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1

Intra-specific differentiation of Paramecium bursaria strains by molecular methods -preliminary studies

100%
Greczek-Stachura M., Tarcz S., Przybos E.
Folia Biologica
|
2010
|
vol. 58
|
issue 1-2
35-45
EN
Ten strains of Paramecium bursaria and also P. caudatum, P. multimicronucleatum, P. tetraurelia strains (as outgroups) were characterized by using Random Amplified Polymorphic DNA (RAPD), Amplified Ribosomal DNA Restriction Analysis (ARDRA) and sequencing of the non-coding ribosomal internal transcribed spacer (ITS) regions. RAPD analysis revealed that all Paramecium bursaria strains possessed characteristic band patterns; there was a correlation between the degree of differentiation of DNA revealed by RAPD-fingerprinting and the geographic origin of a particular strain. ARDRA riboprinting (using a fragment of SSU-LSU rDNA, about 3085bp) with restriction enzymes DraI, EcoRV, HhaI, HindIII,MspI, PstI distinguished groups of P. bursaria strains with characteristic band patterns originating fromdifferent sites. Comparison of the 550bp ITS1-5.8S-ITS2 fragment showed differentiation (0.9%) of the P. bursaria strains as three main groups of strains connected by site of origin in the constructed tree.
2

Phytoplankton of humo-eutrophic Lake Plotycze (Sobiborski Landscape Park, Eastern Poland)

80%
Peczula W., Zykubek A.
|
1998
|
vol. 27
|
issue 2
45-50
EN
Phytoplankton structure (abundance, taxonomical structure, species diversity) as well as physico-chemical factors (tem-perature, pH, electrolytic conductivity, oxygen concentration) were observed in a natural, shallow, small lake. The comparison of three zones within the studied lake showed distinct trophic differentiation. The biodiversity indices, which were used in this analysis, appeared to be useful in determining the lake functions.
3

Metagenomic libraries as sources of genes useful for biotechnology

80%
Deja-Sikora E., Sikora M., Golebiewski M., Tretyn A.
Biotechnologia
|
2007
|
issue 4
125-139
EN
The vast majority of microorganisms cannot be cultured under laboratory conditions. It was estimated that over 99% of microbial genetic information are inaccessible due to the inability to isolate and culture bacteria. It is also widely known that among those uncultured organisms there are such that bear the genes which are interesting from the biotechnological point of view, i.e. coding for novel enzymes (lipases, amylases, cellulases, polymerases etc.), responsible for resistance to various chemical substances (heavy metals, aromatic compounds, pesticides, antibiotics), or the genes encoding the elements of biosynthetic pathways (for instance producing novel antibiotics). Recently, the methods have been developed that allow (i) isolation and purification of environmental DNA, (ii) construction of random fragment libraries of such DNA, and (iii) effective screening of those libraries in search for interesting genes. These methods are collectively known as ?metagenomics' or ?environmental genomics'. We aim to review the metagenomic methods, which will be done in Part I of our paper, and to present the up ? to ? date achievements and future perspectives for obtaining biotechnologically important genes from environmental samples in Part II. The main attention will be paid to soil metagenomics, as this kind of environment seems to be the most promising in terms of microbial biodiversity and the spectrum of biochemical reactions performed by inhabiting bacteria. We will treat the perspectives for isolation of novel, useful genes such as those coding for biosynthesis of antibiotics, organic compounds degrading pathways, and heavy metal resistance and prospects for their biotechnological application. Assessing the microbial biodiversity through metagenomic methods will also be covered.
4

Spatial (depth dependent) and temporal distribution of fish in sandy eulittoral of the tip of the Hel Peninsula (the Gulf of Gdansk - Baltic)

61%
Sapota M. R.
Oceanological Studies
|
2001
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vol. 30
|
issue 3-4
77-89
EN
Presence of fish from 10 species was confirmed in shallow close to shore waters on the tip of the Hel Peninsula, in period from early spring to late autumn. Zone from shore to 5 meter depth was investigated. The highest number and biomass were noticed in summer on 3 meter depth. As general biodiversity taking into account number increases with increasing depth, whereas biodiversity taking into account biomass is not so depth dependent. Flounder is the absolute dominant at all depths in investigated region. Common goby and three spined stickleback are two other significant species. Comparison of data from all investigated depths shows that sampling in the most close to shore zone (1 meter depth) let well describe fish community of near shore shallow waters in investigated area.
5

Application of reproduction biotechnology methods in animal biodiversity conservation

61%
Gajda B., Smorag Z.
Biotechnologia
|
2007
|
issue 4
55-65
EN
This article presents the potential and prospects for the use of selected reproduction biotechnology methods in animal biodiversity conservation programs. The first part focuses on biotechnological methods related to male reproduction such as artificial insemination, semen cryoconservation, sexing and spermatozoa sorting. The second part discusses biotechnological methods related to female reproduction such as superovulation, in vitro production and transfer of embryos, cryoconservation of oocytes and embryos, embryo sexing, cloning and transgenesis.
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