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1

Mystery of orphan Open Reading Frames (ORFans)

100%
Mackiewicz P., Baczkowski K., Sobczynski, Cebrat S.
Biotechnologia
|
2005
|
issue 3
7-21
EN
Genomics is a new field of biology. Its fast development is caused mainly by quick progress in large-scale genome sequencing and in computer technology. In spite of a huge number of sequenced microbial genomes available in databases, their taxonomical diversity is biased and reflects the interests of researches and facility of microorganisms' isolation and culture in laboratory conditions. More than 80% of genome sequencing projects are focused on the members of Proteobacteria, Firmicutes and Actinobacteria. Environmental genome shotgun sequencing reveals that microbial diversity is much greater than we expected. Particular levels of genomic analysis, the problems and subjects of genomics are specified and described here.
2

Resistance gene analogues of Arabidopsis thaliana: recognition by structure

100%
Chelkowski J., Koczyk G.
Journal of Applied Genetics
|
2003
|
vol. 44
|
issue 3
311-321
EN
Following completion of Arabidopsis thaliana sequencing projects, multiple resistance gene analogues (RGAs) have been identified. In this work a review of the current state of knowledge available in protein databases and scientific articles is presented. Putative resistance genes were identified by using BLAST searches as well as HMM fingerprints (the latter to infer existence of characteristic domains). The representation of all five classes of putative resistance genes in Col-0 ecotype was examined, along with the statistics on RGAs present on all five chromosomes of Arabidopsis thaliana.
3

Databases for biotechnology

100%
Nawrot M., Astriab M., Twardowski T.
Biotechnologia
|
1996
|
issue 3
172-180
EN
Bioinformatics is a very important part of biotechnology.The databases give us possibility to analyse the sequence and structure of bimolecules.It is very useful for research and industrial applications of biotechnology.In this paper we show the available database on CD-ROM and in on-line service in Poland useful for biotechnologists.This article based on the experts report written for CODATA Committee,
4

Profiling of regulatory microRNA transcriptomes in various biological processes: a review

88%
Shah A. A., Meese E., Blin N.
Journal of Applied Genetics
|
2010
|
vol. 51
|
issue 4
501-507
EN
A class of small, non-coding ribonucleic acids, termed microRNA (miRNA), has recently emerged as a new key player in the cellular control of gene expression. By either blocking translation or inducing target mRNA degradation, miRNA not only participates in regular biological processes within cells and tissues but is also involved in pathological processes. Many human malignancies have been linked to specific miRNA expression patterns, raising hopes for new approaches to therapy. While such human disease-related mechanisms have been widely discussed and frequently reviewed, miRNA's general significance in animals has been less in editorial focus, despite its obvious role in basic physiological processes, e.g. neurosensory maturation, development of fertility, and hibernation. Using selected examples, this review highlights our current knowledge of miRNA's potential and its promise as a new tool for gene regulation.
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