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CAG repeat polymorphism in the androgen receptor (AR) gene of SBMA patients and a control group

100%

Sulek A., Hoffman-Zacharska D., Krysa W., Szirkowiec W., Fidzianska E., Zaremba J.

Journal of Applied Genetics

2005

vol. 46

issue 2

237-239

Spinobulbar muscular atrophy (SBMA) is an X-linked form of motor neuron disease characterized by progressive atrophy of the muscles, dysphagia, dysarthria and mild androgen insensitivity. SBMA is caused by CAG repeat expansion in the androgen receptor gene. CAG repeat polymorphism was analysed in a Polish control group (n = 150) and patients suspected of SBMA (n = 60). Normal and abnormal ranges of CAG repeats were established in the control group and in 21 patients whose clinical diagnosis of SBMA was molecularly confirmed. The ranges are similar to those reported for other populations.

Androgens and FSH affect androgen receptor and aromatase distribution in the porcine ovary

100%

Slomczynska M., Szoltys M., Duda M., Sikora K., Tabarowski Z.

Folia Biologica

2003

vol. 51

issue 1-2

63-68

In the present study the authors investigated whether androgens could interact with FSH to induce aromatase and androgen receptor expression in porcine granulosa cells. Dissected whole porcine follicles (small, medium, and large) were incubated for 8 hours in M199 medium supplemented with testosterone (10-7 M), FSH (100 ng/ml) or both those hormones. After incubation, the follicles were fixed and immunostained to visualise androgen receptor and aromatase. In cultures of granulosa cells isolated from small and large follicles, oestrogen secretion was measured by appropriate RIA. Incubation of follicles with testosterone and FSH increased aromatase immunoreactivity in preantral and early antral (i.e. small) follicles. The immunostaining for androgen receptor was slightly higher in medium follicles, while such hormonal stimulation had no effect on small and large follicles. Moreover, granulosa cells isolated from small follicles cultured with both testosterone and FSH produced more estradiol than control cultures (40 pg vs. 100 pg/105cells). The level was relatively close to that obtained in the culture of control granulosa cells isolated from large preovulatory follicles (105 pg/105 cells). These results indicate that testosterone acts synergistically with FSH to increase aromatase expression in the small porcine follicles.

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1 Folia Biologica

1 Journal of Applied Genetics

1 2005

1 2003

1 Duda M.

1 Fidzianska E.

1 Hoffman-Zacharska D.

1 Krysa W.

1 Sikora K.

1 Slomczynska M.

1 Sulek A.

1 Szirkowiec W.

1 Szoltys M.

1 Tabarowski Z.

1 Zaremba J.

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CAG repeat polymorphism in the androgen receptor (AR) gene of SBMA patients and a control group

Androgens and FSH affect androgen receptor and aromatase distribution in the porcine ovary