An analysis was made of the C-banded karyotype of Paederius rubrothoracicus carpathicola Scheerp. The result indicates that the chromosome number is 2n=36 and n%=17+Xyp. The examined karyotype shows a paracentromeric position of constitutive heterochromatin in all autosomes. The y chromosome is wholly euchromatic.
The karyotype of the domestic goose A. cygnoides was characterised on the basis of R and C bands. Chromosomal preparations obtained from an in vitro culture of blood lymphocytes were stained by means of the RBG and CBG banding techniques. The first nine pairs of chromosomes were analysed by the R banding technique, while fourteen pairs of chromosomes were analysed by the C banding technique. The localisation of R bands as well as the sizes and positions of constitutive heterochromatin blocks were determined. Ideograms of R and C banded patterns for the analysed chromosomes were drawn. The morphological make-up of the analysed chromosomes was assessed.
The karyotype of theMuscovy duck originating from Cairina moschata was characterised on the basis of R and C bands. Chromosomal preparations obtained from an in vitro blood lymphocyte culture were RBG- and CBG-stained. The structures of nine and fourteen pairs of chromosomes were examined based on R bands and C bands, respectively. Ideograms of banded patterns of the analysed chromosomes were drawn. The sizes of individual constitutive heterochromatin blocks wereas measured. The morphology of the analysed chromosomes was assessed.
An analysis was made of the C-banded karyotype of Strophosoma capitatum (Deg.). The results indicate that the chromosome number is 2n=22 and n%=10+Xyp. The examined karyotype shows a pericentromeric position of constitutive heterochromatin in all autosomes. The shorter arm of the X chromosome is heterochromatic while the y chromosome is wholly euchromatic. Successive stages of spermatogenesis were analysed.
The karyotype of Apteranabropsis tonkinensis, described for the first time, is characterized by 2n_= 19 (XO) and 2n_=20 (XX). Successive stage of spermatogenesis were analysed.
The karyotype of the meadow spittlebug Philaenus spumarius (L.) was studied using conventional chromosome staining, C- and AgNOR- banding, and fluorescent CMA3- and DAPI- techniques. This is the first report on differential staining of the holocentric chromosomes of Auchenorrhyncha. The karyotype of Ph. spumarius includes 2n = 22 + XX/X0. The autosomal pair 1 is large and carries a gap in every homologue. After silver staining, NORs were revealed in both this chromosome pair and a middle-sized pair, most likely 6 or 7. In spermatocyte meiosis, the majority of bivalents formed one chiasma each. The bivalent 1 showed from 1 to 4 chiasmata, the value of 1 or 2 being prevalent. Two further bivalents also showed two chiasmata in some cells. After C-banding, terminal and interstitial dot-type C-heterochromatic blocks were revealed in the chromosomes. In 4 of 11 studied males, the autosomal pair 1 was polymorphic for an extra segment attached to one of the homologues. The segment consisted of both heterochromatic and euchromatic portions. No defined signals were observed in any chromosome treated with DAPI. After CMA3- staining, bright fluorescent signals were obtained in the NOR-bearing chromosomes, suggesting GC-rich DNA bound to the NORs.
The C-banding patterns of twelve weevil species are presented. The obtained results confirm the existence of two groups of species: with a small or large amount of heterochromatin in the karyotype. The first group comprises seven species (Apionidae: Holotrichapion pisi; Curculionidae: Phyllobius urticae, Ph. pyri, Ph. maculicornis, Tanymecus palliatus, Larinodontes turbinatus, Cionus tuberculosus). In weevils with a small amount of heterochromatin, tiny grains on the nucleus in interphase are visible, afterwards in mitotic and meiotic prophase appearing as dark dots. The absence of C-bands does not indicate a lack of heterochromatin but heterochromatic regions are sometimes so small that the condensation is not visible during the cell cycle. The second group comprises five species (Otiorhynchus niger, O. morio, Polydrusus corruscus, Barypeithes chevrolati, Nedyus quadrimaculatus) which possess much larger heteropicnotic parts of chromosomes visible during all nuclear divisions. The species examined have paracentromeric C-bands on autosomes and the sex chromosome X, except for Otiorhynchus niger, which also has an intercalary bands on one pair of autososomes. All the species examined differ in the size of segments of constitutive heterochromatin. The y heterochromosome is dot-like and wholly euchromatic in all the studied species.
Karyotypes of three weevil species, viz. Brachysomus dispar (2n=22, n=10+Xyp), Brachysomus hirtus (3n=33, n=10+X) and Strophosoma faber (2n=22, n=10+Xyp) belonging to the tribes Sciaphilini and Brachyderini, were studied using the C-banding technique. The karyotype structures of the two bisexual and one parthenogenetic species are described for the first time. Most chromosomes are meta- or submetacentric. In the two species of the genus Brachysomus, the chromosomes resemble one another in having a rather small amount of heterochromatin restricted to the pericentromeric region, visible as dark stained blocks mainly during the early stages of nuclear division. Larger bands at mitotic metaphase and diakinesis occur only in Strophosoma faber. Geographic parthenogenesis in Brachysomus hirtus is briefly discussed.
C-banded karyotypes were studied in the males of seven ladybird species from the subfamily Coccinellinae, viz. Adonia variegata (Goeze), Tythaspis sedecimpunctata (L.), Coccinella septempunctata (L.), Calvia decemguttata (L.), Calvia quatuordecimguttata (L.), Propylea quatuordecimpunctata (L.), Phyllobora vigintiduopunctata (L.). All the species, with the exception of Tythaspis sedecimpunctata (L.), possess 2n=20, n male=9+Xyp. Tythaspis sedecimpunctata (L.), however, has 2n=24, n male=11+Xy. The examined karyotypes show only a paracentromeric position of constitutive heterochromatin in all autosomes and the sex chromosome X, while the y heterochromosome is dot-like and wholly euchromatic. Successive stages of spermiogenesis were analysed.
The genus Isophya represents one of the largest orthopteran genera with about 45 species occurring in Europe. All over its range the genus includes groups of sibling species, the recognition of which is hampered by their morphological similarity. In part, some species were grouped according to their morphology: the Isophya straubei-group, the I. amplipennis-group and the I. major-group. Nevertheless,many species have not been studied using newmethods. This is particularly true for 15 taxa described from the Balkans, more or less resembling I. modesta, known mostly from old, unsatisfactorily informative descriptions. Chromosomal analysis of 25 species/subspecies of the genus Isophya showed the karyotypic evolution among species. The karyotype of 18 species/subspecies was investigated for the first time. The most remarkable changes in the sex chromosomes were the ancient acrocentric X chromosome inversions in 19 species and an X chromosome/autosome mutual tandem translocation, from which the sex determination system neo-XY originated in I. hemiptera. Karyotype differentiation has been less rapid in autosomes than in sex chromosomes, in this case interspecific autosomal differentiation has involved the distribution and quantity of C-heterochromatin as well as the number of NORs. Cytogenetic analysis revealed some changes reflecting the level of genomic organization,andtheresultssuggestsomeassociationofrelatedtaxafrombiogeographicregions.
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