cultivars was 0.650, and varied from 0.293 (?Hope? vs. ?Aurora?) to 0.865 (?Norman? vs. ?Hornet?). Mean similarities within groups of winter wheat cultivars with and without 1BL/1RS chromosome were 0.713 and 0.685, respectively. A higher variation was found in the group of spring wheats: 0.677. The obtained results confirm the usefulness of the proposed modification of the AFLP technique for diversity studies and identification of common wheat cultivars.
2

Isozyme and RAPD markers for the identification of pea, field bean and lupin cultivars

86%
Wolko B., Swiecicki W. K., Kruszka K., Irzykowska L.
Journal of Applied Genetics
|
2000
|
vol. 41
|
issue 3
151-165
EN
The limited gene pool used in breeding decreases the level of genetic diversity in legume cultivars. Morphological or physiological characters are not always sufficient for quick, easy and precise cultivar description. Isozyme variability of 33 commercial Polish pea cultivars was analysed. The level of allozyme polymorphism discovered was high enough for the identification of all cultivars within two groups: white flowering peas for human consumption and colored flowering peas for fodder. The range of RAPD marker polymorphism among three lupin crops (white lupin, narrow-leafed lupin and yellow lupin) was tested. It was possible to identify each of four white lupin cultivars by means of bands generated by two primers. Seven narrow-leafed lupin cultivars were distinguished using three other primers. Testing of RAPD marker polymorphism, supplemented in some cases with observations of seed coat color genes, allowed to identify all 12 yellow lupin cultivars. Thirteen field bean cultivars were tested by isozyme variability and RAPD polymorphism observations. Among 15 enzyme systems investigated, 10 showed a high level of inter- and intracultivar polymorphism but the range of allozyme variability discovered was useless for cultivar identification. All cultivars analysed could be distinguished by a combination of RAPD markers amplified using two primers.
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