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1

Scaling up the Paulownia tomentosa hairy roots? culture

100%
Pawlowska B., Chmiel A.
Biotechnologia
|
2003
|
issue 4
228-238
EN
Hairy roots, characterized by rapid and stable growth, can be considered as a source of natural plant?s metabolites. The commercial utilization of roots is limited because of the difficulties during the culture in scaling up and designing of bioreactors, which could meet the requirements of optimal conditions of culture. The transformed root cultures of Paulownia tomentosa were conducted in shaking flasks of 300 cm3 containing 80 cm3 hormone-free WP medium, with 3% of sucrose. A mist bioreactor was also designed for transformed roots? cultures. Its glass prototype of 5 dm3 volume was used for optimization of hairy roots of Paulownia tomentosa. In the bioreactor containing 1 dm3 of the medium, after 28 days the dry mass harvest was 10.5-20.5 g depending on culture variants. The total amount of the main metabolite ? verbascoside in these roots was 8601170 mg in one culture (5672 mg/g of dry mass). The research performed on the prototype bioreactor enabled the optimization of its construction. It resulted in a new laboratory bioreactor of 10 dm3 volume made of glass and stainless steel.
2

The effect of the exogenous phenolic compound, caffeic acid on organogenesis of Galanthus elwesii Hook. cultured in vitro

81%
Bach A., Pawlowska B., Hura K.
Biotechnologia
|
2010
|
issue 2
139-145
EN
Caffeic acid is a simple phenolic compound, a phenylpropene derivative with two ?OH groups, which inhibits IAA oxidase and, therefore, is considered to be an auxin cofactor. The aim of the present experiments was to determine the effect of exogenous caffeic acid (100-500 mg dm-3 in medium) on the organogenesis in giant snowdrop (Galanthus elwesii) in in vitro cultures. Exogenous caffeic acid at the supra optimal concentration (500 mg dm-3) inhibited shoots, leaves and roots formation, as well as reduced bulbs fresh mass and caused the explants growing. The lower dose of caffeic acid inhibited the rhizogenesis process. The analysis of the content of phenolic compounds in tissues and organs of giant snowdrop showed that exogenous caffeic acid reduced total content of endogenic phenolic compounds in regenerants.
3

The conditions of transformed roots growth in bioreactors

81%
Pawlowska B., Ledakowicz S., Chmiel A.
Biotechnologia
|
2005
|
issue 2
88-102
EN
Transformed roots are also called transgenic roots or due to their appearance ? hairy roots. Transformed roots are obtained by infection of plants or parts of them with soil bacteria, Agrobacterium rhizogenes. Genetically transformed roots are characterised by rapid and stable growth of biomass, genetic stability, and stable biosynthesis of metabolites. Hairy roots can be an alternative source of many economically important chemicals like enzymes, pigments, flavours and especially bioactive pharmaceuticals. Cultures of transformed roots are comparatively easy to cultivate in shaking flasks. Application of flasks does not allow to obtain great amount of biomass. There is a necessity of scaling up the culture using bioreactors. The first attempts of scaling up the culture were connected with bioreactors designed for microbiological processes. However, these bioreactors are inadequate for hairy roots cultures. Some promising results were achieved in the case of using mist bioreactor. This paper is a review of bioreactors employed in cultures of hairy roots and mathematical modelling of hairy root growth.
4

Subtelomeric rearrangements in Polish subjects with intellectual disability and dysmorphic features

52%
Bogdanowicz J., Pawlowska B., Ilnicka A., Gawlik-Zawislak S., Jozwiak A., Sobiczewska B., Zdzienicka E., Korniszewski L., Zaremba J.
|
|
vol. 51
|
issue 2
215-217
EN
Fluorescent in situ hybridization (FISH) was performed in 76 patients referred to our department because of intellectual disability and dysmorphic features that can be related to subtelomeric microaberrations. In all the patients, conventional cytogenetic methods revealed normal karyotype. Four (5.3%) subtelomeric rearrangements were detected by FISH: 2 subtelomeric 1p36 deletions, an unbalanced translocation involving chromosomes 1 and 12 with 1p36 deletion, and a de novo balanced translocation involving chromosomes 19 and 22. Thus, 3 cases of 1p36 subtelomeric deletion were found (3.95%). To confirm subtelomeric rearrangements in 2 patients, comparative genomic hybridization (CGH) was applied. Moreover, 3 cases of polymorphism without phenotypic effects were found: in 2 patients, the polymorphism involved the long arm of chromosome 2 (maternal derivative in both patients), while in the third patient, a polymorphism of the long arm of chromosome 7 was diagnosed. The latter polymorphism was also found in the patient's mother and grandfather.
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