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Androgens and FSH affect androgen receptor and aromatase distribution in the porcine ovary

100%

Slomczynska M., Szoltys M., Duda M., Sikora K., Tabarowski Z.

Folia Biologica

2003

vol. 51

issue 1-2

63-68

In the present study the authors investigated whether androgens could interact with FSH to induce aromatase and androgen receptor expression in porcine granulosa cells. Dissected whole porcine follicles (small, medium, and large) were incubated for 8 hours in M199 medium supplemented with testosterone (10-7 M), FSH (100 ng/ml) or both those hormones. After incubation, the follicles were fixed and immunostained to visualise androgen receptor and aromatase. In cultures of granulosa cells isolated from small and large follicles, oestrogen secretion was measured by appropriate RIA. Incubation of follicles with testosterone and FSH increased aromatase immunoreactivity in preantral and early antral (i.e. small) follicles. The immunostaining for androgen receptor was slightly higher in medium follicles, while such hormonal stimulation had no effect on small and large follicles. Moreover, granulosa cells isolated from small follicles cultured with both testosterone and FSH produced more estradiol than control cultures (40 pg vs. 100 pg/105cells). The level was relatively close to that obtained in the culture of control granulosa cells isolated from large preovulatory follicles (105 pg/105 cells). These results indicate that testosterone acts synergistically with FSH to increase aromatase expression in the small porcine follicles.

Ectopic bone formation after treatment with colchicine

73%

Tworek K., Solak K., Janeczek A., Niedzwiedzki T., Tabarowski Z., Dabrowski Z., Schoutens A., Tworek S.

Folia Biologica

2010

vol. 58

issue 1-2

125-133

We followed changes occurring within bone tissue and marrow cells during the process of colchicine-induced ectopic bone development and its resorption inside the marrow cavity of the rat tibia. To stimulate ectopic bone formation male Wistar rats were i.p injected with 0.5 or 1mg/kg b.w. of colchicine orwith a 100 Fg intra-bone injection.Not all subjects responded to colchicine with ectopic bone formation in the marrow cavity, even among individuals belonging to the same strain. The kind of response in a given animal depended on the dose and site of colchicine administration. During 10 days of the experiment an increase in the occurrence of micronuclei in the polychromatic erythrocytes residing in the bone marrow (even 40-fold) was observed, indicating high genotoxicity of colchicine (at a dose of 1 mg/kg b.w. i.p. or 100 Fg intra-bone injection). An increase in the frequency of emperipolesis in megakaryocytes between the 4th and 8th days of the experiment was caused by the toxic action of colchicine and may indicate the labilisation of cell membranes and microtubule depolymerisation.

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2 Folia Biologica

1 2010

1 2003

2 Tabarowski Z.

1 Dabrowski Z.

1 Duda M.

1 Janeczek A.

1 Niedzwiedzki T.

1 Schoutens A.

1 Sikora K.

1 Slomczynska M.

1 Solak K.

1 Szoltys M.

1 Tworek K.

1 Tworek S.

Search results

Androgens and FSH affect androgen receptor and aromatase distribution in the porcine ovary

Ectopic bone formation after treatment with colchicine