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Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori

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Pan Y., Xia H., Lü P., Chen K., Yao Q., Chen H., Gao L., He Y., Wang L.
Acta Biochimica Polonica
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2009
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vol. 56
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issue 4
671-677
EN
Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
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Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction

100%
Chen H., Li Y., Chen K., Yao Q., Li G., Wang L.
Acta Biochimica Polonica
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2010
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vol. 57
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issue 4
505-511
EN
Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
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SYNTHESIS AND APPLICATION OF CHITOSAN HYDROXYAPATITE: A REVIEW

58%
Vokhidova N. R., Ergashev K. H., Rashidova S. S.
Progress on Chemistry and Application of Chitin and its Derivatives
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2022
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vol. 27
5-34
EN
In this review, the research devoted to synthesising chitosan apatites, their biologically active properties, and their application in medical practice is analysed. The data are from articles published between 2001 and 2022 on the formation of calcium- and phosphorus-containing chitosan composites and the mechanism of their interaction
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