Search results

Search:
in the keywords: Biogenic amines

Sort By:

Limit search:

Development of non-aqueous single stage derivatisation method for the determination of putrescine and cadaverine using GC-MS

100%

Awan M.

Open Chemistry

2008

vol. 6

issue 2

229-236

A single step derivatisation for the determination of putrescine and cadaverine by gas chromatography using trifluoroacetylacetone (TFAA) in methanol or ethanol was studied and optimised. The derivatives were analysed by an iontrap gas chromatograph-mass spectrometer (GC-MS) operating with electron impact ionisation with selective ion storage (EI-SIS) mode. The optimised mole ratios for TFAA/putrescine and TFAA/cadaverine reactions were 5/1 and 5.8/1 respectively with a reaction time of 15 minutes at 95oC. The retention times for the derivatised putrescine and cadaverine were 11.3 and 12.2 minutes respectively using the capillary column, CP-Sil 8CB; 30 m length x 0.25 mm i.d. x 0.25 mm film. The correlation coefficients (R2) of calibration curves for putrescine and cadaverine were 0.991 and 0.990 respectively over a concentration range of 100 ng cm−3 to 1500 ng cm−3. The method developed was found to be simple (single-stage derivatisation), rapid (15 minutes derivatisation & 14 minutes GC/MS run) and accurate (putrescine and cadaverine recoveries 94.8%–97.7%). [...]

Comprehensive evaluation of biogenic amines and related drugs’ antiradical activity using reactive 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical

100%

Sârbu C., Casoni D.

Open Chemistry

2013

vol. 11

issue 5

679-688

A comprehensive study of biogenic amines and related drugs as free radical scavengers and potential antioxidants was carried out using the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH·). The results of the effect of test duration and the relative concentration on the ability of the compounds to scavenge the DPPH· were investigated and discussed. The antiradical activity of amines was compared with the activity of some reference antioxidants (phenols, phenolic acids, flavonols and other antioxidants). Some particular insights related to the mechanism of reactivity toward the DPPH· were investigated by carrying out kinetic studies in methanol for different relative concentrations. According to the obtained results, catecholamines and especially their related drugs (D-dopa, adrenalone, S-(−)-carbidopa and isoprenaline) showed a fast reactivity towards DPPH· and the highest antiradical activity which increased in a concentration-dependent manner. Moreover, catecholamines proved to be more powerful scavengers than ascorbic and caffeic acids even at low concentrations while their related drugs (D-dopa, adrenalone and S-(−)-carbidopa) showed higher antiradical activity than gallic acid for all of the investigated concentrations. [...]

Refine search results

2 Open Chemistry

1 2013

1 2008

1 Awan M.

1 Casoni D.

1 Sârbu C.

Search results

Development of non-aqueous single stage derivatisation method for the determination of putrescine and cadaverine using GC-MS

Comprehensive evaluation of biogenic amines and related drugs’ antiradical activity using reactive 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical