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1

An evaluation of some morphological traits in doubled haploid lines and their F1 hybrids of head cabbage Kamienna Glowa in the vegetative phase

100%
Kaminski P.
Journal of Applied Genetics
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2000
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vol. 41
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issue 4
247-252
EN
The internal stump length, head mass and head shape of doubled haploid (DH) lines and their F1 hybrids of head cabbage Kamienna Glowa were compared. It was found that the range of variation in the investigated traits of DH lines was higher than that of their F1 hybrids. The head mass of the DH lines indicated some level of inbreeding depression, but their F1 hybrids showed a significant effect of heterosis. Genes responsible for flattened head shape were dominant over rounded shape genes. The longer internal stump trait was dominant over the shorter one.
2

Marker-assisted selection of diploid and tetraploid potatoes carrying Rpi-phu1, a major gene for resistance to Phytophthora infestans

51%
Sliwka J., Jakuczun H., Kaminski P., Zimnoch-Guzowska E.
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vol. 51
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issue 2
133-140
EN
The Rpi-phu1 gene originates from an interspecific hybrid between Solanum stenotomum and S. phureja, and confers a high level of resistance to Phytophthora infestans (late blight) in potato. The Rpi-phu1 was introduced by crossing at the diploid level into the S. tuberosum gene pool and then transferred to the tetraploid level by means of 2n gametes. Tetraploid lines carrying the Rpi-phu1 were selected for further crosses. A molecular marker GP94, linked in mapping population 97?30 with the Rpi-phu1 (6.4 cM), was applied to other unselected populations (2 diploid and 1 tetraploid), and was shown to be useful in marker-assisted selection (MAS) of the resistant individuals. GP94 was applied also in commercial breeding in 2 tetraploid populations. Although the marker allele of GP94, characteristic for the Rpi-phu1 presence, was rare in the S. tuberosum gene pool, it seemed to be common in 2 other sources of resistance to late blight: S. ruiz-ceballosii and S. kurtzianum.
3

Diversity of diploid androgenic Brussels sprout plants of R0 and R1 generations

51%
Kaminski P., Dyki B., Krzyzanowska D., Gorecka K.
Journal of Applied Genetics
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2005
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vol. 46
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issue 1
25-33
EN
Androgenic Brussels sprout plants were produced by the use of anther culture from the donor cultivar 'Philemon F1'. A total of 96 plants obtained from 20 androgenic R0 genotypes assigned as diploids were evaluated both in the generative and vegetative stage, in respect of their morphological characters: mean plant height; leaf size, colour and waxiness; leaf blade shape, blistering and attitude; number of sprouts; as well as their self-incompatibility and fertility. Androgenic R0 plants derived from each of the 20 embryos were highly diversified and differed from the donor in one or more morphological traits in the vegetative stage. Evaluated populations also varied in fertility and self-incompatibility. Six androgenic genotypes that set a sufficient amount of seeds of the R1 generation and 'Philemon F1' were evaluated in the field in respect of plant height, total and marketable yield per plot, shape of stem with sprouts, shape and density of sprouts, and spacing between sprouts. Only four diploid R0 and R1 populations may have some value for further breeding, as they are characterised by good vigour, high or medium ability for sprout generation, and sufficient fertility.
4

Evaluation of genetic diversity and uniformity of head cabbage DH lines by the use of RAPD markers

51%
Kaminski P., Staniaszek M., Kozik E. U.
Journal of Applied Genetics
|
2003
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vol. 44
|
issue 2
157-163
EN
DH lines derived from cabbage cvs. Kamienna Glowa, Slawa z Enkhuizen and Langendijker, representing R1 generation, were analysed by the use of RAPD markers for their diversity and uniformity. For the evaluation of genetic diversity, eight primers yielding informative bands were used. Of the total of 83 RAPD bands scored in this study, 16.9% were polymorphic between a set of 13 DH lines. The similarity of the DH lines, estimated by Jaccard?s coefficient, was depicted in the UPGMA dendrogram. Fourteen generated informative RAPD bands allowed the identification of DH lines developed from each cultivar. The evaluation of the uniformity for six closely related DH lines was possible by the use of three primers which generate one or two polymorphic bands. The lack of differences among ten plants of the five investigated DH lines manifested their uniformity. One line showed intraline polymorphism with two RAPD primers. The occurrence of the differences at the molecular level among ten plants indicated that their parental R0 plant was probably obtained from somatic cells, not by androgenesis.
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