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1

Understanding evolution

100%

Barciszewski J., Legocki A. B.

|

2001

|

vol. 48

|

issue 2

291-294

2

Reduction of bacterial genome size and expansion resulting from obligate intracellular lifestyle and adaptation to soil habitat.

100%

Stępkowski T., Legocki A. B.

|

2001

|

vol. 48

|

issue 2

367-381

EN

Prokaryotic organisms are exposed in the course of evolution to various impacts, resulting often in drastic changes of their genome size. Depending on circumstances, the same lineage may diverge into species having substantially reduced genomes, or such whose genomes have undergone considerable enlargement. Genome reduction is a consequence of obligate intracellular lifestyle rendering numerous genes expendable. Another consequence of intracellular lifestyle is reduction of effective population size and limited possibility of gene acquirement via lateral transfer. This causes a state of relaxed selection resulting in accumulation of mildly deleterious mutations that can not be corrected by recombination with the wild type copy. Thus, gene loss is usually irreversible. Additionally, constant environment of the eukaryotic cell renders that some bacterial genes involved in DNA repair are expandable. The loss of these genes is a probable cause of mutational bias resulting in a high A+T content. While causes of genome reduction are rather indisputable, those resulting in genome expansion seem to be less obvious. Presumably, the genome enlargement is an indirect consequence of adaptation to changing environmental conditions and requires the acquisition and integration of numerous genes. It seems that the need for a great number of capabilities is common among soil bacteria irrespective of their phylogenetic relationship. However, this would not be possible if soil bacteria lacked indigenous abilities to exchange and accumulate genetic information. The latter are considerably facilitated when housekeeping genes are physically separated from adaptive loci which are useful only in certain circumstances.

3

Glutamine synthetase in Lupinus luteus. Identification and preliminary characterization of nodule-specific cDNA clone

81%

Boroń L., Szczygłowski K., Konieczny A., Legocki A. B.

Acta Biochimica Polonica

|

1989

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vol. 36

|

issue 3-4

295-301

4

Peptidyltransferase activity of 60S ribosomal subunit from wheat germ

81%

Sikorski M. M., Kukhanova M. K., Krayevsky A., Legocki A. B.

Acta Biochimica Polonica

|

1982

|

vol. 29

|

issue 1-2

57-63

5

Cloning and identification of lupin nodule specific cDNA sequences

81%

Konieczny A., Szczygłowski K., Mądrzak C. J., Legocki A. B.

|

issue 1

21-27

6

Isolation and classification of a family of cyclin gene homologues in Lupinus luteus

81%

Deckert J., Jeleńska J., Zaborowska Ż., Legocki A. B.

Acta Biochimica Polonica

|

1997

|

vol. 44

|

issue 1

37-42

7

Characterization and expression analysis of the yellow lupin (Lupinus luteus L.) gene coding for nodule specific proline-rich protein.

81%

Karłowski W. M., Stróżycki P. M., Legocki A. B.

|

2000

|

vol. 47

|

issue 2

371-383

EN

The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.

8

Reverse transcription and kinetic complexity analysis of mRNA from lupin root nodules

80%

Mądrzak C. J., Konieczny A., Legocki A. B.

Acta Biochimica Polonica

|

1984

|

vol. 31

|

issue 4

439-451

9

Identification by affinity chromatography of wheat germ ribosomal proteins which interact with RNA

80%

Przybył D., Sikorski M. M., Legocki A. B.

Acta Biochimica Polonica

|

1981

|

vol. 28

|

issue 2

77-82

10

Identification and synthesis in vitro of plant-specific proteins in yellow lupin root nodules

80%

Stróżycki P., Konieczny A., Legocki A. B.

|

vol. 32

|

issue 1

27-34

11

Translation in vitro of ribonucleic acids from potato virus X, potato virus M and white clover mosaic virus

80%

Szybiak U., Legocki A. B.

Acta Biochimica Polonica

|

1981

|

vol. 28

|

issue 2

99-104

12

Isolation and in vitro translation of leghaemoglobin mRNA from yellow lupin root nodules

80%

Konieczny A., Legocki A. B.

Acta Biochimica Polonica

|

1978

|

vol. 25

|

issue 4

379-390

13

Interaction of elongation factor 2 from wheat germ with guanosine nucleotides and ribosomes

80%

Twardowski T., Legocki A. B.

Acta Biochimica Polonica

|

1977

|

vol. 24

|

issue 1

21-33

14

Yellow lupine gene encoding stearoyl-ACP desaturase - organization, expression and potential application.

71%

Zaborowska Ż., Starzycki M., Femiak I., Świderski M., Legocki A. B.

|

2002

|

vol. 49

|

issue 1

29-42

EN

A gene for the Δturase specific to stearoyl-ACP (acyl carrier protein) was identified from yellow lupine (Lupinus luteus) cDNA and genomic libraries through the differential display method. The desaturase transcript appears in plants infected with Bradyrhizobium sp. (Lupinus) as revealed by Northern hybridization, RT-PCR and expression of β-glucuronidase under the desaturase promoter. A small amount of desaturase transcript was also detected in uninfected plants, which suggests that the gene does not belong to the strict nodule-specific sequences. The desaturase provides unsaturated fatty acids for additional cell membrane synthesis. During nodule and symbiosome development a peribacteroid membrane is formed and the requirement for membrane surface increases, thus the level of desaturase expression is also higher. Transgenic plants of Nicotiana tabacum with overexpression of the full-length lupine stearoyl-ACP desaturase sequence were obtained. They revealed higher content of unsaturated fatty acids (especially oleic acid) in comparison with control plants.

15

Translation and characterization of poly(A)-lacking RNA from lupin root nodules

70%

Konieczny A., Legocki A. B.

Acta Biochimica Polonica

|

1981

|

vol. 28

|

issue 2

83-97

16

Formation of 80S initiation complex in wheat germ system in the presence of poly(AUG)

70%

Golińska B., Legocki A. B.

Acta Biochimica Polonica

|

1979

|

vol. 26

|

issue 4

359-368

17

Lupin leghemoglobins during root nodule development

62%

Szybiak-Stróżycka U., Stróżycki P., Sikorski M., Golińska B., Mądrzak C., Legocki A. B.

|

issue 2

79-85

18

Structure of yellow lupine genes coding for mitotic cyclins

60%

Jeleńska J., Zaborowska Ż., Legocki A. B.

Acta Biochimica Polonica

|

1999

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vol. 46

|

issue 3

759-769

19

Nodule-specific repression of yellow lupin protein R18

60%

Mądrzak C. J., Kulikova O., Legocki A. B.

|

20

An efficient method of genomic DNA isolation from plant tissues

60%

Stróżycki P. M., Legocki A. B.

Acta Biochimica Polonica

|

1995

|

vol. 42

|

issue 3

329-331

Refine search results

Understanding evolution

Reduction of bacterial genome size and expansion resulting from obligate intracellular lifestyle and adaptation to soil habitat.

Glutamine synthetase in Lupinus luteus. Identification and preliminary characterization of nodule-specific cDNA clone

Peptidyltransferase activity of 60S ribosomal subunit from wheat germ

Cloning and identification of lupin nodule specific cDNA sequences

Isolation and classification of a family of cyclin gene homologues in Lupinus luteus

Characterization and expression analysis of the yellow lupin (Lupinus luteus L.) gene coding for nodule specific proline-rich protein.

Reverse transcription and kinetic complexity analysis of mRNA from lupin root nodules

Identification by affinity chromatography of wheat germ ribosomal proteins which interact with RNA

Identification and synthesis in vitro of plant-specific proteins in yellow lupin root nodules

Translation in vitro of ribonucleic acids from potato virus X, potato virus M and white clover mosaic virus

Isolation and in vitro translation of leghaemoglobin mRNA from yellow lupin root nodules

Interaction of elongation factor 2 from wheat germ with guanosine nucleotides and ribosomes

Yellow lupine gene encoding stearoyl-ACP desaturase - organization, expression and potential application.

Translation and characterization of poly(A)-lacking RNA from lupin root nodules

Formation of 80S initiation complex in wheat germ system in the presence of poly(AUG)

Lupin leghemoglobins during root nodule development

Structure of yellow lupine genes coding for mitotic cyclins

Nodule-specific repression of yellow lupin protein R18

An efficient method of genomic DNA isolation from plant tissues