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1

Evaluation of genetic variation of selected strawberry (Fragaria x ananassa Duch.) cultivars using ISSR technique

100%
Kuras A., Korbin M., Zurawicz E.
Biotechnologia
|
2004
|
issue 2
73-78
EN
Precise evaluation of the parental forms? genetic variation is the base of rational, modern breeding. DNA patterns obtained in ISSR (inter simple sequence repeat) reactions for 24 strawberry cultivars, frequently used in the crossing programme at the Research Institute of Pomology and Floriculture, were analysed. Diversifying genotypes, polymorphic DNA products were observed in reactions with 54 out of 90 tested primers (806-896, #9 UBC). Six primers (811-814, 824, 840) generating 44 polymorphic bands with length from 270 to 2030 bp allowed to diversify all investigated cultivars. The DNA profiles characterising each of genotypes contained 10-19 polymorphic ISSR products.
2

Analysis of plum (Prunus domestica L.) DNA polymorphism using RAPD - PCR technique

88%
Lisek A., Korbin M., Zurawicz E., Rozpara E.
Biotechnologia
|
2004
|
issue 2
76-72
EN
Precise identification of plum cultivars is desired in breeding programme of the species as well as in orchard practise. The aim of the presented studies was the determination of 19 plum cultivars Prunus domestica L. diversity. RAPD-PCR technique with 77 primers (Operon Technologies) from kits OPB, OPG, OPT and OPU was used for the analysis. Fifty-five polymorphic fragments DNA (600-2700 bp) were obtained in reactions with 33 primers. The highest number of polymorphic fragments (3-5) was observed in reactions with primers OPB 07, OPB 18, OPG 09, OPG 10 and OPT 14. The reactions with OPB 07, OPB 18 and OPG 09 allowed to diversify 15 cultivars, except 'Wegierka Zwykla' and clones 'Promis', 'Tolar', 'Nectavit'.
3

Xenia effect on seed and embryo size in cotton (Gossypium hirsutum L.)

88%
Pahlavani M. H., Abolhasani K.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 4
331-335
EN
The term xenia was coined to describe the effect of foreign pollen on the development and characters of the seed. To study its importance and consequences for various seed traits in cotton (Gossypium hirsutum L.), the effect of pollen genotype on seed and embryo weight was studied with seeds from 15 F1 hybrids. Cross-fertilization changed seed weight by up to 7.0% in relation to self-fertilization. Xenia effect significantly increased embryo weight of cross-fertilized seeds, by up to 14.4% in comparison to self-fertilized seeds. Seeds of some crosses had a lower hull content than corresponding selfed seeds. On average, the xenia effect was greater for embryo weight than for seed weight. However, in some crosses there was no difference between cross- and self-fertilized seeds for seed weight, embryo weight, moisture content and hull content. Positive xenia effects for seed weight and embryo weight may help us to establish uniform stands of vigorous hybrid seedlings, especially under unfavourable conditions. Also, larger seed and embryo weight, along with lower hull content, could result in higher oil yield. Therefore, careful choosing of genotypes as parents and of cross direction in the production of hybrid seed is very important in cotton.
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