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Quantification of airborne birch (Betula sp .)pollen grains and allergens in Krakow

100%

Madeja J., Wypasek E., Plytycz B., Sarapata K., Harmata K.

Archivum Immunologiae et Therapiae Experimentalis

2005

vol. 53

issue 2

169-174

Birch (Betula sp.) pollen grains are the main cause of seasonal allergies in northern and central Europe. The allergen particles released from the grains are often well distributed in the air. Due to their size, airborne protein particles can easily penetrate into the lower parts of the respiratory airways and may lead to symptoms of asthma. The purpose of this paper was to quantify both Betula sp. pollen grains and allergens in the air. Materials for the investigation were collected in the spring of 2003 with two Hirst-type pollen volumetric traps. Tapes from one trap served for routine birch pollen grain counts, while those from the second for the immunodetection of birch allergens. As birch pollen allergen concentration is seen as dark spots on X-ray films densitometric measurements of the spots were used to quantify birch-pollen antigen concentrations in the air. In most instances, birch pollen counts corresponded with birch pollen allergen levels. However, on several occasions outside the pollen season, only grains or only allergens were detected. Apart from sampling variability, this could be due to faulty/dead pollen grains or submicronic airborne allergen particles. Counting intact pollen grains and antibody-based detection of allergen molecules are efficient tools in controlled allergen avoidance.

Specific allergen induced motility of peripheral blood mononuclear cells and polymorphonuclear leukocytes in insect sting allergy

88%

Zak-Nejmark T., Malolepszy J., Jutel M., Nittner-Marszalska M.

Archivum Immunologiae et Therapiae Experimentalis

1992

vol. 40

issue 2

107-111

The motility of perpheral blood mononuclear cells (MNC) and polymorphoxnuclear (PMN) leukocytes form normal and bee venom allergic subjects was investigated by a modified Boyden micropore filter method. The study comprised MNC locomotion in bee venom and gradients and PMN locomotion in bee venom and fMLP gradients. We demonstrated statistically significant increase in MNC and PMN motility towards bee venom in allergic patients group. This effect disappeared after the preincubation of MNC with anti-human IgE antiboidies. We observed no such effect in PMN leukocytes. Increased MNC motility in histamine gradient was observed only in control subjects group. Similarily significant increase in PMN locomotion towards fMLP was found in both allergic and control subjects. The results here demonstrated suggest that a specific allergen might be a chemoattractant for peripheral blood MNC and PMN leukocytes from atopics and could be capable to induce non-infectious inflammatory reactions as a result of its interaction with these sensitive cells.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2005

1 1992

1 Harmata K.

1 Jutel M.

1 Madeja J.

1 Malolepszy J.

1 Nittner-Marszalska M.

1 Plytycz B.

1 Sarapata K.

1 Wypasek E.

1 Zak-Nejmark T.

Search results

Quantification of airborne birch (Betula sp .)pollen grains and allergens in Krakow

Specific allergen induced motility of peripheral blood mononuclear cells and polymorphonuclear leukocytes in insect sting allergy