Search results

Sort By:

Limit search:

Fluorescent in situ hybridization of mitochondrial DNA and RNA

100%

Alán L., Zelenka J., Ježek J., Dlasková A., Ježek P.

Acta Biochimica Polonica

2010

vol. 57

issue 4

403-408

To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules. Future applications are discussed.

Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens

86%

Koníčková R., Jirásková A., Zelenka J., Lešetický L., Štícha M., Vítek L.

Acta Biochimica Polonica

2012

vol. 59

issue 2

289-292

Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.

Refine search results

2 Acta Biochimica Polonica

1 2012

1 2010

2 Zelenka J.

1 Alán L.

1 Dlasková A.

1 Ježek J.

1 Ježek P.

1 Jirásková A.

1 Koníčková R.

1 Lešetický L.

1 Vítek L.

1 Štícha M.

Search results

Fluorescent in situ hybridization of mitochondrial DNA and RNA

Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens