Search results

Sort By:

Limit search:

Influence of extraction methods on the recovery of astragaloside IV from the roots ofAstragalus mongholicusin Soxhlet- and Twisselmann-type apparatus

100%

Kukuła-Koch W., Baj T., Sieniawska E., Koch W., Widelski J., Luchowska K., Kołodziej B., Głowniak K.

Open Chemistry

2015

vol. 13

issue 1

Astragalus mongholicus, commonly used in Traditional Chinese Medicine (TCM), contains significant amounts of triterpene saponins – astragalosides, which possess a wide variety of pharmacological actions, including the treatment of cardiovascular and respiratory diseases. The present study was performed to develop a fast and effective extraction method of saponins from crude plant material using Twisselmann-type apparatus. Moreover, a detailed comparison between Soxhlet and Twisselmann apparatus was performed to explain the advantages and disadvantages of their applications. The content of astragaloside IV – the major saponin of A. mongholicus extracts and chosen as an extraction efficiency marker ‒ was determined by means of RP-HPLCELSD chromatography in an herein optimized method. Twisselmann extraction shortened its duration from 4 to 2 hours in comparison with the Soxhlet method ‒ a generally accepted method preparation for this extract. The effectiveness of both apparatus was alike (3.24 ± 0.44 mg and 3.48 ± 0.26 mg of astragaloside IV/ 4g of powdered root for 2 hours in Twisselmann and 4 hours in Soxhlet apparatus). Compared with a pharmacopoeial method, the application of Twisselmann apparatus enabled a decrease in analysis time of 50%, which is of high significance, especially for large, scaled-up industrial scale.

Refine search results

1 Open Chemistry

1 2015

1 Baj T.

1 Głowniak K.

1 Koch W.

1 Kołodziej B.

1 Kukuła-Koch W.

1 Luchowska K.

1 Sieniawska E.

1 Widelski J.

Search results

Influence of extraction methods on the recovery of astragaloside IV from the roots ofAstragalus mongholicusin Soxhlet- and Twisselmann-type apparatus