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1

Evaluation of genetic diversity and uniformity of head cabbage DH lines by the use of RAPD markers

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Kaminski P., Staniaszek M., Kozik E. U.
Journal of Applied Genetics
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2003
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vol. 44
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issue 2
157-163
EN
DH lines derived from cabbage cvs. Kamienna Glowa, Slawa z Enkhuizen and Langendijker, representing R1 generation, were analysed by the use of RAPD markers for their diversity and uniformity. For the evaluation of genetic diversity, eight primers yielding informative bands were used. Of the total of 83 RAPD bands scored in this study, 16.9% were polymorphic between a set of 13 DH lines. The similarity of the DH lines, estimated by Jaccard?s coefficient, was depicted in the UPGMA dendrogram. Fourteen generated informative RAPD bands allowed the identification of DH lines developed from each cultivar. The evaluation of the uniformity for six closely related DH lines was possible by the use of three primers which generate one or two polymorphic bands. The lack of differences among ten plants of the five investigated DH lines manifested their uniformity. One line showed intraline polymorphism with two RAPD primers. The occurrence of the differences at the molecular level among ten plants indicated that their parental R0 plant was probably obtained from somatic cells, not by androgenesis.
2

An evaluation of some morphological traits in doubled haploid lines and their F1 hybrids of head cabbage Kamienna Glowa in the vegetative phase

100%
Kaminski P.
Journal of Applied Genetics
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2000
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vol. 41
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issue 4
247-252
EN
The internal stump length, head mass and head shape of doubled haploid (DH) lines and their F1 hybrids of head cabbage Kamienna Glowa were compared. It was found that the range of variation in the investigated traits of DH lines was higher than that of their F1 hybrids. The head mass of the DH lines indicated some level of inbreeding depression, but their F1 hybrids showed a significant effect of heterosis. Genes responsible for flattened head shape were dominant over rounded shape genes. The longer internal stump trait was dominant over the shorter one.
3

Organ-specificity of esterase isoenzymes in Brassica seedlings

100%
Wilkins A., Ratajczak L., Czosnowski E.
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vol. 34
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issue 2
147-152
EN
in roots epicotyls and leaves of 5-, 6-, and 7-week-old Brassica seedlings were studied. No variation in isoesterase phenotypes was found between individuals within the examined cultivar. Differences in the number and intensity of isoesterase bands between the investigated organs were observed. Distinctive isoesterase patterns were evaluated for each organ. Some esterase isoenzymes are proposed as markers of particular organs: EST 1/1 and EST 1/2 for leaves, whereas EST 2/2 and EST 2/3 for roots. Further studies be aimed at using different organo-specific isoesterase forms as markers of early stages of in vitro organogenesis in Brassica callus tissue culture.
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