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1

Trends and perspectives of lipases applications

100%
Pilarek M., Szewczyk K. W., Wrona M.
Biotechnologia
|
2002
|
issue 2
146-164
EN
Properties of microbial lipases important in practical applications are briefly described. Applications of lipases in wide branches of industry are presented. Potential fields of lipases applications are also discussed.
2

A genom ? wide range analysis of gene expression ? application in biological sciences and medicine

100%
Pawliczak R., Kowalski M. L.
Postępy Higieny i Medycyny Doświadczalnej
|
2002
|
vol. 56
|
issue 4
537-553
EN
Microarrays are one of the latest breakthroughs in experimental molecular biology, which allow monitoring of gene expression for tens of thousands of genes in parallel and are already producing huge amounts of valuable data. Microarray RNA expression on a genome-wide range is now a proven technology, although the idea of analysis of expression many genes in one sample is not new. Since the development of clone printing technology and oligonucleotide synthesis allowed to produce high density microarray. In this publication we provide the information about the technology, available detection systems and data analysis software. Comprehensive review of current or fundamental papers using microarray technology application in immunology, rheumatoid arthritis, oncology, cystis fibrosis research, primary pulmonary hypertension, psychiatry, and allergic airways inflammation is also included.
3

Cryopreservation - a tool for long-term storage of cells, tissues and organs from in vitro culture derived

70%
Mikula A., Rybczynski J. J.
Biotechnologia
|
2006
|
issue 4
145-163
EN
Cryopreservation offers the possibility for long-term storage of genetic resources with maximal genotypic stability, using a minimum of space and maintenance. At present it is actively used all over the world for storage of plant material: seeds, pollen, spores, dormant buds or apical meristems in genebanks. The development of biotechnology led to the production of a new category of germplasm for cryostorage: in vitro obtain tissues, organs, embryos, special cell lines and genetically modified plant material. The maintenance of in vitro collections remains risky regarding losing accessions due to the contamination, human error or somaclonal variation. The classical slow cooling was the first standard protocol developed for hydrated plant tissues. This method is mainly used for cryopreservation of non-organized tissues, for example: cell suspensions and calli, or apices of cold-tolerant species. For differentiated structures, new cryopreservation techniques such as vitrification and encapsulation/dehydration procedures or droplet method are efficient and reliable. These freezing techniques have been successfully, routinely applied for cryopresevation of various plant material of temperate and tropical climate species. So far, cryopreservation procedures are developed for in vitro tissues and recalcitrant seeds of about 100 and 40 species, respectively.
4

Xylan-degrading enzymes ? characterization and application in biotechnology

70%
Tokarzewska-Zadora J., Rogalski J., Szczodrak J.
Biotechnologia
|
2005
|
issue 2
163-182
EN
Xylans as compounds of plant lignocellulosics seem to be a very useful feedstock for an effective utilization in biotechnology. This is due to their abundance and renewability as well as the possibility of producing various chemical by-products, fuels, fodder and food products from this source. On the other hand, an enzymatic removal of xylans from cellulosic pulps and coffee extracts by xylanases is very important for the production of high quality paper and coffee. This review article presents new trends in the investigations on plant xylans and their enzymatic hydrolysis by xylanases of different origin. Special attention has been paid to catalytic properties of different enzymes of xylanolytic complex, to their synergistic interactions, and to the mechanisms of their regulation, as well as molecular structure of the genes encoding particular enzymes. Most promising prospects for the practical applications of xylanases are also discussed.
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