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EN
The majority of published genetic maps are based on Kosambi distances or on Haldane distances. For a comparison of both map distance measures, their random variability is of particular interest. For the statistic ?variance', this paper presents a relationship between Kosambi distances and Haldane distances. The results suggest that Kosambi distances exhibit a smaller random variability. The theoretical results are applied to an experimental data set for molecular AFLP markers linked to the bolting gene of sugar beet (Beta vulgaris L.).
EN
Eighteen varieties of sugar beet (Beta vulgaris L.), originating from various European countries, were compared in respect of peroxidase variability level. They were cultivated in the same experimental plot. The cultivars differed in ploidy level: one variety was tetraploid, three were diploid and 14 varieties were triploid. The cathodic peroxidase system is controlled by four independent genes, of which only one is polymorphic. Consequently, the varieties were characterised by frequencies of 3 allozymes belonging to one locus. Only one variety proved to be fully monomorphic. Genetic similarities between the cultivars were illustrated by a dendrogram (UPGMA) and show different groups of varieties not related to their ploidy level.
EN
Determination of ploidy level is a comon practice in sugar-beet breeding and seed production.Lately many laboratories have replaced microscopic chromosome counting by flow cytometric estimation of the nuclear DNA content. The possibility of direct estimation of cytological composition in anisoploid populations instead of individual plants analyses was investigated.Diploid, triploid and tetraploid sugar-beet lines as well as their mixtures were tested.The results obtained show that the suggested method is fast, simple and, after a proper transformation of the computer values, could be applied in breeding and seed production of polyploid crops.Precise estimation of contamination by small admixture of seeds or plants of undesirable ploidy, however, requires individual plant rather than population analyses.
EN
for plant cell culture was improved. Diameter of the upper part of the bioreactor, was expanded from 155 to 220 mm. Function of a draughtube was changed from a suspension riser to a downcomer. Instead of original aeration tube with holes stainless steel sintered air sparger was introduced. As a result the aeration rate was reduced sixfold for the same kLa value, and the culture foaming and cell deposit on the bioreactor wall was markedly limited.
Biotechnologia
|
2004
|
issue 2
137-145
EN
The aim of the studies was to estimate the effect of cytokinins on the production of callus and shoots from different seedling explants of sugar beet. Two diploid multigerm genotypes were used in the experiment. The seedling explants (hypocotyls, cotyledons and leaves) were cultured on MS medium supplemented with cytokinins: BAP, 2iP, TDZ in concentrations 0,2; 1,0 and 2,0 mg/l. Hypocotyls, cotyledons and leaves showed different abilites to form callus and shoots. After two weeks of culture, the induction of callus was mainly observed on the explants of hypocotyls and cotyledons, whereas adventitious shoots formed on leaf explants. Frequency of callus and shoots induction was dependent on the origin of the explants, concentration and type of the cytokinins. The highest percentage of regeneration was obtained on the explants of young leaves, on medium containing TDZ (51,5%). The optimal concentration of cytokinins was 1 mg/l. Plant regeneration on seedling explants came only under direct organogenesis.
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