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1

Changes in phytoplankton pigments composition during 2000 in different southern Baltic regions

100%
Ston J., Kosakowska A.
Oceanological Studies
|
2002
|
vol. 31
|
issue 1-2
75-89
EN
The results of differences in phytoplankton pigments composition are presented in this paper. Samples from the Southern Baltic taken during three cruises on r/v ?Oceania? (14.02 - 28.02.2000, 06.05 - 16.05.2000 and 20.09 - 01.10.2000) were examined. Qualification and quantification analyses of chlorophylls and carotenoids present in samples of naturally existing phytoplankton were based on the RP-HPLC technique. Seasonal and spatial variability in pigment characteristics was observed in the analysed seasons. Presence of: chlorophyll a, chlorophyll c1+c2, chlorophyll b, alloxanthin, zeaxanthin, fucoxanthin, peridinin, neoxanthin, diadinoxanthin - was noted in every season; violaxanthin and beta-carotene - only in May and September. Lutein was detected only in May samples. The quantity of May and September pigments exceeded 2-6 times the February pigments content. Vertical differentiation in the pigments amount was also noted. Spatial variability emphasized the influence of Vistula and Odra water masses. Different markers of phytoplankton species were found in each season: in February - cryptophytes (alloxanthin), May - diatoms (fucoxanthin) and September - cyanophytes and dinophytes (containing zeaxanthin and diadinoxanthin).
2

Carotenoid content in Lota lota (L.) individuals in various biological activity periods

100%
Czeczuga B., Czeczuga-Semeniuk E.
Folia Biologica
|
1999
|
vol. 47
|
issue 3-4
67-72
EN
The authors investigated the carotenoid content in the particular parts of Lota lota in summer, autumn, and winter, i.e. when burbots exhibit the lowest and highest activity. By means of columnar and thin-layer chromatography, the following carotenoids were found to be presenct: a-carotene, b-carotene, e-carotene, b-cryptoxanthin, neothxanthin, lutein, 3?-epi- lutein, zeaxanthin, tunaxanthin, antheraxanthin, lutein epoxide, echinenone, 3?-hydroxy- echinenone, idoxanthin, canthaxanthin, a-doradexanthin, b-doradexanthin, astaxanthin, diatoxanthin, parasiloxanthin, monadoxanthin, 7,8-dihydroparasiloxanthin, mutatoxanthin and rhodoxanthin. In the Lota lota individuals examined, the content of carotenoids was found to differ in winter and summer. The total carotenoid content ranged from 0.067 (gonads of males) of to 6.095 ?g g-1 wet weight (fins of males from December).
3

Adaptative role of carotenoids and carotenoproteins in Cyclops kolensis Lilljeborg (Crustacea: Copepoda) specimens to extremely eutrophical conditions

100%
Czeczuga B., Kozlowska M., Czeczuga-Semeniuk E.
Folia Biologica
|
2000
|
vol. 48
|
issue 3-4
77-84
EN
The authors, using column, thin-layer, and ion-exchange chromatography, investigated carotenoid and carotenoprotein complex content in Cyclops kolensis specimens from an extremely eutrophic pond. The following carotenoids were found to be present: b-carotene, b-cryptoxanthin, lutein epoxide, crustaxanthin, 4?-hydroxyechinenone, canthaxanthin, and astaxanthin. Carotenoprotein complex containing astaxanthin as the prosthetic group name g-crustacyanine was purified from Cyclops kolensis individuals examined. The authors justify the adaptative role of these pigments in Cyclops kolensis specimens in extremely eutrophical conditions.
4

Simultaneous separation of chlorophylls and carotenoids by RP-HPLC in some algae and cyanobacteria from the southern Baltic

88%
Jodlowska S., Latala A.
Oceanological and Hydrobiological Studies
|
2003
|
issue 2
81-89
EN
HP-HPLC (reversed-phase high-performance liquid chromatography) was used to analyse chlorophyll and carotenoid pigments in cyanobacteria and algae from the Baltic Sea, belonging to different taxonomic groups. The following species were used: Cyclotella meneghiniana - diatom, Oocystis submarina - green alga and Phormidium amphibium - cyanobacterium. Investigations on a favourable method of chlorophyll and carotenoid pigment separation have been carried out. This method allowed to separate the following pigments: lutein and zeaxanthin; diadinoxanthin and fucoxanthin; chlorophyll c1 and c2, into sharp peaks. It could be presumed that the method would be useful and universal in the identification of pigments in other algal and cyanobacteria species and natural phytoplankton assemblages.
5

Biotechnology to combat reactive oxygen species

63%
Nawrot R., Gozdzicka-Jozefiak A.
Biotechnologia
|
2002
|
issue 2
88-101
EN
Increased generation of reactive oxygen species (ROS) lies at the background of many diseases, including cancer. Numerous experimental data show that ROS play direct and indirect role in induction and promotion of cancer. Antioxidants, such as glutathione, bilirubin, urate, carotenoids, ascorbate, tocopherols, retinol, belong to nonenzymatic protective mechanisms against ROS antioxidents. A number of plants are rich source of egzogenic antioxidants. Genetic engineering methods are used to modify food plants in the hope that it will elevate their antioxidant content. The recent successful developments are -carotene-rich ?golden rice? and tomato. Intake of these plants could contribute to health benefits worldwide.
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