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1

Interaction of Antisense oligonucleotides with DNA and proteins studied by fluorescence polarization spectroscopy

100%
Murakami A., Nakuara M., Uemastu H., Fujimora N., Iwase R., Yamaoka T.
Biotechnologia
|
1996
|
issue 4
153-166
EN
In order to evaluate the antisense effects in biological systems, the interaction of antisense molecules with biological substances such as DNAs and human serum proteins was studied by fluorescence depolarization spectroscopy.When an antisense oligonucleotide anchoring fluorescence (F-AS) was mixed with DNAs or proteins, the anisotropy value changed, indicating that antisense oligonucleotides appreciably interact with DNAs and serum proteins, and that such interaction may play important roles on antisense gene regulation.
2

Antisense oligonucleotides against inducible nitric oxide synthase reduce No production in RAW 264.7 cells

80%
Bilecki W., Okruszek A., Kwinkowski M., Sanak M., Przewlocki R.
Biotechnologia
|
1996
|
issue 4
173-182
EN
Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form targeted to iNOS mRNA were tested in RAW 264,7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-depended manner.Application of Lipofectin agent, which itself had no significant effect, greatly increased antisense activity.Decreased levels of the target mRNA, as demonstrated by reverse transcriptase -polymerase chain reaction (RT-PCR), suggest tha RNase H mediated mechanism.
3

Anti-HIV activities and mechanisms of antisense oligonucleotides

80%
Hatta T., Inagawa T., Kuwasaki T., Kinzuka Y., Takai K., Yokoyama S., Nakashima H., Yamamoto N., Takaku H.
Biotechnologia
|
1996
|
issue 4
116-131
EN
We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides prevent cDNA synthesis by the AMV, MMLV, and HIV reverse transcriptases.WE also describe tha long-term treatment of human immunodeficiency virus-infected cells with antisense phosphorothioate oligonucleotides.
4

Stability of antisense oligonucleotides - the possibillities of their enzymatic digestion

80%
Koziolkiewicz M.
Biotechnologia
|
1994
|
issue 4
50-54
EN
Nucleotic enzymes (5- and 3-nucleases, exo and endonucleases) can degrede short unmodified oligonucleotides applied as potential antisense agents. Better stability of oligonucleotides against nucleolytic enzymes can be achieved by chemical modification of internucleotide bonds.Stability of the phosphorothioate, phosphoroditioate and methylphosphonate analogues of oligonucleotides against nuclease P1,3-exonuclease, snake venom phosphodiesterase and other nucleases is discussed
5

Antisense makes sense in protein biosynthesis regulation

80%
Twardowski T.
Biotechnologia
|
1993
|
issue 3
134-137
EN
occurs in nature with frequency error better than 10-4. Regulation of is caused by several factors. Recently the effect of on in vivo as well as in vitro has been presented. The specific recognition of substrate by ribozyme is also determined by sense-antisense hybridization reaction of ribozymes flanking sequences.
6

Antisense oligonucleotides as a tool to study gene expression in the brain

80%
Szklarczyk A., Kaczmarek L.
|
EN
Antisense oligonucleotides (asODN) have recently been used to block specific gene expression in the rodent brain.Their target include subunits of receptors for neurotransmitters, neuropeptides and transcription factors, i.e., those proteins,whose other blocker are not known.Succesful applications of the as ODN require good understanding of their pharmacokinetics, mechanisms of action and side effects in the brain.Unfortunately, very little is known in this regard.Both intraventricular and intrastructure route of administration of phosphorodiester (0-ODN) and phosphorothioate (S-ODN) ODN to the brain were effectvely employed.However doses used,even in the case of the same analog, differ up to two orders of magnitude.Since translation arrest is belived to be an effective mechanism of ODN activity in the brain, most of the authors target the ODN to the mRNA region including the translation codon, but there are most no studies of the target mRNA levels.The paper reviews the recent development in this field, offering critical evaluation of the data.
7

Antisense strategy in translational regulation of protein biosynthesis. Perspectives on plant agrobiotechology

80%
Nawrot M., Sobkiewicz A., Twardowski T.
Biotechnologia
|
1994
|
issue 4
92-101
EN
Model research with antisense strategy on plant system at translational level (26S rRNA and 5S rRNA) are presented.The perspective application of this technology for Polish agriculture is discussed.
8

Regulation of proenkephalin gene expression by transcription factors Fos and CREB: an antisense oligonucleotide approach

80%
Ziolkowska B., Przewlocka B., Bilecki W., Machelska H., Przewlocki R.
Biotechnologia
|
1996
|
issue 4
167-172
EN
The present study investigated the effects of antisense oligonucleotides (AS ODNs) against c-fos and CREB m RNA in two models of the proenkephalin (PENK) gene induction.AS ODNs to both c-fos and CREB mRNA markedly reduced induction of the PENK gene in the rat hippocampus in vivo during seizures produced by kainic acid (KA).In contrast, in an in vitro model of the PENK gene induction by noradrenaline and dexamothasone in C6 glioma cells, the AS ODN to c-fos was without effect, whereas the AS ODN to CREB reduced the increase in the PENK mRNA level.The obtained results suggest that the transcription factors Fos and CREB may mediate induction of the PENK gene in the hippocampus, while induction of this gene in C6 glioma is mediated by CREB rather than Fos.
9

Antisense oligonucleotides to PAI-1 mRNA as potential agents for therapeutic intervention in cardiovascular diseases

80%
Cierniewski C. S., Pawlowska Z., Pluskota E., Stasiak M., Kobylanska A., Misiura K., Maciaszek A., Koziolkiewicz M., Stec W. J.
Biotechnologia
|
1996
|
issue 4
141-152
EN
In this report we discuss the possibility of using antisense oligonucleotides specific to PAI-1 mRNA to reversibly decrease PAI-1 level in blood plasma and thus prolong a half-life time endogenous plasminogen activators in circulation.
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