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1

ATP measurement as a rapid test of microbiological contamination

100%
Czaczyk K.
Biotechnologia
|
1999
|
issue 4
179-185
EN
Bioluminescent measurement of ATP using luciferin/luciferase system is a very sensitive, specific, rapid, and convenient method in microbiology. The use of this method to quantitate viable microbial cells requires the use of various validation procedures and safeguards, depending on the specific application. Most industrial applications of bioluminescent ATP measurement involve detection and quantitation of microbial contamination in a particular product, such as the rapid identification of a critical level of contamination in output material. Cloning of the firefly luciferase gene lux promises to be a much more efficient, consistent and inexpensive source of recombinat enzyme. With the realization of this potential and the marketing of automated luminometers that will undoubtedly be improved greatly in terms of throughput capacity, bioluminescent ATP measurement is becoming the method of choise for large-scale monitoring of viable microbial biomass.
2

The use of the Vibrio harveyi luminescence mutagenicity assay as a rapid test for preliminary assessment of mutagenic pollution of marine sediments

88%
Podgorska B., Pazdro K., Wegrzyn G.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 4
409-412
EN
Mutagenic pollution of the natural environment is currently one of the most serious environmental problems. It includes the pollution of marine sediments. Therefore, rapid detection of the presence of mutagens is an important issue. Recently, we have developed a novel microbiological assay for rapid assessment of mutagenicity of samples from the natural environment. This assay is based on bioluminescence of a mutant Vibrio harveyi strain, and was shown to be useful in testing samples of marine water and plant tissues. Here we demonstrate the usefulness of this assay in preliminary assessment of mutagenic pollution of marine sediments. Mutagenicity of environmental samples taken from the Baltic Sea, is documented and compared here with a commercially available standard sediment sample (IAEA 383), which contains known amounts of mutagenic compounds. The whole procedure, from obtaining a sample in the laboratory to getting final results, is very short (less than 4 h).
3

Induction of light emission by luminescent bacteria treated with UV light and chemical mutagens

75%
Czyz A., Plata K., Wegrzyn G.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
377-389
EN
Intensity of light emission by luminescent bacteria in response to UV irradiation and chemical mutagens was tested. We demonstrated that luminescence of six strains of marine bacteria (belonging to four species: Photobacterium leiognathi, P. phosphoreum, Vibrio fischeri and V. harveyi) is significantly increased by UV irradiation relatively shortly after dilution of cultures. Such a stimulation of luminescence was abolished in cells treated with chloramphenicol 15 min before UV irradiation, indicating that effective gene expression is necessary for UV-mediated induction of light emission. These results suggest that stimulation of luminescence in UV-irradiated bacterial cells may operate independently of the quorum sensing regulation. A significant induction of luminescence was also observed upon treatment of diluted cultures of all investigated strains with chemical mutagens: sodium azide (SA), 2-methoxy-6-chloro-9-(3-(2-chloroethyl)aminopropylamino)acridine ? 2HCl (ICR-191), 4-nitro-o-phenylenediamine (NPD), 4-nitroquinolone-N-oxide (NQNO), 2-aminofluorene (2-AF), and benzo[?]pyrene. These results support the proposal that genes involved in bioluminescence belong to the SOS regulon. The use of bacterial luminescence systems in assays for detection of mutagenic compounds is discussed in the light of this proposal.
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