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  1. 1 Genetica Polonica

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  1. 1 1994

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  1. 1 Adamski T.

  2. 1 Surma M.

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The influence of genotypes on production of barley haploids with Hordeum bulbosum cytoplasm

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Adamski T., Surma M.
Genetica Polonica
|
1994
|
vol. 35
|
issue 4
241-248
EN
Hordeum bulbosum (2x) was crossed to H.vulgare (2x) to estimate the influence of genotypes on production of barley haploids with H.bulbosum cytoplasm.Four H.bulbosum clones were pollinated with 11 H.vulgare cultivars and doubed haplod lines.Immature embryos were cultured on the B5 medium (Gamborg et al.1968).Seed setting, embryo differentiation, haploid plant development and haploid plant efficiency were scored and computed using multivariate analysis of variance as well as cluster and canonical variable analysis.Only the influence of H.vulgare genotypes on haploid production appeared to be significant.Haploid plant efficiency was very low (average 1.45%) and depended mainly on seed setting.Obtained barley haploids and consequently, doubled haploid lines with alien cytoplasm can constitute precise material for genetic studies dealing with cytoplasmic inheritance.
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