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1

Analiza uszkodzeń DNA w komórkach ssaków metodą elucji alkalicznej

100%

Szmigiero L., Łodzi A. M. w.

Acta Universitatis Lodziensis. Folia Biochimica et Biophysica

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1999

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vol. 14

EN

DNA alkaline elution is one of the most prominent methods which are currently in use for investigation of the effects of genotoxic agents. The main advantage of this technique is its high sensitivity as well as versatility that allows one to m easure a variety of DNA lesions. Basic principles of the method and its research applications are reviewed.

2

DNA-protein cross-linking induced by nitracrine in two strains of mouse lymphoma L5178Y cells

51%

Ciesielska E., Walicka M., Pastwa E., Szmigiero L.

Acta Biochimica Polonica

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1992

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vol. 39

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issue 1

53-57

3

Structure of acridines and their effect on RNA synthesis in vitro

51%

Szmigiero L., Jaros-Kamińska K. Ś. E. C. B., Gniazdowski M.

Acta Biochimica Polonica

|

1977

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vol. 24

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issue 1

35-44

4

Inhibition of mammalian topoisomerase I by 1-nitro- 9-aminoacridines. Dependence on thiol activation

51%

Ciesielska E., Pastwa E., Szmigiero L.

Acta Biochimica Polonica

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1997

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vol. 44

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issue 4

775-780

5

Sister chromatid exchanges induced in vitro in human lymphocytes by N-substituted phosphorodiamidic acids

45%

Ciesielska E., Mordalska A., Dzwonkowska A., Kinas R., Szmigiero L.

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vol. 40

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issue 1

77-79

6

Cytotoxicity and inhibitory properties against topoisomerase II of doxorubicin and its formamidine derivatives

45%

Kik K., Studzian K., Wąsowska-Łukawska M., Oszczapowicz I., Szmigiero L.

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issue 1

135-142

EN

This work was undertaken to compare cytotoxicity, DNA damaging properties and effect on DNA cleavage by topoisomerase II of the anthracycline drug doxorubicin (DOX) and its two derivatives with a formamidino group containing a cyclic amine moiety such as morpholine (DOXM) or hexamethyleneimine (DOXH). The tetrazolium dye colorimetric assay was used to determine the cytotoxic activity of anthracyclines toward L1210 leukemia cells. DNA damage was measured by alkaline elution technique. The effect of anthracyclines on DNA cleavage was studied in a cell-free system containing supercoiled pBR322 DNA and purified human topoisomerase II. The cytotoxicity data and the results of studies on the mechanism of DNA break formation by anthracyclines at the cellular level and in the cell-free system showed that the presence of the formamidino group in the doxorubicin molecule reduced its ability to stimulate DNA cleavage by DNA topoisomerase II. Conclusion: DNA topoisomerase II is not a primary cellular target for DOXM or DOXH. An advantageous feature of formamidinoanthracyclines is their mechanism of cytotoxic action which is not related to the inhibition of DNA topoisomerase II. Therefore this class of anthracyclines seems to be a good source for selection of an anticancer drug directed toward cancer cells with the developed multidrug resistance attributed to the presence of altered DNA topoisomerase II.

7

DNA-protein crosslinks induced in HeLa cells by bis-1-nitroacridines

45%

Pastwa E., Ciesielska E., Studzian K., Szmigiero L.

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vol. 40

|

issue 1

69-71

8

Thiol dependent inhibition of mouse leukemia L1210 DNA topoisomerase I by nitracrine

45%

Ciesielska E., Szmigiero L.

Acta Biochimica Polonica

|

1994

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vol. 41

|

issue 2

141-143

9

Uptake of acridinecarboxamide derivatives by L1210 cells

39%

Ciesielska E., Studzian K., Bazylak G., Pastwa E., Denny W. A., Szmigiero L.

Acta Biochimica Polonica

|

1998

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vol. 45

|

issue 4

1047-1051

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8 Acta Biochimica Polonica

1 Acta Universitatis Lodziensis. Folia Biochimica et Biophysica

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1 1992

1 1977

9 Szmigiero L.

6 Ciesielska E.

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3 Studzian K.

1 Bazylak G.

1 Denny W. A.

1 Dzwonkowska A.

1 Gniazdowski M.

1 Jaros-Kamińska K. Ś. E. C. B.

1 Kik K.

1 Kinas R.

1 Mordalska A.

1 Oszczapowicz I.

1 Walicka M.

1 Wąsowska-Łukawska M.

1 Łodzi A. M. w.

Analiza uszkodzeń DNA w komórkach ssaków metodą elucji alkalicznej

DNA-protein cross-linking induced by nitracrine in two strains of mouse lymphoma L5178Y cells

Structure of acridines and their effect on RNA synthesis in vitro

Inhibition of mammalian topoisomerase I by 1-nitro- 9-aminoacridines. Dependence on thiol activation

Sister chromatid exchanges induced in vitro in human lymphocytes by N-substituted phosphorodiamidic acids

Cytotoxicity and inhibitory properties against topoisomerase II of doxorubicin and its formamidine derivatives

DNA-protein crosslinks induced in HeLa cells by bis-1-nitroacridines

Thiol dependent inhibition of mouse leukemia L1210 DNA topoisomerase I by nitracrine

Uptake of acridinecarboxamide derivatives by L1210 cells