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Cryopreservation as a method to facilitate preservation of life capacity of plant cells and tissues

100%
Mikula A.
Biotechnologia
|
2008
|
issue 2
41-57
EN
This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultrastructural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/ tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.
2

Molecular basics of plant response to low temperature

100%
Kmiec B., Drynda R., Woloszynska M.
Biotechnologia
|
2005
|
issue 3
184-200
EN
Low temperature, drought and high salinity are common stress conditions that lead to similar plant cell responses. Although, in this paper, we focus on cold stress, it has to be underlined that most of the response mechanisms triggered by cold are not restricted only to this abiotic factor. We describe molecular level of plant acclimation to low temperature, including up- and down regulated genes as well as signal transduction pathway. The gene mutations that affect the ability of a plant to acclimate, as well as the methods allowing to identify genes involved in response to cold stress, are presented in this review.
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