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1

Phage therapy: a reappraisal of bacteriophages as antibiotics

100%
Inal J. M.
Archivum Immunologiae et Therapiae Experimentalis
|
2003
|
vol. 51
|
issue 4
237-244
EN
The concept of phage therapy to treat bacterial infections was born with the discovery of bacteriophage almost a century ago. After a chequered history its current renaissance is fuelled by the dangerous appearance of antibiotic-resistant bacteria on a global scale. As a mark of this renewed interest, the unanswered problems of phage therapy are now being addressed, especially for human use. Phage therapy in agricultural, food processing and fish farming industries is already successful and this review, whilst being aware of the potential drawbacks, emphasizes the need for further carefully controlled empirical data on its efficacy and safety in treating human and animal disease, especially in view of its numerous advantages over antibiotics. Finally the potential of phage therapy against bioterrorism and the emergence of second generation phage antibacterials based on phage-derived single-protein lysis systems are addressed.
2

Cell cultures and transgenic plants in biotechnology

100%
Szopa J., Kostyn K.
Biotechnologia
|
2006
|
issue 4
7-17
EN
The applications of cell cultures and transgenic plants in several aspects of modern biotechnology are reviewed. The usefulness of cell cultures in biosynthetic pathway investigation, micropropagation, development of new varieties, genetic mapping and gene functioning analysis and industrial production of bioactive compounds are pointed out. The emphasis of this review is also laid on scientific aspects of transgenic plants. They are successfully used for promoters and other gene regulatory sequences study, investigation of plant primary and secondary metabolite pathways and for improving both the productivity and quality of crops. Since there is a risk to transgenic plant technology mainly because of antibiotic resistance used for transgenes selection, several methods have been worked out for release marker gene which are also briefly reviewed.
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