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1

Screening of antibiotic-producing bacteria isolated from soil gotten within gaba, Bwari FCT, Abuja

100%

Azode V. U., Anyaegbunam B. C.

World News of Natural Sciences

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2023

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vol. 51

61-69

EN

This investigation seeks to determine the antibiotic-producing potential of bacteria in inhibiting the growth of other organisms like Staphylococcus aureus and Escherichia coli. The bacterial isolates were identified using standard cultural, morphological, and biochemical characterization. These were further screened for their antibiotic-producing potential using the Mueller Hinton agar (MHA) media. The identified bacterial isolates had a mean viable count ranging between 1.38×106 / 1,380,000 cfu/ml and 1.52×106 / 1,520,000 cfu/ml. Micrococcus roseus, Brevibacterium species, Bacillus subtilis, Bacillus anthracis, and Bacillus cereus were the predominant bacterial isolates. Nonetheless, the antibiotic test revealed that Bacillus subtilis and Bacillus anthracis showed antimicrobial activity against S. aureus, whereas Bacillus anthracis, Bacillus cereus, and Bacillus subtilis showed zones of inhibition against E. coli. This study shows that Bacillus species have the potential to produce antibiotics and can be used to control microbial growth in the future.

2

Determination of some frequently used antibiotics in waste waters using solid phase extraction followed by high performance liquid chromatography with diode array and mass spectrometry detection

88%

Opriş O., Soran M.-L., Coman V., Copaciu F., Ristoiu D.

Open Chemistry

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2013

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vol. 11

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issue 8

1343-1351

EN

This study is focused on the determination of some important antibiotics from different classes in waste water samples using solid phase extraction followed by high performance liquid chromatography with two detectors, diode array and mass spectrometer in positive ionisation mode. The investigated antibiotics include three penicillins (amoxicillin, ampicillin, penicillin G), two cephalosporins (ceftazidime, ceftriaxone), and two tetracyclines (tetracycline, doxycycline). The studied antibiotics were extracted from waste water samples using hydrophilic-lipophilic balanced cartridges. The extraction of antibiotics from water matrices was tested at several pH values. The best recoveries were obtained at pH 3 and 7 respectively. Depending on the nature of antibiotic, the limits of detection and quantification were obtained in the range of 0.07–0.92 µg mL−1 and 0.21–2.77 µg mL−1 respectively. Influent and effluent waste water samples were collected from a Waste Water Treatment Plant from Romania in order to detect the studied antibiotics. The antibiotics detected in the influent waste water samples were ceftriaxone (334 µg L−1), tetracycline (146 µg L−1) and doxycycline (110 µg L−1). In effluent waste water samples no target antibiotics were detected. [...]

3

Erysipelas in breast cancer patients after the radical mastectomy

75%

Matijasevic M., Dekic N., Kolarevic D., Milosevic S., Tomasevic Z., Jurisic V.

Open Medicine

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2012

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vol. 7

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issue 2

149-153

EN

Erysipelas is a bacterial cellulitis usually associated with Streptococcal infection. It may appear as a complication following mastectomy and radiotherapy for breast cancer. The study involved 17 cases of erysipelas of the upper limbs with a median age of 62 years. Here we described the clinical, therapeutic and evolutionary aspects of erysipelas. Our results indicated that the erysipelas appeared with an average of 9 years after mastectomy and was recurrent in three patients (17.64%). It is associated with obesity in 42% and arterial hypertension in 52.9% of patients with breast cancer after radical mastectomy. Breast cancer patients in advance stages of disease at presentation (T2+ T3) are significantly more subject to erysipelas in comparison to those patients with locoregional stage of disease at presentation (T1), Mann Whitney U-test, (p<0.05). All patients had complete response to antibiotics. The recurrence occurred in 2 patients (11.76%) who underwent radiotherapy with adjuvant tamoxifen and in one patient who underwent chemotherapy and radiotherapy combined. Based on these results it is possible to suggest that patients who received radiotherapy may have an additional risk factor for developing lymphedema and erysipelas

4

Photodynamic therapy (PDT) for disinfection of oral wounds. In vitro study

75%

Matějka Z., Adámková V., Šmucler R., Svobodová J., Hubálková H.

Open Medicine

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2012

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vol. 7

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issue 1

118-123

5

Occurrence and Antibiotic Resistance Profile of Bacteria Isolated from Freshly Sliced Ready to Eat Fruits Sold Across Major Retail Outlets within Owerri Metropolis

75%

Dike K. S.

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EN

In recent years, there has been a substantial rise in the consumption of sliced ready-to-eat fruits sold across numerous retail outlets in Nigeria. This is because of their high accessibility, convenience, and, most significantly, their lower cost than whole fruits. Nevertheless, these food items have continually acted as carriers for human diseases on a global scale. This study evaluated the occurrence and antibiogram of bacteria from ready-to-eat sliced fruits sold across major retail outlets in the Owerri metropolis. One hundred and fifty samples comprising oranges, watermelons, pineapples, and paw-paws were analysed using standard microbiological techniques. Bacteria isolates were identified using cultural, biochemical, and molecular methods. Antibiotic sensitivity was performed using the Kirby-Bauer diffusion technique. Results showed that orange samples had the highest total viable count (2.50 log cfu/g), total coliform count (2.70 log cfu/g), and faecal coliform count (2.05 log cfu/g), respectively. Staphylococcus aureus predominated in the fruit samples with a percentage occurrence of 29.4%. Isolates exhibited different resistance levels, with Staphylococcus aureus and Bacillus spp showing a high resistance against Gentamycin and ofloxacin. These findings demand further investigation due to the possible health risks of coliform bacteria, faecal coliform and antibiotic resistance. Therefore, monitoring and implementing food safety standards is necessary to protect consumers.

6

Antibacterial activity of (PVP-ZrO2) nanocomposite against pathogenic bacteria

75%

Abdulrazaq R. A., Al-Ramadhan Z. A., Khalaf H. H.

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2018

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vol. 18

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issue 2

187-194

EN

The antibacterial activity of a PVP-ZrO2 nanocomposite was investigated against pathogenic bacteria S. aureus and K. pneumoniae after antibacterial sensitivity was determined and one isolate was chosen that showed more antibiotic resistance. Herein, the Co-culture technique was used to calculate percent reduction of bacteria. The results that were obtained in this method show that ZrO2 nanoparticles have inhibitory effect against pathogenic bacteria gram negative bacteria and gram positive bacteria - with reduction of growth reaching 100% to both S. aureus and K. pnumoniae at 5, 10, 15, 20 and 25% ZrO2, compared with control. The resistance patterns of S. aureus and K.pnuemonia isolates show the Moxifloxacin (MXF) is the best antibiotic for both bacteria - with sensitivity at 100%, while resistance to Ceftriaxone (CRO) is at 90% S. aureus, and at 80% K. pnumoniae. The polymer-nanocomposite was prepared by weight percentage wt. % of (PVP) being dissolved in (10) ml of distilled water, with weight percentages 5%, 10%, 15%, 20% and 25% of ZrO2 nanoparticles added.

7

A study of antibiotic prescribing: the experience of Lithuanian and Russian GPs

75%

Jaruseviciene L., Radzeviciene-Jurgute R., Lazarus J., Jurgutis A., Ovhed I., Strandberg E., Bjerrum L.

Open Medicine

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2012

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vol. 7

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issue 6

790-799

EN

Background. Globally, general practitioners (GPs) write more than 90% of all antibiotic prescriptions. This study examines the experiences of Lithuanian and Russian GPs in antibiotic prescription for upper respiratory tract infections, including their perceptions of when it is not indicated clinically or pharmacologically. Methods. 22 Lithuanian and 29 Russian GPs participated in five focus group discussions. Thematic analysis was used to analyse the data. Results. We identified four main thematic categories: patients’ faith in antibiotics as medication for upper respiratory tract infections; patient potential to influence a GP’s decision to prescribe antibiotics for upper respiratory tract infections; impediments perceived by GPs in advocating clinically grounded antibiotic prescribing with their patients, and strategies applied in physician-patient negotiation about antibiotic prescribing for upper respiratory tract infections. Conclusions. Understanding the nature of physician-patient interaction is critical to the effective pursuit of clinically grounded antibiotic use as this study undertaken in Lithuania and the Russian Federation has shown. Both physicians and patients must be targeted to ensure correct antibiotic use. Further, GPs should be supported in enhancing their communication skills about antibiotic use with their patients and encouraged to implement a shared decision-making model in their practices.

8

Personal autonomy in the management of candidal prosthetic joint infection

63%

Villamil-Cajoto I., Eynde-Collado A., Otero L., Villacian Vicedo M.

Open Medicine

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2012

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vol. 7

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issue 4

539-541

EN

The principle of patient autonomy dominates the contemporary debate in medical ethics. Twenty-five years ago, most major medical decisions were left exclusively in the hands of physicians. Such decisions were usually made with beneficent intent but without open discussion, much less the full participation of the patient. Our case involves a patient’s decision to treat an infection with Candida species, the most common fungi affecting humans in a broad spectrum of opportunistic infections, including bones and joints. Only four recent cases of delayed reimplantation arthroplasty for candidal prosthetic joint infection have been reported, and there are no reports, besides ours, of successful non-surgical resolution of candidal prosthetic joint arthritis. We report this case as a patient’s choice with an excellent outcome.

9

Extended Spectrum Beta Lactamase (ESBL) producing enteric bacteria from hospital wastewater, Ibadan, Nigeria

63%

Falodun O. I., Oladimeji O. S.

World News of Natural Sciences

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2019

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vol. 22

62-74

EN

This study was conducted to investigate the occurrence of antibiotic resistance, including β-lactamase and extended spectrum β-lactamase production among enteric bacteria isolated from hospital wastewater from selected hospital within Ibadan. Physico-chemical analysis of hospital wastewater samples was done, enteric bacteria were isolated and identified using convectional biochemical tests while the selection of potential ESBL-producing bacteria was carried out using disc diffusion method and ESBL detection using double synergy test. The turbidity of the wastewater samples ranged between 4.45-6.5 NTU and total suspended solids ranged between 3.4- 45.5 mg/L. While electrical conductivity was between114.25-214 µs/m, the biological oxygen demand was between 25.8-31.25 mg/L and chemical oxygen demand ranged between 41.25-45.38 mg/L. Of the 200 bacteria isolated 35(17.5%) produced ESBL; 14(40%) from the tertiary hospital and 21(60%) from private hospital out of which 85.7%, 80% and 65.7% showed resistance to sulphamethxazole/Trimetoprim, streptomycin and tetracycline respectively, while resistance to meropenem (8.6%) was low. Among the ESBL-producing isolates, K. pneumonia had the highest (15(42.8%) rate of occurrence. This study revealed a need for hospital wastewater to be properly treated before discharged into water bodies and the environment to forestall the indiscriminate discharge of wastewater harbouring ESBL-producing bacteria.

10

Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia

51%

Geta K., Kebede A., Chemedissa M.

World News of Natural Sciences

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2019

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vol. 24

365-371

EN

The prevalence of antimicrobial resistance among food pathogens has increased during recent decades. In this work, pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., Shigella spp., and E. coli were isolated following standard methods. The bacterial isolates were then tested for their sensitivity to common antibiotics using the disc diffusion method on Mueller-Hinton Agar. All of the pathogenic bacteria were found to be resistant to erythromycin and almost all were sensitive to penicillin.

Refine search results

Screening of antibiotic-producing bacteria isolated from soil gotten within gaba, Bwari FCT, Abuja

Determination of some frequently used antibiotics in waste waters using solid phase extraction followed by high performance liquid chromatography with diode array and mass spectrometry detection

Erysipelas in breast cancer patients after the radical mastectomy

Photodynamic therapy (PDT) for disinfection of oral wounds. In vitro study

Occurrence and Antibiotic Resistance Profile of Bacteria Isolated from Freshly Sliced Ready to Eat Fruits Sold Across Major Retail Outlets within Owerri Metropolis

Antibacterial activity of (PVP-ZrO2) nanocomposite against pathogenic bacteria

A study of antibiotic prescribing: the experience of Lithuanian and Russian GPs

Personal autonomy in the management of candidal prosthetic joint infection

Extended Spectrum Beta Lactamase (ESBL) producing enteric bacteria from hospital wastewater, Ibadan, Nigeria

Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia