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Functional characteristics and anti-oxidative potentials of alginate and fucoidan in Sargassum fluitans found in Creek Town, Calabar

100%

Obinna E. P., Paul I. A., Kingsley A. C.

vol. 55

241-257

Seaweeds are the largest and most complex marine algae, which are taxonomically classified under three groups namely: brown algae, red algae and green algae. Sargassum fluitans is a genus of brown macro-algae, which are always found in abundance, floating on the sea. In this study, fucoidan and alginate were extracted from Sargassum fluitans, and their anti-oxidant and functional qualities were determined. Fucoidan and alginate extraction was carried out using hot Mcllvains buffer at 60 ⁰C. The test parameters monitored were the anti-oxidant activity (DPPH scavenging activity, hydroxyl radical scavenging activity, metal chelating and reducing power) and functional properties (water holding capacity, water swelling capacity, bulk density and water solubility index). The fucoidan and alginate yield from Sargassum fluitans was 11.12% and 13.11% respectively. The antioxidant properties (IC50) of fucoidan were compared to ascorbic acid and gallic acid as standards. The results of the functional properties of water holding capacity, oil holding capacity, water swelling capacity, emulsifying activity and bulk density of fucoidan and alginate are (1.153±0.10 g/g and 2.65±0.43), (1.67±0.25 and 2.11±0.34), (2.73±0.12 only for fucoidan), (80% and 89%), and (10±0.0.00 ± 0.62±0.00) respectively. The IC50 of fucoidan and alginate for hydrogen peroxide scavenging, metal chelating, hydroxyl radical scavenging, reducing power, and DPPH scavenging activities were (0.056±0.00 and 0.57±0.00), (0.069±0.00 and 0.081±0.00), (0.075±0.00 and 0.058±0.00), (0.101±0.00 and 0.078±0.04), and (0.058±0.00 and 0.073±0.00) respectively. Data from this study was carried out in triplicate and expressed as Mean ± SD, A p-value < 0.05 was considered significant. Results from the anti-oxidant activities of the extracts showed the following; the hydrogen peroxide scavenging activity of alginate and fucoidan is greater than that of ascorbic acid, the metal chelating activity of gallic acid is higher than fucoidan and alginate, the DPPH scavenging activity of fucoidan is greater than that of ascorbic acid. It was evident that fucoidan and alginate could serve as functional food source due to their functional properties and could serve as potential antioxidant sources.

Effect of addition of locust bean gum on the characteristics of alginate grains

72%

Kuswandi R. S. S., Pratama R. I., Rostini I., Afrianto E.

World News of Natural Sciences

2019

vol. 26

61-71

This research aims to determine the amount of addition of locust bean gum to produce the best characteristics of alginate grains. The research was conducted in January - February 2018 at the Fisheries Product Processing Laboratory of the Faculty of Fisheries and Marine Sciences, Padjadjaran University. The method that was used in this research is the experimental method with Completely Randomized Design with four treatments and three repititions. The parameters observed in this research include viscosity, gel strength, syneresis, stability, pH, water content, and ash content. The results of the research showed that the addition of locust bean gum in the alginate gel increases viscosity, gel strength, and decreases syneresis. The addition of 30% locust bean gum produced alginate grains with the best characteristics which had a viscosity of 337 cP, gel strength (), syneresis 3.10%, pH 3.10, moisture content 93.70%, and ash content 0.4%.

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2 World News of Natural Sciences

1 2024

1 2019

1 Afrianto E.

1 Kingsley A. C.

1 Kuswandi R. S. S.

1 Obinna E. P.

1 Paul I. A.

1 Pratama R. I.

1 Rostini I.

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Functional characteristics and anti-oxidative potentials of alginate and fucoidan in Sargassum fluitans found in Creek Town, Calabar

Effect of addition of locust bean gum on the characteristics of alginate grains