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1

Chronic effects of and on the beta-adrenergic function in neuronal and glial

100%
Legutko B., Frankiewicz T., Czyrak A., Pilc A.
Polish Journal of Pharmacology
|
1993
|
vol. 45
|
issue 2
213-218
EN
A 7-day incubation of neuronal and glial cells with (2 myM) did not reduce cAMP response to or , however such an effect was observed when the cells were incubated with imipramine in the calcium channel antagonist nifedipine (1myM).
2

The effect of cAMP and some sulphur compounds upon the activity of mercaptopyruvate sulphurtransferase and rhodanese in mouse liver

86%
Wrobel M., Frendo J.
|
1992
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vol. 40
|
issue 1-2
11-14
EN
The aim of the experiments was to evaluate the effect of administration of cysteine, methionine, thiocystine, and thiosulphate upon the activity of mercaptopyruvate sulphurtransferase (MPST) and rhodanese in mouse liver. It was found that rhodanese activity increased following thiocystine and methionine administration and showed a smaller increase after cysteine and thiosulphate. The MPST activity significantly increased after cysteine and to a lesser extent after thiocystine and thiosulphate. On the other hand, methionine seemed to exert no effect upon the enzymatic activity. The results suggested that methionine metabolic cycle in mouse liver proceeded from cysteine to sulphane sulphur as thiocystine and, therefore, these three compounds increased rhodanese activity. Thiosulphate seemed rather to be involved in metabolic changes related to maintaining the stability of the physiological thiosulphate level and activated both the enzymes.
3

Reversible inhibition of movement in the amoebae Dictyostelium discoideum and its effect on chemoattractant recognition

72%
Waligorska A., Wianecka-Skocze? M., Korohoda W.
Folia Biologica
|
2008
|
vol. 56
|
issue 3-4
123-133
EN
The cell fixatives formaldehyde and KMnO4 at low concentrations reversibly inhibit the movement of D. discoideum amoebae without directly interfering with cell viability. This inhibition of cell movement is accompanied by the decreased attachment of cells to substratum.When the tenacity and attachment of immobilized cells are artificially increased by compressing cells between two glass surfaces, the amoebae begin to move even in the presence of the fixatives. Amoebae starved for 24 hours, subjected to fixatives and a mineral salt solution in which they remained motionless, maintained chemotactic res acid and only after a few hours of active locomotion became reactive to cAMP.
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