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Determination of the Protein-Binding Properties of Camptothecins by Means of Optical Spectroscopy Methods

100%
Ziomkowska B., Cyrankiewicz M., Wybranowski T., Kruszewski S.
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vol. 125
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issue 4A
A-61-A-65
EN
Optical spectroscopy methods are widely used in studies of drugs. The affinity of camptothecins - anticancer agents - to human serum albumin (HSA) was determined in this work. Camptothecins (CPTs) exist in two forms: active lactone and open ring inactive carboxylate. In blood, the hydrolysis process of lactone form occurs which leads to deactivation of CPTs. Research is being done on biophysical properties of synthesized CPT compounds, in particular on binding to albumin. The affinity to plasma proteins is an important determinant of stability of CPTs in blood. The following analogues of CPT were tested in this paper: irinotecan, SN-38, topotecan, and 9-amino camptothecin. Using the method of fluorescence anisotropy measurement, the association constants of the studied compounds to HSA were determined. The authors attempted to determine the deactivation rate of topotecan in HSA solution using Principal Component Analysis and Factor Analysis of absorption spectra recorded during hydrolysis process of lactone form.
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Enhancement of FITC Fluorescence by Silver Colloids and Silver Island Films

88%
Kruszewski S., Wybranowski T., Cyrankiewicz M., Ziomkowska B., Pawlaczyk A.
Acta Physica Polonica A
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2008
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vol. 113
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issue 6
1599-1608
EN
Silver colloids and silver island films as fluorescence enhancement systems are the subject of this paper. Enhancement of fluorescence of molecules placed near metallic nanoparticles is a result of resonance interaction of exciting and emitted lights with surface plasmons. Two models explaining this phenomenon are presented. Processes of silver colloids and silver island films forming are described. Results of extinction, absorption and scattering studies of fluorescence enhancement systems are presented. The influence of these systems, i.e. silver colloids and silver island films on the fluorescence of fluorescein-5-isothiocyanate was studied. 7-fold increase in fluorescence intensity of fluorescein-5-isothiocyanate molecules placed near silver nanoparticles was observed.
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