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1

Consequences of transformation of embryogenic Triticale cell

100%
Zimny J., Czaplicki A., Menke-Milczarek I., Oleszczuk S., Sowa S.
Biotechnologia
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2000
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issue 2
64-72
EN
Plants carrying foreign genes have been obtained for many crops including wheat, rice, maize, barley and Triticale. The most important aspect for practical breeding is the regeneration of whole plants from a specific cell possessing the desired agronomic properties. Particle bombardment provided the necessary breakthrough for the efficient transformation of cereals. Efficient regeneration is a prerequisite for all transformation techniques. The aim of the presented work was to study the progeny of transgenic plants of the allohexaploid cereal species Triticale. By combining an efficient regeneration system with the successful particle bombardment method we were able to obtain transgenic Triticale plants. Transgene expression was sometimes unstable and generally resulted in the decline of the expression, although some lines showing stable expression were also selected. In our laboratory several generations of androgenic doublehaploid transgenic lines have been regenerated and multiplicated. The integrated transgenes were detected in Triticale lines by in situ hybridisation method. The stability of trangenes has been studied on ten generations. A regeneration system from single cell to plant combined with microprojectile bombardment appeared to be the most efficient transformation method for Triticale. Numerous chimeric genes are now available for research. Some of these genes may appear useful in the future breeding of Triticale.
2

The role of cGMP in cells of the immune system

100%
Kobialka M., Gorczyca W. A.
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vol. 55
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issue 2
195-210
EN
Cyclic GMP is a key messenger molecule in several cellular processes. It is also an important modulator of immune response. In this paper we summarize current data concerning regulatory and modulatory function of cGMP in the cells of immune system. Metabolism of the nucletide as well as its role in processes such as cell proliferation, differentation, chemotaxis and release of mediators are described. The fields of future research are indicated as well.
3

Sodium orthovanadate affects growth of some human epithelial cancer cells (A549, HTB44, DU145,)

100%
Klein A., Holko P., Ligeza J., Kordowiak A. M.
Folia Biologica
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2008
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vol. 56
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issue 3-4
115-121
EN
Within the concentration range of 1-20 FM, orthovanadate (Na3VO4) demonstrated a time and dose-dependent inhibition of autocrine growth of the human carcinoma cell lines A549 (lung), HTB44 (kidney) and DU145 (prostate), as compared to appropriate controls (without Na3VO4). The investigation was conducted by two methods: staining with N-hexa-methylpararosaniline (crystal violet=CV) or bromide3-(4,5-dimethyltio-azo-2)-2,5-diphenyl-tetrazole (MTT). In 5, 10 and 20 FM of Na3VO4 in serum-free medium, the mean values of these two tests for A549 were approximately 40%, 45%or 65%as compared to the appropriate controls. HTB44 had the greatest opportunity (statistically insignificant) at lower vanadium concentrations (up to 10 FM), whereas at 20 FM growth inhibition of these cells was approximately 50% of the controls. DU145 showed approximately 33%, 65% and 98% growth inhibition for 5, 10 and 20 FM of Na3VO4, respectively Additionally, hypothetical curves obtained by a MANOVA test based on the CV results after 72 h incubation with Na3VO4 in serum-free medium, and an example of a time-dependent effect of Na3VO4 on A549 cells, were also presented. Sodium orthovanadate was also examined for its cytotoxic capabilities, especially its ability to induce tumor cell apoptosis; the results were compared with the effect of paclitaxel. The target cells were dyed by differential staining (HOECHST33258 and propidium iodide) after 3 h and 24 h (DU145) or 3 h and 72 h (A549) of incubation with the vanadium compound. Contrary to the two cancer cell lines (viable, apoptotic or necrotic in experimental conditions), the renal HTB44 cells were insensitive up to 15 FM Na3VO4 concentrations. After 3 h incubation with Na3VO4, both lung (A549) and prostate (DU145) cancer cells showed a slight but significant reduction in the percentage of viable cells, and an increased amount of apoptotic cells. In contrast to the lung cells, DU145 prostate cells after 24 h were more sensitive to paclitaxel than to sodium orthovanadate. In the case of lung cells, the time of incubation was prolonged (to 72 h) to allow for a study of the effect of orthovanadate in greater detail. After 72 h of incubation with Na3VO4 or paclitaxel, A549 showed a similar level of viable cells (25-32% of total cultured cells); however, the percentage of apoptotic cells was higher in the case of A549 cells . ca 36% for both drugs, but the concentration of Na3VO4 was significantly greater than paclitaxel levels.
4

The influence of factors on baculovirus / insect cell system productivity

100%
Olejnik A., Grajek W.
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2003
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issue 1
158-175
EN
The baculovirus expression vector system (BEVS) is a powerful tool for the heterologous protein production. The productivity of BEVS depends on: insect cell lines and their growth parameters, medium composition, multiplicity of infection (MOI), time of infection (TOI), quality of viral inoculum and scale of cultivation. The factors influencing foreign gene expression level in BEVS ? were the subject of the presented review.
5
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Lack of migration and neurological benefits after infusion of umbilical cord blood cells in ischemic brain injury

75%
Zawadzka M., Lukasiuk K., Machaj E. K., Pojda Z., Kaminska B.
Acta Neurobiologiae Experimentalis
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2009
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vol. 69
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issue 1
46-51
EN
The finding of stem/progenitor cells in postnatal bone marrow and umbilical cord blood, opens up a possibility of using stem cells to treat neurologic diseases. There is a controversy, whether intravenously administered human umbilical cord blood cells (HUCBC) migrate to the brain, differentiate and improve recovery after ischemia. In this study, 1-3 ?10^6 cells from non-cultured (non-committed) mononuclear HUCBC fraction were intravenously infused 1, 2, 3 or 7 days after a transient middle cerebral artery occlusion (MCAo) in adult rats. We found few human cells only in the ischemic area, localized mostly around blood vessels with few positive cells in the brain parenchyma. Timing of HUCBC delivery after ischemia or injection of Cyclosporin A at the time of delivery, had no effect on the number of human cells detected in the ischemic brain. Infusion of HUCBC did not reduce infarct volume and did not improve neurologic deficits after MCAo, suggesting that HUCBC failed to migrate/survive in the ischemic brain and did not provide significant neurological benefits.
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