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Structures and serology of the O-antigens of Proteus strains classified into serogroup O17 and former serogroup O35

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Torzewska A., Grabowski S., Kondakova A. N., Toukach F. V., Senchenkova S. N., Shashkov A. S., Arbatsky N. P., Knirel Y. A., Rozalski A., Kaca W.

Archivum Immunologiae et Therapiae Experimentalis

2006

vol. 54

issue 4

277-282

Introduction: Bacteria of the genus Proteus are facultative pathogens which commonly cause urinary tract infections. Based on the serological specificity of the O-chain polysaccharide of the lipopolysaccharide (O-polysaccharide, O-antigen), strains of P. mirabilis and P. vulgaris have been classified into 60 serogroups. Studies on the chemical structure and serological specificity of the O-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteria. Materials and Methods: The O-polysaccharide was prepared by acetic acid degradation of the lipopolysaccharide isolated from dried bacterial mass of each strain by hot phenol/water extraction. 1H- and 13C-NMR spectroscopy was used for structural studies. Serological studies were performed with rabbit O-antisera using enzyme immunosorbent assay, passive hemolysis test, and the inhibition of reactions in these assays as well DOC-PAGE and Western blot. Results: Four Proteus strains belonging to serogroups O17 and O35 were found to possess similar O-polysaccharide structures, in particular having the same carbohydrate backbone built up of tetrasaccharide repeating units. However, they differ in the presence or absence of additional substituents, such as phosphoethanolamine in P. mirabilis O17 and glucose in P. penneri O17, as well as in the pattern and degree of O-acetylation of various monosaccharide residues. Serological studies also showed close relationships between the O-antigens studied. Conclusions: Based on these data it is proposed to reclassify strain P. mirabilis PrK 61/57, formerly representing the O35 serogroup, into the serogroup O17 in the Kauffman-Perch classification system of Proteus.

Structural and serological characterization of the lipopolysaccharide from proteus penneri 20 and classification of cross-reacting proteus penneri strains 10, 16, 18, 20, 32 and 45 in proteus serogroup O17

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Sidorczyk Z., Toukach F. V., Zych K., Arbatsky N. P., Drzewiecka D., Ziolkowski A., Shashkov A. S., Knirel Y. A.

Archivum Immunologiae et Therapiae Experimentalis

2002

vol. 50

issue 5

345-355

O-specific polysaccharide (O-antigen) of the lipopolysaccharide of Proteus penneri 20 was studied using sugar analysis along with various one- and two-dimensional NMR spectroscopy techniques. The structure of the polysaccharide was established. It has the same carbohydrate backbone structure as that described earlier for P. penneri 16, in which the positions of the O-acetyl groups have not been determined. P. penneri 20 O-antiserum showed a strong cross-reactivity with the lipopolysaccharides of P. penneri 10, 16, 18, 32, 45 and P. mirabilis O17. These data enable classifying these strains together with P. penneri 20 in one Proteus serogroup, O17.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2006

1 2002

2 Arbatsky N. P.

2 Knirel Y. A.

2 Shashkov A. S.

2 Toukach F. V.

1 Drzewiecka D.

1 Grabowski S.

1 Kaca W.

1 Kondakova A. N.

1 Rozalski A.

1 Senchenkova S. N.

1 Sidorczyk Z.

1 Torzewska A.

1 Ziolkowski A.

1 Zych K.

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Structures and serology of the O-antigens of Proteus strains classified into serogroup O17 and former serogroup O35

Structural and serological characterization of the lipopolysaccharide from proteus penneri 20 and classification of cross-reacting proteus penneri strains 10, 16, 18, 20, 32 and 45 in proteus serogroup O17