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Anther culture response in F1 hybrids of winter wheat (Triticum aestivum L.)

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Ponitka A., Slusarkiewicz-Jarzina A.
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The effect of cold pretreatment of spikes on somatic embryo induction and ather culture response of 25 F1 winter hybrids was investigated.The efficiency of androgenic embryos was the highest when spikes were incubated at 4 C degree for 6-9 days.A total of 2242 (73%) green and 885 (27%) albino plants were obtained from 9900 cultured anthers.Anther culture response in wheat wsa found to be markedly affected by the genotype of donor plants.This percentage of green plants varied from 0 to 115.7%.The great majority of anther-derived regenerants were haploids (82.35%), while the remaining plants were spontaneous diploids (13,73%) and aneuploids (3.92%).
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