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vol. 44
131-136
EN
Application of the histochemical method for testing acetylcholiesterase (AChE, EC 3.1.1.7) combined with silver impregnation of the nervous tissue showed the absence of AChE-positive nerve structures in the superficial part of the pineal gland in adult male albino rats. It was carried out at the level of the light miscroscope. If it is assumed that the test for the presence of AChE is a form of indirect conclusion concerning the cholinergic nature of given nerve structures, then in the case of the superficial innervation of the pineal gland, the outcome of the test is negative.
EN
We studied the effect of intravenous injection of the cholinesterase inhibitor galanthamine (GAL) in doses from 0.025 to 5.0 mg/kg on electrically evoked field potentials in rat visual cortex.In all the experiments the amplitude of late components of evoked potentials was significantly reduced, while early components remained unaffected.These findings indicate that cortical cells are inhibited by acetylocholine (ACh).Furthermore, combined application of a muscarinic receptor blocker (atropine) and GAL reliably suppressed the effects of galanthamine.These observations suggest that ACh-induced inhibition may be mediated by activation of GABAergic interneurones that possess muscarinc receptors.
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